微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
4期
37-42
,共6页
液体培养基配方%菌丝体生物量%胞外多糖产量%总产量%正交试验%桑黄
液體培養基配方%菌絲體生物量%胞外多糖產量%總產量%正交試驗%桑黃
액체배양기배방%균사체생물량%포외다당산량%총산량%정교시험%상황
liquid medium formula%mycelium biomass%exocellular polysaccharide yield%total yield%orthogonal test%Phellinus igniarius
研究液体培养基配方对桑黄菌丝体生物量和胞外多糖产量的影响。采用四因素三水平的正交试验方法。适宜于菌丝体生物量和胞外多糖产量的配方为A3 B1 C3 D2（麦麸10％，蛋白胨0．5％， KH2 PO40．35％， MgSO40．15％）， pH 6，菌丝体生物量和胞外多糖总产达到1．0077g／100 mL。优化的配方可以用于工业生产获得更多的菌丝体生物量和胞外多糖。
研究液體培養基配方對桑黃菌絲體生物量和胞外多糖產量的影響。採用四因素三水平的正交試驗方法。適宜于菌絲體生物量和胞外多糖產量的配方為A3 B1 C3 D2（麥麩10％，蛋白胨0．5％， KH2 PO40．35％， MgSO40．15％）， pH 6，菌絲體生物量和胞外多糖總產達到1．0077g／100 mL。優化的配方可以用于工業生產穫得更多的菌絲體生物量和胞外多糖。
연구액체배양기배방대상황균사체생물량화포외다당산량적영향。채용사인소삼수평적정교시험방법。괄의우균사체생물량화포외다당산량적배방위A3 B1 C3 D2（맥부10％，단백동0．5％， KH2 PO40．35％， MgSO40．15％）， pH 6，균사체생물량화포외다당총산체도1．0077g／100 mL。우화적배방가이용우공업생산획득경다적균사체생물량화포외다당。
The influence of liquid medium formula on biomass of Phellinus igniarius mycelium and the yield of exocel-lular polysaccharide were studied adopting four factors and three levels orthogonal experiment.The suitable formula for mycelium biomass and exocellular polysaccharide yield of P.igniarius was A3 B1 C3 D2 ( wheat bran 10%, peptone 0.5%, KH2PO4 0.35%, MgSO4 0.15%), pH 6.The total yield of mycelium biomass and exocellular polysaccharide yield was 1.007 7 g/100 mL.The optimized formula can be used in industrial setting in order to get more yield of P. igniarius mycelium biomass and exocellular polysaccharide.
