CAJ | 학술논문

为了提高PHAMCL在门多萨假单胞菌NK－01中的积累，采用单因素实验和正交实验确立了发酵生产PHAMCL的最佳条件，即以PHA产量为指标的最佳发酵条件为15 g／L葡萄糖浓度、C／N＝50、发酵时间48 h，该条件下获得产量0．8 g／L以上的PHA；以PHA占菌体干重百分含量为指标的最佳发酵条件为10 g／L葡萄糖浓度、C／N＝60、发酵时间48 h，该条件下获得占菌体干重50％以上的PHA。该研究将为门多萨假单胞菌NK－01用于PHAMCL的规模化生产提供理论依据。
위료제고PHAMCL재문다살가단포균NK－01중적적루，채용단인소실험화정교실험학립료발효생산PHAMCL적최가조건，즉이PHA산량위지표적최가발효조건위15 g／L포도당농도、C／N＝50、발효시간48 h，해조건하획득산량0．8 g／L이상적PHA；이PHA점균체간중백분함량위지표적최가발효조건위10 g／L포도당농도、C／N＝60、발효시간48 h，해조건하획득점균체간중50％이상적PHA。해연구장위문다살가단포균NK－01용우PHAMCL적규모화생산제공이론의거。
In order to improve the accumulation of PHAMCL in vivo, single factor and orthogonal experiments were a-dopted to study the optimization fermentation conditions for Pseudomonas mendocina NK-01.When PHAMCL output was taken as an index, under optimized fermentation conditions at 15 g/L glucose, with C/N ratio at 50 and fermented for 48 h, produced over 0.8/L of PHAMCL .When the index was turned to PHAMCL percentage content of dried cell weight ( DCW) , the best fermentation conditions were 10 g/L glucose, with C/N ratio at 60 and fermented for 48 h, the PHAMCL output was over 50% of DCW.This study will provide theoretic foundations for large scale production of PHAMCL by P.mendocina NK-01.