CAJ | 학술논문

目的：探讨吸入氢气(H2)对脓毒血症大鼠急性肺损伤的影响。方法选取雄性SD大鼠40只，体重200~250 g。采用随机数字表法将其分为4组(n=10)：假手术组(SH组)、盲肠结扎穿孔组(CLP组)、1% H2组和2%H2组。采用CLP法制备脓毒血症模型。1%H2组与2%H2组于CLP术后12 h分别吸入1%H2与2%H22 h。结束后，采集股动脉血样行血气分析，计算氧合指数(PaO2/FiO2)；测定血浆中高迁移率族蛋白1(HMGB1)、白细胞介素-6(IL-6)水平；随后处死大鼠取肺组织，检测肺组织中Toll样受体4(TLR-4)、核因子-剀B(NF-κB)、髓过氧化物酶(MPO)活性；光镜下观察肺组织病理改变。结果与SH组比较，CLP组、1% H2组、2%H2组PaO2/FiO2降低，血浆中HMGB1、IL-6水平升高，肺组织TLR-4、NF-κB、MPO水平升高，病理学损伤明显加重(P<0.05)。与CLP组比较，1%H2组、2%H2组PaO2/FiO2升高，血浆中HMGB1、IL-6水平降低，肺组织TLR-4、NF-κB、MPO水平降低，病理学损伤也减轻(P<0.05)，1% H2组和2% H2组上述各指标组间比较差异无统计学意义(P >0.05)。结论吸入H2能够减轻脓毒血症引起的急性肺损伤，其机制可能与抑制炎性反应有关。
목적：탐토흡입경기(H2)대농독혈증대서급성폐손상적영향。방법선취웅성SD대서40지，체중200~250 g。채용수궤수자표법장기분위4조(n=10)：가수술조(SH조)、맹장결찰천공조(CLP조)、1% H2조화2%H2조。채용CLP법제비농독혈증모형。1%H2조여2%H2조우CLP술후12 h분별흡입1%H2여2%H22 h。결속후，채집고동맥혈양행혈기분석，계산양합지수(PaO2/FiO2)；측정혈장중고천이솔족단백1(HMGB1)、백세포개소-6(IL-6)수평；수후처사대서취폐조직，검측폐조직중Toll양수체4(TLR-4)、핵인자-개B(NF-κB)、수과양화물매(MPO)활성；광경하관찰폐조직병리개변。결과여SH조비교，CLP조、1% H2조、2%H2조PaO2/FiO2강저，혈장중HMGB1、IL-6수평승고，폐조직TLR-4、NF-κB、MPO수평승고，병이학손상명현가중(P<0.05)。여CLP조비교，1%H2조、2%H2조PaO2/FiO2승고，혈장중HMGB1、IL-6수평강저，폐조직TLR-4、NF-κB、MPO수평강저，병이학손상야감경(P<0.05)，1% H2조화2% H2조상술각지표조간비교차이무통계학의의(P >0.05)。결론흡입H2능구감경농독혈증인기적급성폐손상，기궤제가능여억제염성반응유관。
Objective To investigate the effect of hydrogen (H2) inhalation on acute lung injury in rats with sepsis. Methods Forty healthy male SD rats (weighing 200-250 g) were randomly divided into 4 groups (n = 10) using a ran-dom number table:sham operation group (SH group), cecal ligation and puncture group (CLP group), 1% H2 group, 2%H2 group respectively. Sepsis model was produced by CLP. 1% H2 and 2% H2 group received 2 h inhalation of 1% H2 and 2% H2 at 12 h after CLP, respectively. At the end of hydrogen inhalation, blood samples were obtained for blood gas analysis. PaO2/FiO2 was calculated and the levels of high mobility group protein 1 (HMGB1), interleukin-6 (IL-6) were determined. The rats were then sacrificed and lungs were immediately removed for determination of the activity of Toll-like receptors-4 (TLR-4), nuclear factor kappa B (NF-κB) and myeloperoxidase (MPO); pathology assessment of lung was done under light microscope. Results Compared with SH group, PaO2/FiO2 was significantly decreased, plasma levels of HMGB1, IL-6 increased, levels of TLR-4, NF-κB, MPO in lungs increased, and the pathological injury was significantly aggravated in CLP group, 1%H2 group and 2% H2 group (P<0.05). Compared with CLP group, PaO2/FiO2 increased significantly, levels of HMGB1, IL-6 in plasma decreased markedly, levels of TLR-4, NF-κB, MPO in lungs decreased, and the pathological injury was reduced in 1%H2 group and 2%H2 group (P<0.05). There were no signifi-cant differences of the above indexes between 1% H2 group and 2% H2 group (P>0.05). Conclusion Hydrogen inhala-tion can alleviate sepsis induced ALI in rats, and its mechanism may be related to inhibiting inflammatory response.