安徽工程大学学报
安徽工程大學學報
안휘공정대학학보
Journal of Anhui Polytechnic University
2015年
4期
17-19
,共3页
粘杆菌素%流加补料%发酵
粘桿菌素%流加補料%髮酵
점간균소%류가보료%발효
colistin%flow feeding%fermentation
粘杆菌素发酵老工艺发酵周期短,发酵单位比较低。研究了流加补糖新工艺,通过发酵过程流加葡萄糖,解除了物料浓度过高抑制粘杆菌素合成速率的问题。同时结合对发酵接种量、培养温度等参数的优化,使粘杆菌素发酵单位由110000 U/mL 提高至520000 U/mL,并把该工艺成功地运用到生产中。
粘桿菌素髮酵老工藝髮酵週期短,髮酵單位比較低。研究瞭流加補糖新工藝,通過髮酵過程流加葡萄糖,解除瞭物料濃度過高抑製粘桿菌素閤成速率的問題。同時結閤對髮酵接種量、培養溫度等參數的優化,使粘桿菌素髮酵單位由110000 U/mL 提高至520000 U/mL,併把該工藝成功地運用到生產中。
점간균소발효로공예발효주기단,발효단위비교저。연구료류가보당신공예,통과발효과정류가포도당,해제료물료농도과고억제점간균소합성속솔적문제。동시결합대발효접충량、배양온도등삼수적우화,사점간균소발효단위유110000 U/mL 제고지520000 U/mL,병파해공예성공지운용도생산중。
The old fermentation process of colistin has the disadvantages of short fermentation period and low fermentation unit.This paper developed a new sugar supplement test for colistin and studied the fed sugar fermentation process of colistin.By using fed glucose fermentation process,the inhibition of colistin synthesis rate due to the high glucose concentration can be relieved.Combined with the optimization of fermentation inoculum and culture temperature parameter,the colistin fermentation unit can go up from 1 10 000 U/mL to 520 000 U/mL.And this technology has been successfully applied to large-scale pro-duction.
