CAJ | 학술논문

目的建立同时测定痰热清注射液中新绿原酸、绿原酸、咖啡酸、野黄芩苷、芦丁、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷的高效液相色谱法.方法采用Agilent TC-C18(2)色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%甲酸溶液(B)梯度洗脱,流速为1.0 ml/min,柱温30℃,进样量20μl,检测波长280 nm.结果 9种有效成分的线性关系良好,精密度、重复性、稳定性的RSD值均低于2%,加样回收率为99.3%~103.1%.结论本方法简便、准确、重复性好,可有效控制痰热清注射液的质量.
목적 건립동시측정담열청주사액중신록원산、록원산、가배산、야황금감、호정、이록원산A、이록원산C、황금감、한황금감적고효액상색보법.방법채용Agilent TC-C18(2)색보주(250 mm×4.6 mm,5μm),이을정(A)-0.1%갑산용액(B)제도세탈,류속위1.0 ml/min,주온30℃,진양량20μl,검측파장280 nm.결과 9충유효성분적선성관계량호,정밀도、중복성、은정성적RSD치균저우2%,가양회수솔위99.3%~103.1%.결론 본방법간편、준학、중복성호,가유효공제담열청주사액적질량.
Objective To establish HPLC method for the simultaneous detgrmination of 9 effective components ofTanreqing injection.Methods The Agilent TC-C18 (2) (250 mm×4.6 mm, 5μm) column was used, the mobile phase consisted of acetonitrile (A) acetonitrile-0.1% formic acid aqueous solution (B) in a gradient mode, the flow rate was 1.0 ml/min,the column temperature was 30℃, the injection volume was 10μl, and the detecting wavelength was at 280 nm.Results All the 9 effective components showed a good linear relationship. TheRSDof the precision, reproducibility and stability tests were less than 2%. The average recoveries of the 9 effective components were in the range of 99.3%-103.1%.Conclusion This analysis method is simple, accurate and has a good specificity, which can be suitable for controlling the quality of Tanreqing injection.