CAJ | 학술논문

采用响应面分析法（ RSM）对胶质类芽胞杆菌的初级种子培养基进行了优化，以提高其菌体密度和后续发酵效率。首先采用单因子试验筛选出提升菌体密度的适宜碳源和氮源，分别为麦芽糖和胰蛋白胨，在此基础上采用Plackett-Burman（ PB）试验设计法，对9种影响菌体生长繁殖的因素进行了评价，结果表明，麦芽糖和MgSO4·7H2 O对菌体繁殖影响最为显著。用快速登高试验逼近关键因素的最大响应区域，通过中心组合试验和验证试验，获得胶质类芽胞杆菌优化培养基成分为麦芽糖2．5 g／L，胰蛋白胨1.0 g／L，MgSO4·7H2 O 0.73 g／L，K2 HPO4·3H2 O 0．4 g／L，NaCl 0．06 g／L，FeCl30．6 mg／L，水杨酸10 mg／L，CaCO31．0 g／L。使用该优化培养基可将菌体密度较优化前提高了近10倍，含量达到4．12×108 cfu／mL。结果为提高胶质类芽胞杆菌的生产发酵水平和保证产品质量提供依据。
채용향응면분석법（ RSM）대효질류아포간균적초급충자배양기진행료우화，이제고기균체밀도화후속발효효솔。수선채용단인자시험사선출제승균체밀도적괄의탄원화담원，분별위맥아당화이단백동，재차기출상채용Plackett-Burman（ PB）시험설계법，대9충영향균체생장번식적인소진행료평개，결과표명，맥아당화MgSO4·7H2 O대균체번식영향최위현저。용쾌속등고시험핍근관건인소적최대향응구역，통과중심조합시험화험증시험，획득효질류아포간균우화배양기성분위맥아당2．5 g／L，이단백동1.0 g／L，MgSO4·7H2 O 0.73 g／L，K2 HPO4·3H2 O 0．4 g／L，NaCl 0．06 g／L，FeCl30．6 mg／L，수양산10 mg／L，CaCO31．0 g／L。사용해우화배양기가장균체밀도교우화전제고료근10배，함량체도4．12×108 cfu／mL。결과위제고효질류아포간균적생산발효수평화보증산품질량제공의거。
In order to improve thallus density and the follow-up fermentation efficiency of Paenibacillus mucilagino-sus, response surface methodology ( RSM) was used to optimize the preliminary seed medium.Firstly single factor ex-periments were adopted to screen carbon and nitrogen sources befitting for the promotion of thallus density, they were maltose and tryptone respectively.Based on these Plackett-Burman ( PB) experiment designing method was adopted to evaluate nine factors that affect the growth and propagation of thallus; the results showed that maltose and MgSO4 · 7H2 O affected the most significant to the thallus propagation.After that, fast ascending experiment was used to ap-proach the largest response region of the key factors, and obtained the optimum medium components for P.mucilagi-nosus through central combination experiment and verification experiment they were:maltose 2.5 g/L, tryptone 1.0 g/L, MgSO4 · 7H2 O 0.73 g/L, K2 HPO4 · 3H2 O 0.4 g/L, NaCl 0.06 g/L, FeCl3 0.6 mg/L, salicylic acid 10 mg/L, CaCO3 1.0 g/L.With the optimized medium, the thallus density increased nearly 10 times as compared with that be-fore the optimization, the content reached up to 4.12 ×108 cfu/mL.The results can provide a basis for improving the fermentation level of P.mucilaginosus and ensuring the quality of the products.