中国医药导报
中國醫藥導報
중국의약도보
China Medical Herald
2015年
25期
11-14,19
,共5页
向银洲%彭平%许军%章松勤%宁娜
嚮銀洲%彭平%許軍%章鬆勤%寧娜
향은주%팽평%허군%장송근%저나
人鼻咽癌CNE-2Z细胞%吡咯烷二硫代氨基甲盐酸%核转录因子-资B%增殖细胞核抗原
人鼻嚥癌CNE-2Z細胞%吡咯烷二硫代氨基甲鹽痠%覈轉錄因子-資B%增殖細胞覈抗原
인비인암CNE-2Z세포%필각완이류대안기갑염산%핵전록인자-자B%증식세포핵항원
Human nasopharyngeal carcinoma cell line CNE-2Z%Pyrrolidine dithiocarbamate%NF-κB%Proliferation cell nuclear antigen
目的:研究核转录因子-κB(NF-κB)特异性抑制剂吡咯烷二硫代氨基甲盐酸(PDTC)对人鼻咽癌CNE-2Z细胞生长增殖及其细胞核抗原(PCNA)的影响。方法不同浓度的PDTC(25、50、100μmol/L)作用CNE-2Z细胞不同时间后(24、48、72 h),四甲基偶氮唑蓝(MTT)比色法检测PDTC对CNE-2Z细胞增殖的抑制效应;不同浓度的PDTC(25、50、100μmol/L)作用于CNE-2Z细胞48 h后,流式细胞术(FCM)分析细胞周期,免疫组化(SP)法检测不同强度PDTC作用下CNE-2Z细胞增殖细胞核抗原(PCNA)的表达情况。结果25、50、100μmol/L PDTC处理的CNE-2Z细胞增殖活性明显受到抑制(P<0.05),并呈时间和剂量依赖;流式细胞术结果表明S期细胞数减少,细胞受阻于G0/G1期。与对照(无PDTC处理)比较,PDTC作用后CNE-2Z细胞PCNA表达降低(P<0.05),并呈明显的剂量依赖。结论 PDTC具有显著抑制人鼻咽癌CNE-2Z细胞生长的作用,是一种有潜力的抑制鼻咽癌细胞增殖的药物。
目的:研究覈轉錄因子-κB(NF-κB)特異性抑製劑吡咯烷二硫代氨基甲鹽痠(PDTC)對人鼻嚥癌CNE-2Z細胞生長增殖及其細胞覈抗原(PCNA)的影響。方法不同濃度的PDTC(25、50、100μmol/L)作用CNE-2Z細胞不同時間後(24、48、72 h),四甲基偶氮唑藍(MTT)比色法檢測PDTC對CNE-2Z細胞增殖的抑製效應;不同濃度的PDTC(25、50、100μmol/L)作用于CNE-2Z細胞48 h後,流式細胞術(FCM)分析細胞週期,免疫組化(SP)法檢測不同彊度PDTC作用下CNE-2Z細胞增殖細胞覈抗原(PCNA)的錶達情況。結果25、50、100μmol/L PDTC處理的CNE-2Z細胞增殖活性明顯受到抑製(P<0.05),併呈時間和劑量依賴;流式細胞術結果錶明S期細胞數減少,細胞受阻于G0/G1期。與對照(無PDTC處理)比較,PDTC作用後CNE-2Z細胞PCNA錶達降低(P<0.05),併呈明顯的劑量依賴。結論 PDTC具有顯著抑製人鼻嚥癌CNE-2Z細胞生長的作用,是一種有潛力的抑製鼻嚥癌細胞增殖的藥物。
목적:연구핵전록인자-κB(NF-κB)특이성억제제필각완이류대안기갑염산(PDTC)대인비인암CNE-2Z세포생장증식급기세포핵항원(PCNA)적영향。방법불동농도적PDTC(25、50、100μmol/L)작용CNE-2Z세포불동시간후(24、48、72 h),사갑기우담서람(MTT)비색법검측PDTC대CNE-2Z세포증식적억제효응;불동농도적PDTC(25、50、100μmol/L)작용우CNE-2Z세포48 h후,류식세포술(FCM)분석세포주기,면역조화(SP)법검측불동강도PDTC작용하CNE-2Z세포증식세포핵항원(PCNA)적표체정황。결과25、50、100μmol/L PDTC처리적CNE-2Z세포증식활성명현수도억제(P<0.05),병정시간화제량의뢰;류식세포술결과표명S기세포수감소,세포수조우G0/G1기。여대조(무PDTC처리)비교,PDTC작용후CNE-2Z세포PCNA표체강저(P<0.05),병정명현적제량의뢰。결론 PDTC구유현저억제인비인암CNE-2Z세포생장적작용,시일충유잠력적억제비인암세포증식적약물。
Objective To explore the effects of pyrrolidine dithiocarbamate (PDTC),a specific inhibitor of NF-κB on the proliferation of human nasopharyngeal carcinoma cell line CNE-2Z and its effect on proliferation cell nuclear anti-gen (PCNA) expression. Methods CNE-2Z cells were respectively treated with different concentration (25, 50, 100μmol/L) PDTC and different time (24, 48, 72 h). The inhibition effects of cell proliferation were assayed by four methyl thiazolyl tetrazolium colorimetric(MTT) method. Cell cycle was analyzed by flow cytometry and the expression of prolif-erating cell nuclear antigen (PCNA) was detected by immunocytochemical method after treating CNE-2Z cells with dif-ferent concentration PDTC (25, 50, 100 μmol/L) after 48 h. Results The proliferation activity of CNE-2Z cells dealed with 25, 50, 100μmol/L PDTC was inhibited obviously (P<0.05), and it showed a time-dependent and dose-depen-dent manner. FCM result showed that the cell population reduced in S phase and the cell cycle was arrested in G0/G1 phase. Compared with the control (no PDTC), the expression of PCNA of CNE-2Z cells was down regulated after PDTC dealed with (P<0.05), and it showed a dose-dependent manner. Conclusion The growth of CNE-2Z can be inhibited and the expression of PCNA in CNE-2Z can could be down-regulated by PDTC. PDTC may be a potential chemothera-peutics for the proliferation of nasopharyngeal carcinoma cell line.