国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
International Journal of Traditional Chinese Medicine
2015年
9期
808-811
,共4页
四叶参多糖%酒精性肝损伤%小鼠%保护作用
四葉參多糖%酒精性肝損傷%小鼠%保護作用
사협삼다당%주정성간손상%소서%보호작용
Polysaccharide from Radix Codonopsis Lanceolatae%Alcoholic hepatic injury%Mice%Protective effect
目的 观察四叶参多糖对酒精致小鼠急性肝损伤的保护作用,并探讨其作用机制.方法 按随机数字表法将小鼠分为正常对照组,模型组,四叶参多糖高、中、低剂量组,每组10只.除正常对照组外,其余各组采用酒精灌胃法建立小鼠急性酒精性肝损伤模型.造模第1天开始给药,四叶参多糖高、中、低剂量组分别灌胃0.8、0.4、0.2 g/kg四叶参多糖溶液,正常对照组和模型组灌胃等体积蒸馏水.给药1次/d,连续10 d后,测定血清谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、三酰甘油(TG)水平,肝组织超氧化歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)水平,观察小鼠肝组织病理形态学变化.结果 与模型组比较,四叶参多糖高、中、低剂量组小鼠血清中ALT[(97.5±22.12)U/L、(108.8±22.15)U/L、(125.2±19.68)U/L比(152.3±17.32)U/L]、AST[(72.5±21.3)U/L、(89.2±17.47)U/L、(104.3±19.34)U/L比(1215±22.31)U/L]和 TG[(0.75±0.32)mmol/L、(0.80±0.22)mmol/L、(0.96±0.21)mmol/L 比(1.44± 0.23)mmol/L]水平降低(P<0.05);肝组织 SOD[(215.6 ± 22.68)U/mg、(203.2 ± 22.18)U/mg、(186.2 ± 18.34)U/mg比(164.3±17.54)U/mg]、GSH[(17.74±2.12)μmol/g、(14.84±1.12)μmol/g、(13.93±2.15)μmol/g比(12.23±2.09)μmol/g]水平升高(P<0.05),MDA[(6.22±2.12)nmol/mg、(6.22±2.12)nmol/mg、(8.36± 1.84)nmol/mg 比(9.35±1.68)nmol/mg]水平降低(P<0.05).结论 四叶参多糖可减少酒精致急性肝损伤小鼠氧化应激损伤,有较好的肝保护作用.
目的 觀察四葉參多糖對酒精緻小鼠急性肝損傷的保護作用,併探討其作用機製.方法 按隨機數字錶法將小鼠分為正常對照組,模型組,四葉參多糖高、中、低劑量組,每組10隻.除正常對照組外,其餘各組採用酒精灌胃法建立小鼠急性酒精性肝損傷模型.造模第1天開始給藥,四葉參多糖高、中、低劑量組分彆灌胃0.8、0.4、0.2 g/kg四葉參多糖溶液,正常對照組和模型組灌胃等體積蒸餾水.給藥1次/d,連續10 d後,測定血清穀氨痠轉氨酶(ALT)、天鼕氨痠轉氨酶(AST)、三酰甘油(TG)水平,肝組織超氧化歧化酶(SOD)、穀胱甘肽(GSH)、丙二醛(MDA)水平,觀察小鼠肝組織病理形態學變化.結果 與模型組比較,四葉參多糖高、中、低劑量組小鼠血清中ALT[(97.5±22.12)U/L、(108.8±22.15)U/L、(125.2±19.68)U/L比(152.3±17.32)U/L]、AST[(72.5±21.3)U/L、(89.2±17.47)U/L、(104.3±19.34)U/L比(1215±22.31)U/L]和 TG[(0.75±0.32)mmol/L、(0.80±0.22)mmol/L、(0.96±0.21)mmol/L 比(1.44± 0.23)mmol/L]水平降低(P<0.05);肝組織 SOD[(215.6 ± 22.68)U/mg、(203.2 ± 22.18)U/mg、(186.2 ± 18.34)U/mg比(164.3±17.54)U/mg]、GSH[(17.74±2.12)μmol/g、(14.84±1.12)μmol/g、(13.93±2.15)μmol/g比(12.23±2.09)μmol/g]水平升高(P<0.05),MDA[(6.22±2.12)nmol/mg、(6.22±2.12)nmol/mg、(8.36± 1.84)nmol/mg 比(9.35±1.68)nmol/mg]水平降低(P<0.05).結論 四葉參多糖可減少酒精緻急性肝損傷小鼠氧化應激損傷,有較好的肝保護作用.
