CAJ | 학술논문

为了观察GnRH激动剂曲普瑞林对人乳腺癌细胞生长的影响，探讨药物处理后细胞中Fas和FasL基因的表达情况及其与细胞生长的关系，本研究采用了形态学显微镜观察、MTT检测和Real-time PCR技术，对曲普瑞林处理后乳腺癌细胞的生长增殖情况及Fas和FasL基因表达的变化进行了研究。结果显示，曲普瑞林可抑制乳腺癌细胞MCF-7的生长并表现出了剂量依赖性，药物浓度越大对肿瘤细胞的抑制作用越强。随着药物浓度的增大、抑制作用增强，肿瘤细胞中Fas基因的表达量逐渐增大，而FasL基因的表达量却逐渐下降。推测曲普瑞林在作用过程中可能通过促进肿瘤细胞中Fas的表达，诱导了Fas所介导的细胞凋亡途径的恢复，达到抑制肿瘤细胞生长的目的。
위료관찰GnRH격동제곡보서림대인유선암세포생장적영향，탐토약물처리후세포중Fas화FasL기인적표체정황급기여세포생장적관계，본연구채용료형태학현미경관찰、MTT검측화Real-time PCR기술，대곡보서림처리후유선암세포적생장증식정황급Fas화FasL기인표체적변화진행료연구。결과현시，곡보서림가억제유선암세포MCF-7적생장병표현출료제량의뢰성，약물농도월대대종류세포적억제작용월강。수착약물농도적증대、억제작용증강，종류세포중Fas기인적표체량축점증대，이FasL기인적표체량각축점하강。추측곡보서림재작용과정중가능통과촉진종류세포중Fas적표체，유도료Fas소개도적세포조망도경적회복，체도억제종류세포생장적목적。
In order to observe the effect of GnRH aganist triptorelin on the growth of human breast cancer cells, and to explore the expression of Fas and FasL gene in drug treated cells and its relationship with cell growth, the morphological microscope, MTT detection and Real-time PCR were used to study the proliferation and the expression of Fas and FasL gene of breast cancer cells which were treated by the triptorelin. The results showed that triptorelin could inhibit the growth of breast cancer cells MCF-7 and exhibited a characteristic of dose-dependent, the higher the concentration of drugs, the stronger inhibition of tumor cells. It was also observed that the higher the concentration of reagents, the ex-pression of Fas gene in breast cancer cells gradually increased, but the expression of FasL gene was gradually decreased. Therefore, it suggested that the triptorelin might promote the expression of Fas gene in tumor cells, which induced the recovery of the Fas mediated apoptosis pathway, then the growth of tumor cells were inhibited.