微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
3期
48-53
,共6页
沈智超%刘芡芡%晁艳%罗兵%王云
瀋智超%劉芡芡%晁豔%囉兵%王雲
침지초%류검검%조염%라병%왕운
EB病毒%基因多态性%病毒白介素-10基因%鼻咽癌
EB病毒%基因多態性%病毒白介素-10基因%鼻嚥癌
EB병독%기인다태성%병독백개소-10기인%비인암
Epstein-Barr virus(EBV)%gene polymorphism%viral interleukin-10(vIL-10) gene%nasopharyngeal carcinoma(NPC)
EB病毒（ Epstein-Barr virus，EBV）基因变异与EBV相关疾病发生的关系尚无明确结论，本研究旨在了解广东地区人群中EBV白介素-10基因（viral interleukin-10，vIL-10）基因变异特征，探讨其变异与鼻咽癌（ nasopharyngeal carcinoma，NPC）发生的关系。采用PCR和DNA测序检测NPC组织和健康成年人咽漱液中vIL-10序列。50例NPC和70例健康人群完成序列测定，其中42例（84．0％）NPC和60例（85．7％）健康人群与EBV标准株B95-8氨基酸序列一致，被分类为B95-8型；剩余8例（16．0％）NPC和10例（14．3％）健康人群在信号肽区域出现氨基酸突变，被分类为SPM变异型。2种型别及4处共有突变在2种人群中的分布无统计学意义（P＞0．05）。结果表明，广东地区NPC和健康人群中EBV vIL-10高度保守，均以B95-8型为主，而SPM变异型的地域性分布及与鼻咽癌的确切关系有待进一步研究。
EB病毒（ Epstein-Barr virus，EBV）基因變異與EBV相關疾病髮生的關繫尚無明確結論，本研究旨在瞭解廣東地區人群中EBV白介素-10基因（viral interleukin-10，vIL-10）基因變異特徵，探討其變異與鼻嚥癌（ nasopharyngeal carcinoma，NPC）髮生的關繫。採用PCR和DNA測序檢測NPC組織和健康成年人嚥漱液中vIL-10序列。50例NPC和70例健康人群完成序列測定，其中42例（84．0％）NPC和60例（85．7％）健康人群與EBV標準株B95-8氨基痠序列一緻，被分類為B95-8型；剩餘8例（16．0％）NPC和10例（14．3％）健康人群在信號肽區域齣現氨基痠突變，被分類為SPM變異型。2種型彆及4處共有突變在2種人群中的分佈無統計學意義（P＞0．05）。結果錶明，廣東地區NPC和健康人群中EBV vIL-10高度保守，均以B95-8型為主，而SPM變異型的地域性分佈及與鼻嚥癌的確切關繫有待進一步研究。
EB병독（ Epstein-Barr virus，EBV）기인변이여EBV상관질병발생적관계상무명학결론，본연구지재료해엄동지구인군중EBV백개소-10기인（viral interleukin-10，vIL-10）기인변이특정，탐토기변이여비인암（ nasopharyngeal carcinoma，NPC）발생적관계。채용PCR화DNA측서검측NPC조직화건강성년인인수액중vIL-10서렬。50례NPC화70례건강인군완성서렬측정，기중42례（84．0％）NPC화60례（85．7％）건강인군여EBV표준주B95-8안기산서렬일치，피분류위B95-8형；잉여8례（16．0％）NPC화10례（14．3％）건강인군재신호태구역출현안기산돌변，피분류위SPM변이형。2충형별급4처공유돌변재2충인군중적분포무통계학의의（P＞0．05）。결과표명，엄동지구NPC화건강인군중EBV vIL-10고도보수，균이B95-8형위주，이SPM변이형적지역성분포급여비인암적학절관계유대진일보연구。
There is no tangible conclusion between the gene mutation of Epstein-Barr virus ( EBV) associate with EBV-associated diseases.The purpose of the study is to understand the specific mutation features of the viral interleu-kin-10 (vIL-10) gene among the press in Guangdong and investigate the relation between the occurrence of nasopha-ryngeal carcinoma ( NPC) and the vIL-10 gene mutation features.PCR and DNA sequencing were adopted to test the vIL-10 of sequences of NPC tissue and healthy adults'pharynx gargle.Sequences determination was completed with samples of 50 NPCs and 70 healthy donors.Among them, sequences of 42 cases (84.0%) NPC cases and sequences of 60 (85.7%) cases of healthy press were in consistent with identical amino acid sequences to B95-8 prototype, and classified into B95-8 type, while the remaining 8 (16.0%) NPC isolates and 10 cases (14.3%) of healthy press presented amino acid mutations at the signal peptide region were classified into SPM mutation type.The distributions of the two types as well as the four common mutations of vIL-10 gene between NPC and healthy press had no significant difference statistically (P>0.05).The results indicated that the vIL-10 gene is highly conserved and B95-8 is the main type among EBV isolates in NPC and healthy press from Guangdong Province.However, the regional distribution of SPM mutation and the exact relationship between SPM mutation and NPC remain a pending further research.
