CAJ | 학술논문

目的：观察过氧化物酶体增殖物激活受体γ（ PPARγ）激动剂罗格列酮对自体原位肝移植大鼠围术期肠组织中PPARγ表达、NF－κB激活及肠损伤的影响。方法：SD大鼠40只，随机分为对照（ control ）组、假手术（sham）组、原位自体肝移植（OALT）组、罗格列酮（0．3 mg／kg，iv）预处理（ROS＋OALT）组。建立自体原位肝移植模型，在肝脏再灌注后8 h时取肠组织，观察肠组织病理学变化，检测肠组织 PPARγ蛋白表达、NF－κB核转运激活情况、肿瘤坏死因子α（ TNF－α）和白细胞介素6（ IL－6）浓度以及血清二胺氧化酶（ DAO ）和脂肪酸结合蛋白2（FABP2）水平。结果：与sham组相比，OALT组大鼠肠黏膜存在明显的病理学损伤，肠黏膜病理Chiu’s评分明显增高，血清DAO和FABP2升高（P＜0．05）。罗格列酮预处理后，大鼠肠黏膜损伤减轻，肠黏膜病理Chiu’s评分降低，血清DAO和FABP2降低，肠组织PPARγ表达明显上调，NF－κB p65亚基核转位减少，肠组织IL－6和TNF－α浓度降低。结论：自体原位肝移植大鼠围术期炎症反应明显，存在明显的肠道损伤；PPARγ激动剂罗格列酮明显上调PPARγ表达，抑制肠道的炎症反应，减轻自体原位肝移植大鼠围术期的肠损伤。
목적：관찰과양화물매체증식물격활수체γ（ PPARγ）격동제라격렬동대자체원위간이식대서위술기장조직중PPARγ표체、NF－κB격활급장손상적영향。방법：SD대서40지，수궤분위대조（ control ）조、가수술（sham）조、원위자체간이식（OALT）조、라격렬동（0．3 mg／kg，iv）예처리（ROS＋OALT）조。건립자체원위간이식모형，재간장재관주후8 h시취장조직，관찰장조직병이학변화，검측장조직 PPARγ단백표체、NF－κB핵전운격활정황、종류배사인자α（ TNF－α）화백세포개소6（ IL－6）농도이급혈청이알양화매（ DAO ）화지방산결합단백2（FABP2）수평。결과：여sham조상비，OALT조대서장점막존재명현적병이학손상，장점막병리Chiu’s평분명현증고，혈청DAO화FABP2승고（P＜0．05）。라격렬동예처리후，대서장점막손상감경，장점막병리Chiu’s평분강저，혈청DAO화FABP2강저，장조직PPARγ표체명현상조，NF－κB p65아기핵전위감소，장조직IL－6화TNF－α농도강저。결론：자체원위간이식대서위술기염증반응명현，존재명현적장도손상；PPARγ격동제라격렬동명현상조PPARγ표체，억제장도적염증반응，감경자체원위간이식대서위술기적장손상。
AIM: To investigate the effect of rosiglitazone , a peroxisome proliferators-activated receptor γ(PPARγ) agonist, on the expression of PPARγ, the activation of NF-κB and intestine injury in the rats undergoing ortho-topic autologous liver transplantation ( OALT ) .METHODS: Sprague-Dawley male rats were randomly divided into 4 groups:control group, sham group, OALT group and rosiglitazone (0.3 mg/kg, iv) pretreatment (ROS+OALT) group. The OALT model was established , and the intestinal tissues were collected 8 h after the liver reperfusion .The intestinal tis-sue sections were stained to visualize the damage .The expression of PPARγand NF-κB in the tissues, the concentrations of diamine oxidase (DAO) and fatty acid-binding protein 2 (FABP2) in the serum and the concentration of TNF-αand IL-6 in the tissues were measured .RESULTS:Compared with sham group , the intestinal mucosa of the rats showed obvious pathological injury after liver reperfusion in OALT group and ROS group , the Chiu’s scores of intestinal mucosa was signifi-cantly higher , and the serum concentrations of DAO and FABP 2 increased ( P<0.05 ) .After rosiglitazone pretreatment , the injury of intestinal mucosa of the rats was alleviated , the Chiu’s scores was lower and the serum concentrations of DAO and FABP2 decreased (P<0.05), the PPARγexpression was obviously up-regulated in the intestinal tissues, the nuclear translocation of NF-κB was reduced and the concentrations of IL-6 and TNF-αwere decreased .CONCLUSION: During perioperative period of OALT in rats , the inflammatory responses are obvious .Furthermore, obvious intestinal injury oc-curs .PPARγagonist rosiglitazone obviously up-regulates PPARγexpression and inhibits the inflammation in the intestines , thus protecting against intestinal injury in rats undergoing OALT .