中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
The Chinese Journal of Clinical Pharmacology
2015年
15期
1519-1521
,共3页
来氟米特%实验性变态反应性脑脊髓炎%多发性硬化
來氟米特%實驗性變態反應性腦脊髓炎%多髮性硬化
래불미특%실험성변태반응성뇌척수염%다발성경화
leflunomide%experimental allergic encephalomyelitis%multiple sclerosis
目的：研究来氟米特对实验性变态反应性脑脊髓炎（ EAE）的保护作用并探讨来氟米特的作用机制。方法将50只成年雌性豚鼠随机分为5组，各10只，分别为正常组、模型组与高中低3个剂量实验组。实验组分别给予来氟米特40，20，10 mg· kg－1· d－1（均3 mL），模型组和正常组均灌骨等量生理盐水。EAE诱导后，观察各组EAE发病率、潜伏期时间及神经功能障碍评分，用酶联免疫吸附法检测各组外周血白细胞介素－4（ IL－4）及γ干扰素（ IFN－γ）含量。结果与模型组及中、低2个剂量实验组相比较，高剂量实验组EAE发病率最低为40％（4／10），潜伏期时间最长为（14.8±3.8）d，神经功能障碍评分最低为（1.7±0.5）分（均P＜0.05）。模型组，IL－4水平低于正常组，而IFN－γ高于正常组。与模型组比较，各实验组的IL－4水平明显增高，而IFN－γ明显降低，且呈剂量依赖性（均P＜0.05）。结论来氟米特可降低EAE豚鼠发病率，减轻高峰期临床症状，对EAE大鼠发病具有保护作用。
目的：研究來氟米特對實驗性變態反應性腦脊髓炎（ EAE）的保護作用併探討來氟米特的作用機製。方法將50隻成年雌性豚鼠隨機分為5組，各10隻，分彆為正常組、模型組與高中低3箇劑量實驗組。實驗組分彆給予來氟米特40，20，10 mg· kg－1· d－1（均3 mL），模型組和正常組均灌骨等量生理鹽水。EAE誘導後，觀察各組EAE髮病率、潛伏期時間及神經功能障礙評分，用酶聯免疫吸附法檢測各組外週血白細胞介素－4（ IL－4）及γ榦擾素（ IFN－γ）含量。結果與模型組及中、低2箇劑量實驗組相比較，高劑量實驗組EAE髮病率最低為40％（4／10），潛伏期時間最長為（14.8±3.8）d，神經功能障礙評分最低為（1.7±0.5）分（均P＜0.05）。模型組，IL－4水平低于正常組，而IFN－γ高于正常組。與模型組比較，各實驗組的IL－4水平明顯增高，而IFN－γ明顯降低，且呈劑量依賴性（均P＜0.05）。結論來氟米特可降低EAE豚鼠髮病率，減輕高峰期臨床癥狀，對EAE大鼠髮病具有保護作用。
목적：연구래불미특대실험성변태반응성뇌척수염（ EAE）적보호작용병탐토래불미특적작용궤제。방법장50지성년자성돈서수궤분위5조，각10지，분별위정상조、모형조여고중저3개제량실험조。실험조분별급여래불미특40，20，10 mg· kg－1· d－1（균3 mL），모형조화정상조균관골등량생리염수。EAE유도후，관찰각조EAE발병솔、잠복기시간급신경공능장애평분，용매련면역흡부법검측각조외주혈백세포개소－4（ IL－4）급γ간우소（ IFN－γ）함량。결과여모형조급중、저2개제량실험조상비교，고제량실험조EAE발병솔최저위40％（4／10），잠복기시간최장위（14.8±3.8）d，신경공능장애평분최저위（1.7±0.5）분（균P＜0.05）。모형조，IL－4수평저우정상조，이IFN－γ고우정상조。여모형조비교，각실험조적IL－4수평명현증고，이IFN－γ명현강저，차정제량의뢰성（균P＜0.05）。결론래불미특가강저EAE돈서발병솔，감경고봉기림상증상，대EAE대서발병구유보호작용。
Objective To study the protective effect and mechanism of leflunomide in Guinea pig with experimental allergic encephalomyelitis ( EAE).Methods Fifty adult female Guinea pigs were randomly divi ded into five groups: normal group, model group, and high dose, mo derate dose,low dose test groups, ten cases each group.The high, mode-rate and low dose groups were given to 40, 20,10 mg· kg -1· d-1(all 3 mL ) of leflunomide, respectively.The model group and normal group took the equal quantity of normal saline.After the induction of EAE, the incidence rate, latency time and neurological dysfunction score of each group was observed.The content of interleukin-4 ( IL-4 ) and interfer-on gamma ( IFN-γ) was detected by enzyme-linked immuno sorbent assay ( ELISA).Results Compared with the model group, moderate dose and low dose group, the high dose group had the lowest incidence rate of EAE (40%, 4/10) and longest latency time (14.8 ±3.8) d and lowest nerve dysfunction score (1.7 ±0.5) (all P<0.05).The level of IL-4 of model group was lower than that of normal group while the level of IFN-γof model group was higher than that of normal group.The level of IL-4 of each test group was higher than that of model group, while the level of IFN-γwas lower than that of model group.It was dose dependent present (P<0.05).Conclusion Leflunomide can reduce the incidence rate of EAE in Guinea pigs, re-lieve the clinical symptoms at peak time and have a protective effect in the attack of EAE.
