微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
4期
106-109
,共4页
梁慧嘉%焦春伟%杨咏善%张命龙%谢意珍
樑慧嘉%焦春偉%楊詠善%張命龍%謝意珍
량혜가%초춘위%양영선%장명룡%사의진
食用菌%水提物%DPPH自由基%羟基自由基%超氧阴离子自由基
食用菌%水提物%DPPH自由基%羥基自由基%超氧陰離子自由基
식용균%수제물%DPPH자유기%간기자유기%초양음리자자유기
mushroom%water extract%DPPH free radical%hydroxyl free radical%superoxide anion free radical
用DPPH自由基清除法、羟基自由基清除法和超氧阴离子自由基清除法对4种珍稀食用菌灵芝、云芝、茶树菇、松茸的水提物进行抗氧化活性评价,为更好评价其抗氧化活性,以维生素C作为阳性对照。实验结果显示:4种食用菌水提物具有不同程度的抗氧化活性。云芝对DPPH自由基的清除能力最强,其IC50值为1.46 mg/mL,维生素C清除DPPH自由基的IC50为0.046 mg/mL;茶树菇对清除羟基自由基的清除能力最强,其IC50值为1.41 mg/mL,云芝和松茸也有较强清除羟自由基能力,其IC50值分别为1.56、1.57 mg/mL,三者的清除能力均明显优于阳性对照样品,维生素C清除羟自由基的IC50为2.41 mg/mL;灵芝和云芝有较强清除超氧阴离子自由基能力,其IC50值分别为124.48、138.28 mg/mL。
用DPPH自由基清除法、羥基自由基清除法和超氧陰離子自由基清除法對4種珍稀食用菌靈芝、雲芝、茶樹菇、鬆茸的水提物進行抗氧化活性評價,為更好評價其抗氧化活性,以維生素C作為暘性對照。實驗結果顯示:4種食用菌水提物具有不同程度的抗氧化活性。雲芝對DPPH自由基的清除能力最彊,其IC50值為1.46 mg/mL,維生素C清除DPPH自由基的IC50為0.046 mg/mL;茶樹菇對清除羥基自由基的清除能力最彊,其IC50值為1.41 mg/mL,雲芝和鬆茸也有較彊清除羥自由基能力,其IC50值分彆為1.56、1.57 mg/mL,三者的清除能力均明顯優于暘性對照樣品,維生素C清除羥自由基的IC50為2.41 mg/mL;靈芝和雲芝有較彊清除超氧陰離子自由基能力,其IC50值分彆為124.48、138.28 mg/mL。
용DPPH자유기청제법、간기자유기청제법화초양음리자자유기청제법대4충진희식용균령지、운지、다수고、송용적수제물진행항양화활성평개,위경호평개기항양화활성,이유생소C작위양성대조。실험결과현시:4충식용균수제물구유불동정도적항양화활성。운지대DPPH자유기적청제능력최강,기IC50치위1.46 mg/mL,유생소C청제DPPH자유기적IC50위0.046 mg/mL;다수고대청제간기자유기적청제능력최강,기IC50치위1.41 mg/mL,운지화송용야유교강청제간자유기능력,기IC50치분별위1.56、1.57 mg/mL,삼자적청제능력균명현우우양성대조양품,유생소C청제간자유기적IC50위2.41 mg/mL;령지화운지유교강청제초양음리자자유기능력,기IC50치분별위124.48、138.28 mg/mL。
Antioxidant activities in vitro of polysaccharides from Ganoderma lucidum, Coriolus versicolor, Agrocybe cy-lindracea and Matsutake mushroom, and vitamin C as a positive control.The anti-oxidation of polysaccharides was evaluated by DPPH radical scavenging activities, hydroxyl free radical scavenging activities and superoxide anion scav-enging activities.The results showed that four different polysaccharides had certain abilities of antioxidant activities. C.versicolor showed the most potent capacity for scavenging DPPH free radical, IC50 values reaching 1.46 mg/mL, the IC50 values of vitamin C is 0.046 mg/mL;A.cylindracea showed the most potent capacity for scavenging hydroxyl free radical, IC50 values reaching 1.41 mg/mL, the IC50 values of Vitamin C is 2.41 mg/mL;C.versicolor and Mat-sutake mushroom also had good scavenging activities, IC50 values reaching 1.56 mg/mL, 1.57 mg/mL, respectively. They were better scavenging activities than positive control.G.lucidum and C.versicolor also had good superoxide an-ion scavenging activities, IC50 values reaching 124.48 mg/mL, 138.28 mg/mL.