목적 관찰사협삼다당대주정치소서급성간손상적보호작용,병탐토기작용궤제.방법 안수궤수자표법장소서분위정상대조조,모형조,사협삼다당고、중、저제량조,매조10지.제정상대조조외,기여각조채용주정관위법건립소서급성주정성간손상모형.조모제1천개시급약,사협삼다당고、중、저제량조분별관위0.8、0.4、0.2 g/kg사협삼다당용액,정상대조조화모형조관위등체적증류수.급약1차/d,련속10 d후,측정혈청곡안산전안매(ALT)、천동안산전안매(AST)、삼선감유(TG)수평,간조직초양화기화매(SOD)、곡광감태(GSH)、병이철(MDA)수평,관찰소서간조직병리형태학변화.결과 여모형조비교,사협삼다당고、중、저제량조소서혈청중ALT[(97.5±22.12)U/L、(108.8±22.15)U/L、(125.2±19.68)U/L비(152.3±17.32)U/L]、AST[(72.5±21.3)U/L、(89.2±17.47)U/L、(104.3±19.34)U/L비(1215±22.31)U/L]화 TG[(0.75±0.32)mmol/L、(0.80±0.22)mmol/L、(0.96±0.21)mmol/L 비(1.44± 0.23)mmol/L]수평강저(P<0.05);간조직 SOD[(215.6 ± 22.68)U/mg、(203.2 ± 22.18)U/mg、(186.2 ± 18.34)U/mg비(164.3±17.54)U/mg]、GSH[(17.74±2.12)μmol/g、(14.84±1.12)μmol/g、(13.93±2.15)μmol/g비(12.23±2.09)μmol/g]수평승고(P<0.05),MDA[(6.22±2.12)nmol/mg、(6.22±2.12)nmol/mg、(8.36± 1.84)nmol/mg 비(9.35±1.68)nmol/mg]수평강저(P<0.05).결론 사협삼다당가감소주정치급성간손상소서양화응격손상,유교호적간보호작용.
Objective To investigate the protective effects of polysaccharide from Radix Codonopsis Lanceolatae on acute alcoholic hepatic injury in mice.Methods The model of CCl4-induced acute hepatic injury was set up in mice, and then treated with polysaccharide from Radix Codonopsis Lanceolatae to observe the activities of ALT, AST and TG in serum and the levels of SOD, GSH and MDA in hepatic tissue and Observed the pathological changes in the liver of mice.Results Compared with model control group, the lance asiabell root polysaccharide liver injury in mice all dose groups in the activity of serum ALT(97.5 ± 22.12 U/L, 108.8 ± 22.15 U/L, 125.2 ± 19.68 U/Lvs. 152.3 ± 17.32 U/L), AST (72.5 ± 21.3 U/L, 89.2 ± 17.47 U/L, 104.3 ± 19.34 U/Lvs.1215 ± 22.31 U/L) and TG(0.75 ± 0.32 mmol/L, 0.80 ± 0.22 mmol/L, 0.96 ± 0.21 mmol/L vs.1.44 ± 0.23 mmol/L) reduce, can increase the liver damage the activity of SOD(215.6 ± 22.68 U/mg, 203.2 ± 22.18 U/mg, 186.2 ± 18.34 U/mgvs.164.3 ± 17.54 U/mg), GSH(17.74 ± 2.12μmol/g, 14.84 ± 1.12μmol/g, 13.93 ± 2.15μmol/gvs.12.23 ± 2.09μmol/g) in liver tissue of mice, decrease the activity of MDA(6.22 ± 2.12 nmol/mg, 6.22 ± 2.12 nmol/mg, 8.36 ± 1.84 nmol/mgvs.9.35 ± 1.68 nmol/mg) in liver tissue, showing significant differences (P<0.05), and there was certain dose dependent, and can improve the pathological injury of liver tissue.Conclusion Polysaccharide from Radix Codonopsis Lanceolatae had protective effects on acute liver induced by acute alcoholic hepatic injury in mice.
