中华神经外科疾病研究杂志
中華神經外科疾病研究雜誌
중화신경외과질병연구잡지
Chinese Journal of Neurosurgical Disease Research
2015年
4期
293-296
,共4页
何骏驰%刘旻谛%刘振%罗良生
何駿馳%劉旻諦%劉振%囉良生
하준치%류민체%류진%라량생
重组骨桥蛋白%蛛网膜下腔出血%脑血管痉挛%内皮素-1%一氧化氮合酶
重組骨橋蛋白%蛛網膜下腔齣血%腦血管痙攣%內皮素-1%一氧化氮閤酶
중조골교단백%주망막하강출혈%뇌혈관경련%내피소-1%일양화담합매
Recombinant osteopontin%Subarachnoid hemorrhage%Cerebral vasospasm%Endothelin-1%Nitric oxide synthase
目的：探究重组骨桥蛋白（ r-OPN）对蛛网膜下腔出血（ SAH）后脑血管痉挛（ CVS）的作用及其可能机制。方法将48只大鼠随机分为假手术＋安慰剂组、SAH＋安慰剂组、SAH＋低剂量r-OPN组和SAH＋高剂量r-OPN组。采用枕大池二次注血法建立SAH模型。首次注血后72 h取脑脊液，处死大鼠，通过测量基底动脉横截面积和管壁厚度判断脑血管痉挛情况，采用酶联免疫吸附法（ELISA）检测脑脊液中内皮素-1（ET-1）水平， Western blot测定基底动脉磷酸化内皮型一氧化氮合酶（ p-eNOS）和诱导型一氧化氮合酶（ iNOS）水平。结果与SAH＋安慰剂组相比，SAH＋高剂量r-OPN组大鼠基底动脉横截面积明显增加，管壁厚度明显减轻，脑脊液ET-1水平明显降低，基底动脉p-eNOS表达明显升高，iNOS表达明显降低。结论 r-OPN能有效缓解SAH后CVS，其机制可能与抑制ET-1的产生、降低iNOS的表达，提高p-eNOS的表达有关。
目的：探究重組骨橋蛋白（ r-OPN）對蛛網膜下腔齣血（ SAH）後腦血管痙攣（ CVS）的作用及其可能機製。方法將48隻大鼠隨機分為假手術＋安慰劑組、SAH＋安慰劑組、SAH＋低劑量r-OPN組和SAH＋高劑量r-OPN組。採用枕大池二次註血法建立SAH模型。首次註血後72 h取腦脊液，處死大鼠，通過測量基底動脈橫截麵積和管壁厚度判斷腦血管痙攣情況，採用酶聯免疫吸附法（ELISA）檢測腦脊液中內皮素-1（ET-1）水平， Western blot測定基底動脈燐痠化內皮型一氧化氮閤酶（ p-eNOS）和誘導型一氧化氮閤酶（ iNOS）水平。結果與SAH＋安慰劑組相比，SAH＋高劑量r-OPN組大鼠基底動脈橫截麵積明顯增加，管壁厚度明顯減輕，腦脊液ET-1水平明顯降低，基底動脈p-eNOS錶達明顯升高，iNOS錶達明顯降低。結論 r-OPN能有效緩解SAH後CVS，其機製可能與抑製ET-1的產生、降低iNOS的錶達，提高p-eNOS的錶達有關。
목적：탐구중조골교단백（ r-OPN）대주망막하강출혈（ SAH）후뇌혈관경련（ CVS）적작용급기가능궤제。방법장48지대서수궤분위가수술＋안위제조、SAH＋안위제조、SAH＋저제량r-OPN조화SAH＋고제량r-OPN조。채용침대지이차주혈법건립SAH모형。수차주혈후72 h취뇌척액，처사대서，통과측량기저동맥횡절면적화관벽후도판단뇌혈관경련정황，채용매련면역흡부법（ELISA）검측뇌척액중내피소-1（ET-1）수평， Western blot측정기저동맥린산화내피형일양화담합매（ p-eNOS）화유도형일양화담합매（ iNOS）수평。결과여SAH＋안위제조상비，SAH＋고제량r-OPN조대서기저동맥횡절면적명현증가，관벽후도명현감경，뇌척액ET-1수평명현강저，기저동맥p-eNOS표체명현승고，iNOS표체명현강저。결론 r-OPN능유효완해SAH후CVS，기궤제가능여억제ET-1적산생、강저iNOS적표체，제고p-eNOS적표체유관。
Objective The effect of recombinant osteopontin (r-OPN) against cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) is investigated and the potential mechanisms are discussed.Methods Forty-eight rats were randomly assigned to sham+vehicle group (n=12), SAH+vehicle group (n=12), SAH+OPN 0.03 (0.03μg) group (n=12), and SAH+OPN 0.1 (0.1μg) group (n=12).The double injection model of cisterna magna was induced.Cerebrospinal fluid ( CSF ) was collected and rats were sacrificed at 72 h after the first hemorrhage induction.The degree of CVS was determined by the measurement of the cross-sectional area and thickness of basilar arteries (BA) under Hematoxylin-eosin (HE) staining.The level of endothelin-1 (ET-1) in CSF was tested using an enzyme-linked immunosorbent assays (ELISA) kit.Western blot analysis was applied to evaluate the expressions of phosphorylated endothelial nitric oxide synthase ( p-eNOS ) and inducible nitric oxide synthase (iNOS) in BA.Results The SAH+OPN 0.1 group showed significant increased cross-sectional area, decreased wall thickness, declined ET-1 level in CSF, increased expression of p-eNOS, and decreased expression of iNOS in BA, compared with the SAH+vehicle group.Conclusion r-OPN is demonstrated to attenuate CVS, and its possible mechanism may be involved in the suppression of ET-1 production and iNOS expression, and induction of p-eNOS expression.
