微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
4期
54-61
,共8页
娄阳%张昭寰%肖莉莉%刘海泉%潘迎捷%赵勇
婁暘%張昭寰%肖莉莉%劉海泉%潘迎捷%趙勇
루양%장소환%초리리%류해천%반영첩%조용
抗生素抗性基因(ARGs)%聚合酶链式反应(PCR)%变性梯度凝胶电泳( DGGE)%污泥
抗生素抗性基因(ARGs)%聚閤酶鏈式反應(PCR)%變性梯度凝膠電泳( DGGE)%汙泥
항생소항성기인(ARGs)%취합매련식반응(PCR)%변성제도응효전영( DGGE)%오니
antibiotic resistance genes ( ARGs)%PCR%DGGE%sludge
探究新型环境污染物—抗生素抗性基因（ ARGs ）在校园环境中的分布状况。通过聚合酶链式反应（ PCR）对上海某高校使用5年新校区不同区域污水检查井污泥中8种四环素类、4种磺胺类、7种β－内酰胺类、4种链霉素类和5种氯霉素类ARGs进行定性研究，并利用变性梯度凝胶电泳（ DGGE）技术分析污泥中细菌群落的多样性。结果显示，校园各区域中共检出19种ARGs，有8种ARGs的检出率大于50％，其中磺胺类抗性基因sulI、sulII的检出率最高，为100％。实验区及餐饮区的ARGs检出种类最多，均为14种，其次为宿舍区（12种），教学区的ARGs检出最少（8种）。通过DGGE分析细菌群落结构，证明该地区的ARGs分布与细菌多样性无明显关系。新校区使用5年但ARGs污染严重，可能是由于人类活动（尤其是科研活动）对ARGs的产生及扩散存在促进作用。此外，细菌群落多样性与ARGs种类的关系表明ARGs在环境中的迁移可能受到除细菌种类之外其他环境因素的影响。
探究新型環境汙染物—抗生素抗性基因（ ARGs ）在校園環境中的分佈狀況。通過聚閤酶鏈式反應（ PCR）對上海某高校使用5年新校區不同區域汙水檢查井汙泥中8種四環素類、4種磺胺類、7種β－內酰胺類、4種鏈黴素類和5種氯黴素類ARGs進行定性研究，併利用變性梯度凝膠電泳（ DGGE）技術分析汙泥中細菌群落的多樣性。結果顯示，校園各區域中共檢齣19種ARGs，有8種ARGs的檢齣率大于50％，其中磺胺類抗性基因sulI、sulII的檢齣率最高，為100％。實驗區及餐飲區的ARGs檢齣種類最多，均為14種，其次為宿捨區（12種），教學區的ARGs檢齣最少（8種）。通過DGGE分析細菌群落結構，證明該地區的ARGs分佈與細菌多樣性無明顯關繫。新校區使用5年但ARGs汙染嚴重，可能是由于人類活動（尤其是科研活動）對ARGs的產生及擴散存在促進作用。此外，細菌群落多樣性與ARGs種類的關繫錶明ARGs在環境中的遷移可能受到除細菌種類之外其他環境因素的影響。
탐구신형배경오염물—항생소항성기인（ ARGs ）재교완배경중적분포상황。통과취합매련식반응（ PCR）대상해모고교사용5년신교구불동구역오수검사정오니중8충사배소류、4충광알류、7충β－내선알류、4충련매소류화5충록매소류ARGs진행정성연구，병이용변성제도응효전영（ DGGE）기술분석오니중세균군락적다양성。결과현시，교완각구역중공검출19충ARGs，유8충ARGs적검출솔대우50％，기중광알류항성기인sulI、sulII적검출솔최고，위100％。실험구급찬음구적ARGs검출충류최다，균위14충，기차위숙사구（12충），교학구적ARGs검출최소（8충）。통과DGGE분석세균군락결구，증명해지구적ARGs분포여세균다양성무명현관계。신교구사용5년단ARGs오염엄중，가능시유우인류활동（우기시과연활동）대ARGs적산생급확산존재촉진작용。차외，세균군락다양성여ARGs충류적관계표명ARGs재배경중적천이가능수도제세균충류지외기타배경인소적영향。
The purpose of this research was to investigate the distribution of antibiotic resistance genes ( ARGs) a new environmental pollutant in a university campus circumstances.Qualitative studies through PCR assays were con-ducted for ARGs encoding 8 categories of tetracycline, 4 sulfonamide, 5 chloramphenicol, 4 streptomycin, and 7 β-lactam from sludge collected in different wastewater checking wells in the only-5-year new university campus.Denatu-ring gradient gel electrophoresis ( DGGE) was used to analyze the diversity of bacterial community in the sludge.The results showed that 19 ARGs were detected in samples from every area in the campus, and the detection rates of more than 50%were as high as 8 categories.Among them the sulphonamide resistance genes sulI and sulII were the genes of the highest detection rate (100%).In experimental and dining areas, the largest number of ARGs (14) was de-tected, followed by 12 in dormitory area, and 8 ARGs were the least detected in teaching areas.DGGE analyses was done for the bacterial community structure and showed that there was no significant relationship between the distribu-tion of ARGs and the diversity of bacterial community.The new campus was set up only 5 years, however, the ARGs pollution was considerably serious.The appearance of ARGs may have been proliferated and promoted by human activ-ities;especially scientific research activities.Moreover, the relationship between bacterial diversity and ARGs catego-ries suggested that some other environmental factors beyond the bacterial categories may have affected the migration of ARGs in environment.
