中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
Chinese Journal of Woman and Child Health Research
2015年
4期
660-662,665
,共4页
吴晓康%马超锋%余鹏博%陈海龙%尹佳锋%杜毛绳%武海滨%邓慧玲
吳曉康%馬超鋒%餘鵬博%陳海龍%尹佳鋒%杜毛繩%武海濱%鄧慧玲
오효강%마초봉%여붕박%진해룡%윤가봉%두모승%무해빈%산혜령
手足口病%肠道菌群%变性梯度凝胶电泳%多样性%相似性
手足口病%腸道菌群%變性梯度凝膠電泳%多樣性%相似性
수족구병%장도균군%변성제도응효전영%다양성%상사성
hand-foot-mouth disease ( HFMD)%gut microbiota%denaturing gradient gel electrophoresis ( DGGE)%diversity%similarity
目的 探索手足口病患儿肠道菌群结构特征,了解肠道菌群与疾病的相关性. 方法 收集手足口病住院患儿32例和健康儿童14例粪便样本,提取肠道菌群总脱氧核糖核酸( DNA) ,16SrDNA-V3区聚合酶链反应( PCR)扩增,利用变性梯度凝胶电泳( DGGE)分子指纹图谱和测序技术,分析手足口病患儿肠道菌群组成结构、多样性、相似性等生态学特征. 结果 手足口病患儿与健康儿童相比,DGGE指纹图谱的条带数和Shannon-Wiener指数均显著降低(t=3.92,P<0.05;t=3.24,P<0.05),肠道菌群多样性降低;比较两组相似性系数和相似性系数累积曲线,手足口病患儿组内相似性降低(Z=-9.12,P<0.05),菌群结构更趋不同;优势条带切胶测序结果表明,手足口病患儿以厚壁菌门、变形菌门、拟杆菌门为主,肠道优势菌属可能发生变化.结论 手足口病患儿肠道菌群结构、组成发生变化,疾病的发生与肠道菌群具有一定的相关性.
目的 探索手足口病患兒腸道菌群結構特徵,瞭解腸道菌群與疾病的相關性. 方法 收集手足口病住院患兒32例和健康兒童14例糞便樣本,提取腸道菌群總脫氧覈糖覈痠( DNA) ,16SrDNA-V3區聚閤酶鏈反應( PCR)擴增,利用變性梯度凝膠電泳( DGGE)分子指紋圖譜和測序技術,分析手足口病患兒腸道菌群組成結構、多樣性、相似性等生態學特徵. 結果 手足口病患兒與健康兒童相比,DGGE指紋圖譜的條帶數和Shannon-Wiener指數均顯著降低(t=3.92,P<0.05;t=3.24,P<0.05),腸道菌群多樣性降低;比較兩組相似性繫數和相似性繫數纍積麯線,手足口病患兒組內相似性降低(Z=-9.12,P<0.05),菌群結構更趨不同;優勢條帶切膠測序結果錶明,手足口病患兒以厚壁菌門、變形菌門、擬桿菌門為主,腸道優勢菌屬可能髮生變化.結論 手足口病患兒腸道菌群結構、組成髮生變化,疾病的髮生與腸道菌群具有一定的相關性.
목적 탐색수족구병환인장도균군결구특정,료해장도균군여질병적상관성. 방법 수집수족구병주원환인32례화건강인동14례분편양본,제취장도균군총탈양핵당핵산( DNA) ,16SrDNA-V3구취합매련반응( PCR)확증,이용변성제도응효전영( DGGE)분자지문도보화측서기술,분석수족구병환인장도균군조성결구、다양성、상사성등생태학특정. 결과 수족구병환인여건강인동상비,DGGE지문도보적조대수화Shannon-Wiener지수균현저강저(t=3.92,P<0.05;t=3.24,P<0.05),장도균군다양성강저;비교량조상사성계수화상사성계수루적곡선,수족구병환인조내상사성강저(Z=-9.12,P<0.05),균군결구경추불동;우세조대절효측서결과표명,수족구병환인이후벽균문、변형균문、의간균문위주,장도우세균속가능발생변화.결론 수족구병환인장도균군결구、조성발생변화,질병적발생여장도균군구유일정적상관성.
Objective To explore the characteristics of gut microbiota in children with hand-foot-mouth disease ( HFMD) and investigate the correlation between gut microbiota and the disease.Methods Bacterial DNAs were extracted from the faecal samples of 32 patient children with HFMD and 14 healthy children, and PCR amplification was performed at 16SrDNA-V.Finger printing and sequencing of denaturing gradient gel electrophoresis ( DGGE) were used to analyze the composition, diversity and similarity of gut microbiota in patients with HFMD.Results Compared to healthy children, the number of DGGE bands and the Shannon-Wiener index of patients with HFMD were both significantly declined (t value was 3.92 and 3.24, respectively, both P<0.05), and the diversity of gut microbiota decreased. Comparison of the similarity coefficient and the accumulative distribution curves of dice similarity coefficient of both groups indicated that the similarity in HFMD group was declining (Z=-9.12,P<0.05), and the construction of gut microbiota was different.The results of splitting predominant gel bands and sequencing also revealed that Firmicutes, Proteobacteria, Bacteroidetes were mainly represented in patients with HFMD, and that the dominant bacterial genera might change.Conclusion The composition and construction of gut microbiota in patients with HFMD have changed, which suggests that the occurrence of the disease has the correlation with gut microbiota.
