CAJ | 학술논문

采用平板水解圈法（ plate hydrolysis spot method）对分离自酒鬼酒发酵车间空气样品的细菌进行淀粉酶产生菌筛选，运用基于16S rRNA基因序列的分析方法对高酶活菌株进行系统发育多样性分析。结果表明，73个受试菌株中，有23株为淀粉酶产生菌，占受试菌株的31．5％，其中有9株为高酶活菌。23个淀粉酶产生菌类群多样性和物种多样性较高，属于4个大的系统发育类群（ Actinobacteria、Deinococcus-Thermus、Firmi-cutes、Proteobacteria ）中的10个科（ Bacillaceae、Deinococcaceae、Intrasporangiaceae、Microbacteriaceae、Micrococ-caceae、Nocardiaceae、Propionibacteriaceae、Pseudomonadaceae、Rhodobacteraceae、Xanthomonadaceae）的13个属，可分为21个物种。进一步分析表明，9株高酶活菌属于细菌域（ Eubacteria）的4个大的系统发育类群（ Acti-nobacteria、Deinococcus-Thermus、Firmicutes、Proteobacteria）的7个科（ Bacillaceae、Deinococcaceae、Micrococcace-ae、Microbacteriaceae、Nocardiaceae、Rhodobacteraceae、Xanthomonadaceae），归属于8个属。研究结果表明，酒鬼酒发酵车间空气源细菌存在较高比例的淀粉酶产生菌，且这些菌株具有较高的类群多样性和物种多样性。
채용평판수해권법（ plate hydrolysis spot method）대분리자주귀주발효차간공기양품적세균진행정분매산생균사선，운용기우16S rRNA기인서렬적분석방법대고매활균주진행계통발육다양성분석。결과표명，73개수시균주중，유23주위정분매산생균，점수시균주적31．5％，기중유9주위고매활균。23개정분매산생균류군다양성화물충다양성교고，속우4개대적계통발육류군（ Actinobacteria、Deinococcus-Thermus、Firmi-cutes、Proteobacteria ）중적10개과（ Bacillaceae、Deinococcaceae、Intrasporangiaceae、Microbacteriaceae、Micrococ-caceae、Nocardiaceae、Propionibacteriaceae、Pseudomonadaceae、Rhodobacteraceae、Xanthomonadaceae）적13개속，가분위21개물충。진일보분석표명，9주고매활균속우세균역（ Eubacteria）적4개대적계통발육류군（ Acti-nobacteria、Deinococcus-Thermus、Firmicutes、Proteobacteria）적7개과（ Bacillaceae、Deinococcaceae、Micrococcace-ae、Microbacteriaceae、Nocardiaceae、Rhodobacteraceae、Xanthomonadaceae），귀속우8개속。연구결과표명，주귀주발효차간공기원세균존재교고비례적정분매산생균，차저사균주구유교고적류군다양성화물충다양성。
Bacteria collected from air in a liquor fermentation workshop in Jiugui Liquor Co.Ltd., China, were iso-lated using plate hydrolysis spot method and screened for their amylase activity.The high amylase active strains were analyzed with 16S rRNA for their diversities of phylogeny.The results showed that 23 out of 73 (31.5%) strains test-ed were positive in amylase production.Among them 9 strains possessed fairly strong amylase activities.The results based on 16S rRNA phylogenetic analyses showed that the 23 amylase positive strains highly had group and species di-versities belonging to 13 genera ( Arsenicicoccus, Arthrobacter, Bacillus, Deinococcus, Janibacter, Kocuria, Luteococ-cus, Microbacterium, Micrococcus, Paracoccus, Pseudomonas, Rhodococcus, Xanthomonas) of 10 families ( Bacillace-ae, Deinococcaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Nocardiaceae, Propionibacteriaceae, Pseudomonadaceae, Rhodobacteraceae, Xanthomonadaceae) in 4 phylogenetic groups ( Actinobacteria, Deinococcus-Thermus, Firmicutes, Proteobacteria) and subdivide into 21 species.Further analyses suggested that the 9 strong am-ylase active strains belonged to 8 genera of 7 families ( Bacillaceae, Deinococcaceae, Microbacteriaceae, Micrococ-caceae, Nocardiaceae, Rhodobacteraceae, Xanthomonadaceae) in 4 phylogenetic groups ( Actinobacteria, Deinococ-cus-Thermus, Firmicutes, Proteobacteria):JSM 2215002 and JSM 2215134 belonged to the genus Paracoccus, JSM 2215012 Micrococcus, JSM 2215017 Arthrobacter, JSM 2215018 Microbacterium, JSM 2215076 Bacillus, JSM 2215104 Xanthomonas, JSM 2215109 Deinococcus, and JSM 2215126 Rhodococcus.The results showed that liquor fermentation workshop in Jiugui Liquor Co.Ltd.existed fairly high proportion of amylase producing strains and they highly had groups and species diversities.