CAJ | 학술논문

目的：研究补肾活血方对糖尿病（DM）性勃起功能障碍（ED）大鼠勃起功能的疗效及其作用机理。方法将40只雄性 SD 大鼠随机分为正常组、糖尿病模型组、补肾活血方低剂量组、补肾活血方高剂量组，每组10只。糖尿病模型组、补肾活血方低剂量组、补肾活血方高剂量组3组大鼠腹腔注射链脲佐菌素（STZ），构建糖尿病大鼠模型。正常组和糖尿病模型组进行生理盐水灌胃，补肾活血方低剂量组（3 g／kg）和高剂量组（6 g／kg）进行补肾活血方灌胃，疗程8周，8周后取材测定阴茎脏器指数和一抗、二抗浓度，采用 Biopack 电生理仪检测阴茎海绵体内压（ICP），蛋白印迹法检测蛋白激酶 B（AKT）、磷酸化蛋白激酶 B（pAKT）表达，TUNEL 法检测阴茎组织凋亡，Masson 三色染色观察阴茎组织病理改变。结果与糖尿病模型组比较，补肾活血方低、高剂量组阴茎脏器指数无明显差异（P ＞0．05），补肾活血方高剂量组 ICP 和 ICP 与平均动脉压（MAP）的比值明显升高（P ＜0．05）。与糖尿病模型组比较，补肾活血方低、高剂量组 pAKT、AKT 表达显著增加（P ＜0．05），并且阴茎海绵体平滑肌凋亡率均有所下降，但差异无显著性意义（P ＞0．05）。补肾活血方低剂量组与糖尿病模型组相比，胶原纤维比例显著下降（P ＜0．05）。结论补肾活血方可增加糖尿病大鼠阴茎 ICP，其机理与提高 pAKT 表达、抑制阴茎海绵体平滑肌细胞凋亡、降低胶原纤维的生成有关。
목적：연구보신활혈방대당뇨병（DM）성발기공능장애（ED）대서발기공능적료효급기작용궤리。방법장40지웅성 SD 대서수궤분위정상조、당뇨병모형조、보신활혈방저제량조、보신활혈방고제량조，매조10지。당뇨병모형조、보신활혈방저제량조、보신활혈방고제량조3조대서복강주사련뇨좌균소（STZ），구건당뇨병대서모형。정상조화당뇨병모형조진행생리염수관위，보신활혈방저제량조（3 g／kg）화고제량조（6 g／kg）진행보신활혈방관위，료정8주，8주후취재측정음경장기지수화일항、이항농도，채용 Biopack 전생리의검측음경해면체내압（ICP），단백인적법검측단백격매 B（AKT）、린산화단백격매 B（pAKT）표체，TUNEL 법검측음경조직조망，Masson 삼색염색관찰음경조직병리개변。결과여당뇨병모형조비교，보신활혈방저、고제량조음경장기지수무명현차이（P ＞0．05），보신활혈방고제량조 ICP 화 ICP 여평균동맥압（MAP）적비치명현승고（P ＜0．05）。여당뇨병모형조비교，보신활혈방저、고제량조 pAKT、AKT 표체현저증가（P ＜0．05），병차음경해면체평활기조망솔균유소하강，단차이무현저성의의（P ＞0．05）。보신활혈방저제량조여당뇨병모형조상비，효원섬유비례현저하강（P ＜0．05）。결론보신활혈방가증가당뇨병대서음경 ICP，기궤리여제고 pAKT 표체、억제음경해면체평활기세포조망、강저효원섬유적생성유관。
Objective To study the therapeutic effect and mechanism of invigorating kidney and activa-ting blood(IKAB)recipe,a Chinese herb compound,on diabetic erectile dysfunction (DED)in rats. Methods 40 male SD rats were randomly divided into four groups (each n =10):normal group,model group,low-dose IKAB group (low-dose group)and high-dose IKAB group(high-dose group).The rat model with 2 type of diabet mellitus was induced by intraperitoneal injection of streptozotocin (STZ). Normal group and model group were intragastrically administered (ig.)with normal saline,low-dose group was ig.3 g/kg of IKAB and high-dose group was ig.6 g/kg IKAB for consecutive 8 weeks.The penis viscera index was measured;the intracavernous pressure (ICP)were detected by Biopack electro-physiological instrument;the expressions of pAKT and AKT were detectd by Western blotting;cell apop-tosis of corpus cavernosum smooth muscle were measured by using TUNEL method and the pathological changes in penile tissue were observed by using Masson trichrome staining.Results Compared with model group,the penis viscera index of both IKAB groups had no statistical differences (P >0.05);the expressions of pAKT and AKT increased significantly(P <0.05);although the ratio of cell apoptosis of corpus cavernosum smooth muscle decreased,the statistical differences were not shown (P >0.05).Fur-thermore,ICP and ICP/MAP increased markedly in high-dose group and the collagen fiber proportion de-creased in low -dose group compared with model group (P <0.05).Conclusion The mechanism of in-vigorating kidney and activating blood recipe increasing ICP in rat model with DMis related to increasing the expression of pAKT,inhibiting cell apoptosis of corpus cavernosum smooth muscle and reducing colla-gen fibers formation in penis.