临床儿科杂志
臨床兒科雜誌
림상인과잡지
Journal of Clinical Pediatrics
2015年
9期
817-821
,共5页
甘燕子%郝锦丽%曾其毅%杨鹏
甘燕子%郝錦麗%曾其毅%楊鵬
감연자%학금려%증기의%양붕
高氧%肺上皮细胞%肺表面活性蛋白%大鼠
高氧%肺上皮細胞%肺錶麵活性蛋白%大鼠
고양%폐상피세포%폐표면활성단백%대서
hyperoxia%alveolar epithelial cells%surfactant protein%rat
目的:探讨高浓度氧致新生鼠肺损伤时肺表面活性蛋白C、D(SP-C、SP-D)以及肺泡上皮细胞凋亡率的变化。方法生后24 h内的新生大鼠,随机分为空气组和高氧组;空气组常规饲养,高氧组置常压高氧箱内吸入浓度为90%的氧;两组分别于实验第1、3、7、10、14天,取8只新生鼠的肺组织进行苏木精-伊红(HE)染色并观察其病理变化,采用末端标记法检测肺上皮细胞凋亡率。采用酶联免疫法检测支气管肺泡灌洗液(BALF)中SP-C、SP-D。结果空气组新生鼠随日龄增加肺泡逐渐形成,形态规则,大小均匀;高氧组新生鼠随日龄增加肺泡数量逐渐减少,小血管扩张,出血增多,间质细胞增多,肺组织水肿。空气组新生大鼠BALF中的SP-C随日龄增长逐渐降低;高氧组第1天SP-C低于空气组,第3天SP- C高于空气组,第7天达高峰,第10天SP-C的含量开始下降,第14天下降更加明显。空气组SP-D的含量随日龄的增长亦逐渐降低;高氧组第1天SP-D的含量与空气组无明显差异,第3天SP-D的含量开始增加,第7天达到峰值,第10天SP-D的含量开始下降,第14天下降显著。结论长期吸入高浓度氧抑制肺泡发育,随吸入高浓度氧时间的延长,肺上皮细胞凋亡率增加,肺组织中SP-C、SP-D先增高后下降。
目的:探討高濃度氧緻新生鼠肺損傷時肺錶麵活性蛋白C、D(SP-C、SP-D)以及肺泡上皮細胞凋亡率的變化。方法生後24 h內的新生大鼠,隨機分為空氣組和高氧組;空氣組常規飼養,高氧組置常壓高氧箱內吸入濃度為90%的氧;兩組分彆于實驗第1、3、7、10、14天,取8隻新生鼠的肺組織進行囌木精-伊紅(HE)染色併觀察其病理變化,採用末耑標記法檢測肺上皮細胞凋亡率。採用酶聯免疫法檢測支氣管肺泡灌洗液(BALF)中SP-C、SP-D。結果空氣組新生鼠隨日齡增加肺泡逐漸形成,形態規則,大小均勻;高氧組新生鼠隨日齡增加肺泡數量逐漸減少,小血管擴張,齣血增多,間質細胞增多,肺組織水腫。空氣組新生大鼠BALF中的SP-C隨日齡增長逐漸降低;高氧組第1天SP-C低于空氣組,第3天SP- C高于空氣組,第7天達高峰,第10天SP-C的含量開始下降,第14天下降更加明顯。空氣組SP-D的含量隨日齡的增長亦逐漸降低;高氧組第1天SP-D的含量與空氣組無明顯差異,第3天SP-D的含量開始增加,第7天達到峰值,第10天SP-D的含量開始下降,第14天下降顯著。結論長期吸入高濃度氧抑製肺泡髮育,隨吸入高濃度氧時間的延長,肺上皮細胞凋亡率增加,肺組織中SP-C、SP-D先增高後下降。
목적:탐토고농도양치신생서폐손상시폐표면활성단백C、D(SP-C、SP-D)이급폐포상피세포조망솔적변화。방법생후24 h내적신생대서,수궤분위공기조화고양조;공기조상규사양,고양조치상압고양상내흡입농도위90%적양;량조분별우실험제1、3、7、10、14천,취8지신생서적폐조직진행소목정-이홍(HE)염색병관찰기병리변화,채용말단표기법검측폐상피세포조망솔。채용매련면역법검측지기관폐포관세액(BALF)중SP-C、SP-D。결과공기조신생서수일령증가폐포축점형성,형태규칙,대소균균;고양조신생서수일령증가폐포수량축점감소,소혈관확장,출혈증다,간질세포증다,폐조직수종。공기조신생대서BALF중적SP-C수일령증장축점강저;고양조제1천SP-C저우공기조,제3천SP- C고우공기조,제7천체고봉,제10천SP-C적함량개시하강,제14천하강경가명현。공기조SP-D적함량수일령적증장역축점강저;고양조제1천SP-D적함량여공기조무명현차이,제3천SP-D적함량개시증가,제7천체도봉치,제10천SP-D적함량개시하강,제14천하강현저。결론장기흡입고농도양억제폐포발육,수흡입고농도양시간적연장,폐상피세포조망솔증가,폐조직중SP-C、SP-D선증고후하강。
ObjectiveTo study the change of the pulmonary surfactant protein C, D (SP-C, SP-D) and apoptosis of alveolar epithelium cells in neonatal rats with hyperoxia-induced lung injury.MethodsThe neonatal rats born within 24 hours were divided into the air group (n=50) and the hyperoxia group (n=50). The lung tissue was collected on the ifrst, third, seventh, tenth, fourteenth day after the hyperoxia exposure. The pathological changes were observed by HE staining. The apoptosis rate of lung epithelial cells was detected by TUNEL (terminal deoxyn ucleotidyl transfer-mediated end labeling). The content of SP-C and SP-D in broncho alveolar lavage lfuid (BALF) was detected by enzyme-linked immunosorbent assay.ResultsIn the air group, as age increased, the alveolar were gradually more completely formed with the regular shape and uniform size. Mean-while, in the hyperoxia group, as age increased, the number of alveolar was reduced, the small blood vessels expanded, the alve-olar hemorrhage was increased, the interstitial cells were increased and the lung tissue was swelling. The levels of SP-C, SP-D decreased with the increase of age in the air group. The level of SP-C in hyperoxia group was lower than that in the air group on the ifrst day. It was higher than that in the air group on the third day, peaked on the seventh day, and then it began to decline on the tenth day and decreased more obviously on the fourteenth day. The level of SP-D in hyperoxia group was not signiifcantly dif-ferent from that in the air group on the ifrst day, was higher than that in the air group on the third day and peaked on the seventh day. Then it began to decline on the tenth day and decreased more on the fourteenth day. ConclusionsLong-term inhalation of high concentrations of oxygen inhibits alveolar development. With the prolonged time of oxygen inhalation, the apoptosis of lung epithelial cells is increased, and the level of SP-C and SP-D in BALF was increased ifrst and then decreased.