CAJ | 학술논문

目的：探讨不同浓度米非司酮对人子宫内膜癌HEC－1－B细胞株的增殖抑制作用。方法对人子宫内膜癌HEC－1－B细胞株进行体外培养，细胞呈指数增长期时用不同浓度（0，0.1，1，10和100μmol· L－1）的米非司酮对细胞株进行处理，并继续培养观察96 h，用四甲基偶氮唑蓝比色法检测不同浓度米非司酮作用下，不同时点细胞增殖能力。用免疫印迹法检测0，1，10μmol· L－1米非司酮作用48 h后Ki－67和C－myc蛋白表达水平。结果不同浓度的米非司酮对人子宫内膜癌HEC－1－B细胞株均有一定的增殖抑制作用，且随浓度的增加，作用时间的延长，抑制作用明显增强（ P＜0.05）。不同浓度米非司酮可抑制Ki－67和C－myc蛋白表达，且成剂量依赖性（ P＜0.05）。结论米非司酮可能通过Ki－67和C－myc通路抑制人子宫内膜癌HEC－1－B细胞株的增殖作用。
목적：탐토불동농도미비사동대인자궁내막암HEC－1－B세포주적증식억제작용。방법대인자궁내막암HEC－1－B세포주진행체외배양，세포정지수증장기시용불동농도（0，0.1，1，10화100μmol· L－1）적미비사동대세포주진행처리，병계속배양관찰96 h，용사갑기우담서람비색법검측불동농도미비사동작용하，불동시점세포증식능력。용면역인적법검측0，1，10μmol· L－1미비사동작용48 h후Ki－67화C－myc단백표체수평。결과불동농도적미비사동대인자궁내막암HEC－1－B세포주균유일정적증식억제작용，차수농도적증가，작용시간적연장，억제작용명현증강（ P＜0.05）。불동농도미비사동가억제Ki－67화C－myc단백표체，차성제량의뢰성（ P＜0.05）。결론미비사동가능통과Ki－67화C－myc통로억제인자궁내막암HEC－1－B세포주적증식작용。
Objective To evaluate the effects of mifepristone on the pro-liferation of human endometrial carcinoma HEC-1-B cellline in vitro.Methods The human endometrial carcinoma HEC-1-B cell line were cultured and treated with different concentrations of mifepristone (0,0.1, 1 , 10 , 100 μmol · L-1 ) for 96 hours.The cell proliferation ability at different time points were tested by MTT array.And the Ki-67 protein and C -myc protein expression for human endometrial carcinoma HEC-1-B cell line treated with mifepristone were arrayed after 48 hours.Results The mifepristone of different concentration can inhibit the proliferation of human endometrial carcinoma HEC-1-B cellline in vitro ( P <0.05 ).With the increase of concentration, the inhibition effects grew significantly.Different concentrations of mifepristone can inhibit the Ki-67 protein and C-myc protein expression after 48 h with dose-dependent effects ( P<0.05 ).Conclusion Mifepristone inhibit the proliferation of human endometrial carcinoma HEC-1-B cell line in vitro through Ki-67 and C-myc pathway.