激光生物学报
激光生物學報
격광생물학보
Acta Laser Biology Sinica
2015年
3期
257-262
,共6页
结直肠癌%TRAF4%Akt%己糖激酶II%糖酵解
結直腸癌%TRAF4%Akt%己糖激酶II%糖酵解
결직장암%TRAF4%Akt%기당격매II%당효해
Colon Cancer%TRAF4%Akt%Hexokinase II%Glycolysis
目的：：研究TRAF4影响结直肠癌细胞增殖的分子机制。方法：MTS和软琼脂集落形成实验检测基因沉默TRAF4后对结直肠癌细胞生长及相关信号通路的影响，检测TRAF4表达下调后结直肠癌细胞的糖酵解变化及己糖激酶II的表达和结直肠细胞对5-Fu的敏感性。结果：基因沉默TRAF4抑制结直肠癌细胞的停泊依赖和停泊非依赖增殖，抑制EGF诱导的Akt活化，下调结直肠癌细胞糖酵解，抑制己糖激酶II的表达，增加结直肠癌细胞对5-Fu的敏感性。结论：TRAF4通过调控糖代谢影响结直肠癌细胞增殖。
目的：：研究TRAF4影響結直腸癌細胞增殖的分子機製。方法：MTS和軟瓊脂集落形成實驗檢測基因沉默TRAF4後對結直腸癌細胞生長及相關信號通路的影響，檢測TRAF4錶達下調後結直腸癌細胞的糖酵解變化及己糖激酶II的錶達和結直腸細胞對5-Fu的敏感性。結果：基因沉默TRAF4抑製結直腸癌細胞的停泊依賴和停泊非依賴增殖，抑製EGF誘導的Akt活化，下調結直腸癌細胞糖酵解，抑製己糖激酶II的錶達，增加結直腸癌細胞對5-Fu的敏感性。結論：TRAF4通過調控糖代謝影響結直腸癌細胞增殖。
목적：：연구TRAF4영향결직장암세포증식적분자궤제。방법：MTS화연경지집락형성실험검측기인침묵TRAF4후대결직장암세포생장급상관신호통로적영향，검측TRAF4표체하조후결직장암세포적당효해변화급기당격매II적표체화결직장세포대5-Fu적민감성。결과：기인침묵TRAF4억제결직장암세포적정박의뢰화정박비의뢰증식，억제EGF유도적Akt활화，하조결직장암세포당효해，억제기당격매II적표체，증가결직장암세포대5-Fu적민감성。결론：TRAF4통과조공당대사영향결직장암세포증식。
Objective: To investigate the mechanism of TRAF4 mediated colon cancer cells proliferation. Methods:Colon cancer cells proliferation were tested by MTS and soft agar assayin sh-Mock and sh-TRAF4 stable cell lines. West-ern blot was conducted to detect the signaling transduction and the expression of Hexokinase II. Then the levels of glycol-ysis were assessed via measurement of glucose consumption and lactate production, respectively. The sensitivity of these stable cells to 5-Fu was detected by Western blotting. Results:Knockdown TRAF4 attenuates human colon cancer cells anchorage-dependent and -independent growth, TRAF4 knocking down inhibits EGF-induced Akt activation, as well as glycolysis and Hexokinase II expression. Moreover, knockdown TRAF4 increases the sensitivity of colon cancer cells to 5-Fu treatment. Conclusion: TRAF4 affects human colon cancer cells proliferation via glycolysis regulation.
