CAJ | 학술논문

细菌性条斑病（简称细条病）是水稻的重要病害之一，随着气候的变暖，某些水稻品种有逐年发生加重的趋势，对产量影响较大。2013年浙江省金华市部分水稻种植区域发生细条病，发病严重的田块减产30％以上。采用组织分离法从发病水稻叶片分离获得6株细菌菌株，选择典型菌株JH01回接水稻幼苗，进行柯赫法则验证。接种后发病症状与自然发病症状一致，并重新分离得到此菌株，证明菌株JH01为水稻细条病的致病菌。通过形态学观察、常规生理生化指标测定、16S rDNA序列测定和同源性分析，鉴定菌株JH01为稻黄单胞菌水稻致病变种（ Xanthomonas oryzae pv．oryzicola ，Xoc）。
세균성조반병（간칭세조병）시수도적중요병해지일，수착기후적변난，모사수도품충유축년발생가중적추세，대산량영향교대。2013년절강성금화시부분수도충식구역발생세조병，발병엄중적전괴감산30％이상。채용조직분리법종발병수도협편분리획득6주세균균주，선택전형균주JH01회접수도유묘，진행가혁법칙험증。접충후발병증상여자연발병증상일치，병중신분리득도차균주，증명균주JH01위수도세조병적치병균。통과형태학관찰、상규생리생화지표측정、16S rDNA서렬측정화동원성분석，감정균주JH01위도황단포균수도치병변충（ Xanthomonas oryzae pv．oryzicola ，Xoc）。
Bacterial leaf streak ( BLS) is one of the most important diseases of rice, and as the climate warming; it has a worsening tendency to occur year by year that greatly affect the production.In 2013, BLS broke out in some rice planting areas of Jinhua City, Zhejiang Province, and caused decline over 30%of rice yield in some field with serious disease.Adopting the method of plant tissue pathogen isolation from pathogenic leaves, 6 bacterial strains were isola-ted and purified, the typical strain JH01 was selected to inoculate the rice seedlings to validate Koch ’ s law.The dis-ease symptoms of inoculation were consistent with the disease occurred in nature , and the same strain was rei-solated from the infected rice leaves.And proved that strain JH01 was the pathogen of rice BLS.The strain JH01 was charac-terized as Xanthomonas o ryzae pv.oryzicola by morphological observation, routine physiological and biochemical indi-ces tests, and 16S rDNA sequences and homology analyses.