CAJ | 학술논문

本文旨在筛选出适合天然牧草青贮的甲酸、纤维素酶、乳酸菌剂添加浓度,为天然牧草添加剂青贮提供科学依据. 试验采用单因子完全随机设计,针对天然牧草青贮添加甲酸(3.00、6.00、9.00 mL/kg)、纤维素酶(0.05、0.10、0.15 g/kg)、乳酸菌剂(0.01、0.02、0.03 g/kg)3种不同添加剂,设置无任何添加为对照组,对不同剂量下天然牧草青贮饲料的感官品质、发酵品质、营养成分及体外消化情况进行比较. 结果表明:1)甲酸添加6.0 mL/kg组感官品质得到明显改善,pH、氨态氮/总氮显著低于对照组( P<0.05) ,干物质、粗蛋白质、可溶性碳水化合物含量显著高于对照组( P<0.05) ,中性洗涤纤维( NDF)和酸性洗涤纤维( ADF)含量与对照组差异不显著(P>0.05),总能、消化能显著高于对照组(P<0.05),体外干物质消化率与对照组差异不显著( P>0.05). 2)纤维素酶添加0.15 g/kg组的感官品质明显好于对照组,pH、氨态氮/总氮显著低于对照组(P<0.05),干物质、粗蛋白质、粗脂肪、可溶性碳水化合物含量显著高于对照组(P<0.05) ,NDF和ADF含量显著低于对照组( P<0.05) ,总能、消化能、体外干物质消化率显著高于对照组( P<0.05). 3)乳酸菌剂添加0.02 g/kg组感官品质明得到显改善,pH、氨态氮/总氮显著低于对照组( P<0.05) ,乳酸含量显著高于对照组( P<0.05) ,干物质、粗蛋白质、粗脂肪含量显著高于对照组(P<0.05),NDF和ADF含量显著低于对照组(P>0.05),总能、消化能、体外干物质消化率显著高于对照组( P<0.05). 综合考虑,甲酸添加6.0 mL/kg,纤维素酶添加0.15 g/kg,乳酸菌剂添加0.02 g/kg,所得的天然牧草青贮饲料在保证良好的发酵品质的同时还可以有效保存天然牧草中的营养价值.
본문지재사선출괄합천연목초청저적갑산、섬유소매、유산균제첨가농도,위천연목초첨가제청저제공과학의거. 시험채용단인자완전수궤설계,침대천연목초청저첨가갑산(3.00、6.00、9.00 mL/kg)、섬유소매(0.05、0.10、0.15 g/kg)、유산균제(0.01、0.02、0.03 g/kg)3충불동첨가제,설치무임하첨가위대조조,대불동제량하천연목초청저사료적감관품질、발효품질、영양성분급체외소화정황진행비교. 결과표명:1)갑산첨가6.0 mL/kg조감관품질득도명현개선,pH、안태담/총담현저저우대조조( P<0.05) ,간물질、조단백질、가용성탄수화합물함량현저고우대조조( P<0.05) ,중성세조섬유( NDF)화산성세조섬유( ADF)함량여대조조차이불현저(P>0.05),총능、소화능현저고우대조조(P<0.05),체외간물질소화솔여대조조차이불현저( P>0.05). 2)섬유소매첨가0.15 g/kg조적감관품질명현호우대조조,pH、안태담/총담현저저우대조조(P<0.05),간물질、조단백질、조지방、가용성탄수화합물함량현저고우대조조(P<0.05) ,NDF화ADF함량현저저우대조조( P<0.05) ,총능、소화능、체외간물질소화솔현저고우대조조( P<0.05). 3)유산균제첨가0.02 g/kg조감관품질명득도현개선,pH、안태담/총담현저저우대조조( P<0.05) ,유산함량현저고우대조조( P<0.05) ,간물질、조단백질、조지방함량현저고우대조조(P<0.05),NDF화ADF함량현저저우대조조(P>0.05),총능、소화능、체외간물질소화솔현저고우대조조( P<0.05). 종합고필,갑산첨가6.0 mL/kg,섬유소매첨가0.15 g/kg,유산균제첨가0.02 g/kg,소득적천연목초청저사료재보증량호적발효품질적동시환가이유효보존천연목초중적영양개치.
This experiment was conducted to selected the suitable supplemental concentrations of formic acid ( FA) , cellulose ( CE) and lactic acid bacteria ( LAB) for natural grass silage, and to provide a scientific ba-sis for forage grass silage additives. A single factor completely randomized was uesd in the experimen, added FA (3.00, 6.00 and 9.00 mL/kg), CE (0.05, 0.10 and 0.15 g/kg) and LAB (0.01, 0.02 and 0.03 g/kg) of 3 different kinds of additives in natural grass forage, and the control group ( CK) was without any addition. Analyzed the sensory quality, fermentation quality, nutrient composition and in vitro digestibility of natural forge silage nder different additives. The results showed as follows:1) added 6.0 mL/kg FA group had been significantly improved the sensory quality, the pH, ammoniacal nitrogen/total nitrogen ( AN/TN) were signif-icantly lower than CK (P<0.05), the contents of dry matter (DM), crude protein (CP) and water soluble carbohydrates ( WSC) was significantly higher than those of CK ( P<0.05) , the contents of neutral detergent fibre ( NDF) and acid detergent fibre ( ADF) had no significant difference compared with CK ( P>0.05) , the in vitro digestibility of gross energy ( GE) and digestive energy ( DE) was significantly higher than CK ( P<0.05) , the in vitro dry matter digestibility ( IVDMD) had no significant difference compared with CK ( P>0.05). 2) The sensory quality of added 0.15 g/kg CE group had been significantly better than the control group, the pH, AN/TN were significantly lower than CK ( P<0.05) , the contents of DM, CP, ether extract ( EE) and WSC were significantly higher than CK ( P<0.05) , the contents of NDF and ADF were significant-ly lower than CK ( P<0.05) , the GE, DE and IVDMD were significantly higher than CK ( P<0.05) . 3) The sensory quality of added 0.02 g/kg LAB group had been significantly improved, the pH, AN/TN were signifi-cantly lower than CK ( P<0.05) , the lactic acid content was significantly higher than CK ( P<0.05) , the con-tents of DM, CP, EE were significantly higher than CK ( P<0.05) , the content of NDF and ADF was signifi-cantly lower than CK ( P<0.05) , the GE, DE and IVDMD were significantly higher than CK ( P<0.05) . Ac-cording to principal component analysis, added 6.0 mL/kg FA, 0.1g/kg CE or 0.02 g/kg LAB, the natural grass silage fermentation can guarantee good quality, and can effectively save the nutritional value of forage.