CAJ | 학술논문

目的：：评价光动力疗法( PDT)联合声动力学疗法( SDT)对小鼠鳞癌超微结构的影响。方法：以镓卟呤衍生物ATX-70作为光敏剂和声敏剂，分别用光动力疗法、声动力疗法以及两者联合应用处理小鼠鳞癌，利用透射电镜观察不同时间段取材的细胞超微结构的变化。结果：激光或超声激活ATX-70对鳞癌细胞超微结构的破坏程度随取材时间的延长而加剧，联合应用对肿瘤细胞破坏程度更明显。损伤位点主要集中在胞膜、线粒体、内质网及细胞核上，同时还观察到一些肿瘤细胞表现出明显的凋亡特征。结论：光动力学结合声动力学疗法比单用肿瘤细胞破坏程度更明显，主要通过破坏细胞超微结构杀伤肿瘤细胞，部分通过诱导凋亡杀伤。
목적：：평개광동력요법( PDT)연합성동역학요법( SDT)대소서린암초미결구적영향。방법：이가계령연생물ATX-70작위광민제화성민제，분별용광동력요법、성동력요법이급량자연합응용처리소서린암，이용투사전경관찰불동시간단취재적세포초미결구적변화。결과：격광혹초성격활ATX-70대린암세포초미결구적파배정도수취재시간적연장이가극，연합응용대종류세포파배정도경명현。손상위점주요집중재포막、선립체、내질망급세포핵상，동시환관찰도일사종류세포표현출명현적조망특정。결론：광동역학결합성동역학요법비단용종류세포파배정도경명현，주요통과파배세포초미결구살상종류세포，부분통과유도조망살상。
Objective: To observe the ultrastucture of experimental squamous cell carcinoma in mice after sono-photo-dynamic combination therapy. Methods:Analogues of a gallium-porphyrin derivative (ATX-70) was used as photosen-sitizer and sonosensitizer in this study. The squamous cell carcinoma in mice was treated by photodynamic therapy, son-odynamic therapy and sono-photodynamic combination therapy respectively. The changes of ultrastructure of sample preparation for different time were observed by transmission electron microscope. Results: The degree of destruction of treated squamous cell carcinoma cells was enhanced with the increasing of time, which was induced by photo-activated or ultrasound-activated ATX-70. However, the sono-photodynamic combination therapy was more effective. The sites destroyed mainly involved cell membrane, mitochondrion, endoplasmic reticulum and cell nuclei. Furthermore, morpho-iogical characters of apoptosis were observed on cancer cells. Conclusion: This study suggested that the sono-photody-namic combination therapy is more effective in terms of its antitumor effect when compared with photodynamic therapy or sonodynamic therapy alone. The killing of tumor cells was ascribed mainly to the damage of ultrastructure, apoptosis wasalso induced during the killing process.