CAJ | 학술논문

目的：：系统性研究经典Tgfβ信号通路各成员,在人类早期结直肠癌组织中,转录水平上的表达差异,以探索Tgfβ信号通路在人类结直肠癌发生中作用机制。方法：利用生物信息学手段,在GEO数据库中下载符合纳入标准的早期人类结直肠癌芯片结果,通过UltralEdit软件实现探针号和基因名的转换以建立全转录本表达数据表；通过KEGG信号通路图谱选择Tgfβ信号经典通路待测分子,统计学分析各待测分子在正常组癌症组中的表达差异。结果：在GEO数据库中下载和获得符合纳入标准的早期人类结直肠癌结直肠癌芯片12组基因芯片结果,对Tgfβ信号通路及其旁路共27个进行表达差异分析,其中表达水平上调的分子4个,下调的基因3个,具有或接近显著性意义。结论：在直肠癌发生过程中,Smads分子的表达下降阻断经典的Tgfβ信号的抗肿瘤作用,同时配体分子和受体分子的广泛上调可能刺激旁路分子的激活从而导致肿瘤的发生。
목적：：계통성연구경전Tgfβ신호통로각성원,재인류조기결직장암조직중,전록수평상적표체차이,이탐색Tgfβ신호통로재인류결직장암발생중작용궤제。방법：이용생물신식학수단,재GEO수거고중하재부합납입표준적조기인류결직장암심편결과,통과UltralEdit연건실현탐침호화기인명적전환이건립전전록본표체수거표；통과KEGG신호통로도보선택Tgfβ신호경전통로대측분자,통계학분석각대측분자재정상조암증조중적표체차이。결과：재GEO수거고중하재화획득부합납입표준적조기인류결직장암결직장암심편12조기인심편결과,대Tgfβ신호통로급기방로공27개진행표체차이분석,기중표체수평상조적분자4개,하조적기인3개,구유혹접근현저성의의。결론：재직장암발생과정중,Smads분자적표체하강조단경전적Tgfβ신호적항종류작용,동시배체분자화수체분자적엄범상조가능자격방로분자적격활종이도치종류적발생。
Objective: Systematic study of the transcriptional expression levels of canonical Tgfβ signaling pathways in early human colorectal cancer tissues, to shed light on molecular mechanism of canonical Tgfβ signaling underling the onset of human colorectal cancer. Methods: Using bioinformatic analysis, twelve sets of gene chip data that meet the criteria were downloaded from GEO database. By using UltraEdit software to convert the gene and probes name,to estab-lish gene expression corresponding names and sample data tables;using the KEGG pathway maps to inquiry the members of Tgfβsignaling pathway, using the Exel’ s TTEST function to calculate the sigifcance of each gene. Results:In the de-velopment process of the human colorectal cancer, there are twenty-seven genes involved in the Tgfβsignaling pathway of twelve sets of gene chip data, where 4 genes up-regulated and 3 down-regulated. Conclusion:During the colorectal tu-morigenesis, the decrease of Smads block the anti-cancer roles of canonical Tgfβ, and the increase of Tgfβ ligands and receptors induce the non-canonical pathway, which contribute to colorectal cancer progress.