微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
4期
62-66
,共5页
鲍曼不动杆菌%多重耐药性%β-内酰胺酶%耐药基因
鮑曼不動桿菌%多重耐藥性%β-內酰胺酶%耐藥基因
포만불동간균%다중내약성%β-내선알매%내약기인
Acinetobacter baumannii%multiple drug resistance%β-lactamase%resistance genes
研究多重耐药鲍曼不动杆菌的耐药性及β－内酰胺酶耐药基因的携带情况。采用VIKET Compact 2全自动细菌鉴定系统进行细菌鉴定，采用纸片扩散法（K－B法）测定鲍曼不动杆菌对抗菌药物的耐药性，应用聚合酶链反应（ PCR）法检测β－内酰胺酶耐药基因。32株多重耐药鲍曼不动杆菌对13种常用抗菌药物的耐药率均＞80％，对亚胺培南和美罗培南耐药率分别高达78．1％和71．9％，头孢哌酮／舒巴坦耐药率31．3％，多粘菌素B抗菌活性最好，耐药率0％。检出超广谱β－内酰胺酶（ ESBLs）和头孢菌素酶（ AmpC）耐药基因，未检出金属β－内酰胺酶（ MBLs）耐药基因。32株多重耐药鲍曼不动杆菌TEM基因均阳性，17株检出PER基因，29株检出ADC基因。有16株菌同时携带TEM、PER、ADC基因。结果表明，同时携带TEM、PER、ADC基因是安徽医科大学解放军174临床学院鲍曼不动杆菌产生多重耐药性的原因之一。
研究多重耐藥鮑曼不動桿菌的耐藥性及β－內酰胺酶耐藥基因的攜帶情況。採用VIKET Compact 2全自動細菌鑒定繫統進行細菌鑒定，採用紙片擴散法（K－B法）測定鮑曼不動桿菌對抗菌藥物的耐藥性，應用聚閤酶鏈反應（ PCR）法檢測β－內酰胺酶耐藥基因。32株多重耐藥鮑曼不動桿菌對13種常用抗菌藥物的耐藥率均＞80％，對亞胺培南和美囉培南耐藥率分彆高達78．1％和71．9％，頭孢哌酮／舒巴坦耐藥率31．3％，多粘菌素B抗菌活性最好，耐藥率0％。檢齣超廣譜β－內酰胺酶（ ESBLs）和頭孢菌素酶（ AmpC）耐藥基因，未檢齣金屬β－內酰胺酶（ MBLs）耐藥基因。32株多重耐藥鮑曼不動桿菌TEM基因均暘性，17株檢齣PER基因，29株檢齣ADC基因。有16株菌同時攜帶TEM、PER、ADC基因。結果錶明，同時攜帶TEM、PER、ADC基因是安徽醫科大學解放軍174臨床學院鮑曼不動桿菌產生多重耐藥性的原因之一。
연구다중내약포만불동간균적내약성급β－내선알매내약기인적휴대정황。채용VIKET Compact 2전자동세균감정계통진행세균감정，채용지편확산법（K－B법）측정포만불동간균대항균약물적내약성，응용취합매련반응（ PCR）법검측β－내선알매내약기인。32주다중내약포만불동간균대13충상용항균약물적내약솔균＞80％，대아알배남화미라배남내약솔분별고체78．1％화71．9％，두포고동／서파탄내약솔31．3％，다점균소B항균활성최호，내약솔0％。검출초엄보β－내선알매（ ESBLs）화두포균소매（ AmpC）내약기인，미검출금속β－내선알매（ MBLs）내약기인。32주다중내약포만불동간균TEM기인균양성，17주검출PER기인，29주검출ADC기인。유16주균동시휴대TEM、PER、ADC기인。결과표명，동시휴대TEM、PER、ADC기인시안휘의과대학해방군174림상학원포만불동간균산생다중내약성적원인지일。
The carrying situation of the resistance and the resistant genes of β-lactamases in multiple drug resistant Acinetobacter baumannii ( MDR-AB) was studied.The bacterial identification were performed by VITEK 2 compact full-automatic bacterial identification system.Paper diskette diffusion method ( K-B method) was used to detect the drug resistance of A.baumannii to antibiotic drugs.Theβ-lactamase resistance genes were amplified by PCR.The re-sults showed that drug resistance rates of 32 stains of MDR-AB to 13 kinds of commonly used antibiotic drugs were all over 80%.The resistance rates of MDR-AB to imipenem and meropenem were as high as 78.1% and 71.9%, re-spectively, to cefoperazone/sulbactam was 31.3%.Polymyxin B performed the best antibiotic activity, resistance rates was 0%.The resistance genes of extrabroad-spectrum β-lactamases ( ESBLs) and cephalosporinase ( AmpC) were detected, but non metal β-lactamase (MBLs) resistance genes was detected.Of 32 strains of MDR-AB, TEM genes were positive in all strains, 17 strains detected PER gene, 29 strains detected ADC genes.16 strains simultane-ously carry TEM, PER, ADC genes.In conclusion, simultaneously carrying TEM, PER, ADC genes may have been one reason that caused A.baumannii multi-drug resistance.
