CAJ | 학술논문

目的：研究血管内皮细胞中锌指核转录因子（ ZNF580）对内皮型一氧化氮合酶（ eNOS）表达的影响。方法通过基因芯片技术检测ZNF580过表达的人血管内皮杂交瘤细胞系（EA．hy926）中基因表达谱的变化。采用实时定量聚合酶链反应（RT-PCR）和免疫印迹试验（Western bolt）检测转染了携带有ZNF580的慢病毒载体（Lenti-GFP-ZNF580）和携带ZNF580小干扰RNA慢病毒载体（Lenti-RNAi-ZNF580）的EA．hy926中ZNF580和eNOS mRNA和蛋白的表达变化。结果多种基因表达在ZNF580过表达的EA．hy926细胞中发生了改变，其中eNOS等基因的表达明显上调。转染了携带有（Lenti-RNAi-ZNF580）的内皮细胞中ZNF580和eNOS表达显著下调，而转染了Lenti -GFP-ZNF580的内皮细胞中ZNF580和eNOS表达显著上调。结论转录因子ZNF580促进了血管内皮细胞中eNOS的表达。
목적：연구혈관내피세포중자지핵전록인자（ ZNF580）대내피형일양화담합매（ eNOS）표체적영향。방법통과기인심편기술검측ZNF580과표체적인혈관내피잡교류세포계（EA．hy926）중기인표체보적변화。채용실시정량취합매련반응（RT-PCR）화면역인적시험（Western bolt）검측전염료휴대유ZNF580적만병독재체（Lenti-GFP-ZNF580）화휴대ZNF580소간우RNA만병독재체（Lenti-RNAi-ZNF580）적EA．hy926중ZNF580화eNOS mRNA화단백적표체변화。결과다충기인표체재ZNF580과표체적EA．hy926세포중발생료개변，기중eNOS등기인적표체명현상조。전염료휴대유（Lenti-RNAi-ZNF580）적내피세포중ZNF580화eNOS표체현저하조，이전염료Lenti -GFP-ZNF580적내피세포중ZNF580화eNOS표체현저상조。결론전록인자ZNF580촉진료혈관내피세포중eNOS적표체。
Objective The effect of the novel zinc-finger nuclear transcription factor ( ZNF580 ) on the expression of endothelial nitric oxide synthase ( eNOS) in vascular endothelial cells is discussed.Methods The changes of gene expression profiling of ZNF580-overexpressed EA.hy926 cells were detected by gene microarray.The ZNF580 and eNOS expressions of the EA.hy926 endothelial cells which transfected with Lenti-GFP-ZNF580 and Lenti-RNAi-ZNF580 were detected by the real-time polymerase chain reaction (RT-PCR) and Western bolt.Results The results of gene chip showed that there were many gene expression changes in ZNF580-overexpressed EA.hy926 cells. The expression of eNOS was markedly up-regulated.The mRNA and protein expressions of ZNF580 and eNOS were increased in EA.hy926 cells transfected with Lenti-GFP-ZNF580.But the eNOS and ZNF580 expressions were down-regulated in EA.hy926 cells transfected with Lenti-RNAi-ZNF580.Conclusion Transcriptional factor ZNF580 promotes the expression of eNOS in vascular endothelial cells.