CAJ | 학술논문

鸡新城疫病毒（Newcastle Disease Virus，NDV）是危害家禽生产的重要病毒之一。试验选取黄芪、紫胡、金银花、野菊花、黄芩、藿香、芦根、细辛、栀子、甘草、马鞭草、鱼腥草、大青叶13种中药的提取液在传代细胞系鸡胚成纤维细胞（DF-1）上进行抗 NDV 试验，通过细胞病变效应（CPE）和噻唑蓝活细胞染色法（MTT），检测13种中药提取液分别对正常 DF-1的细胞毒性以及 NDV 感染 DF-1细胞的预防、治疗和直接灭活作用。结果表明：黄芪、野菊花、马鞭草和细辛提取液对 NDV 有显著地直接灭活作用；黄芪、金银花、野菊花和细辛提取液有显著的阻断 NDV吸附作用；黄芪、金银花、野菊花和马鞭草提取液具有显著抑制 NDV 生物合成作用；综合以上结果说明黄芪、金银花、野菊花、马鞭草和细辛提取液的抗 NDV 作用较好。为抗 NDV 中药研究和临床用药提供了相关的试验数据。
계신성역병독（Newcastle Disease Virus，NDV）시위해가금생산적중요병독지일。시험선취황기、자호、금은화、야국화、황금、곽향、호근、세신、치자、감초、마편초、어성초、대청협13충중약적제취액재전대세포계계배성섬유세포（DF-1）상진행항 NDV 시험，통과세포병변효응（CPE）화새서람활세포염색법（MTT），검측13충중약제취액분별대정상 DF-1적세포독성이급 NDV 감염 DF-1세포적예방、치료화직접멸활작용。결과표명：황기、야국화、마편초화세신제취액대 NDV 유현저지직접멸활작용；황기、금은화、야국화화세신제취액유현저적조단 NDV흡부작용；황기、금은화、야국화화마편초제취액구유현저억제 NDV 생물합성작용；종합이상결과설명황기、금은화、야국화、마편초화세신제취액적항 NDV 작용교호。위항 NDV 중약연구화림상용약제공료상관적시험수거。
To study the inhibiting effects of traditional Chinese medicines (TCM)against the Newcastle Disease Virus (NDV)in DF-1 cell lines,the study using tissue cell culture method and MTT assay to in-vestigate thirteen TCM on the cytotoxicity,antiviral effects by different subtype of prophylaxis,therapy and direct action.The result showed that water extract from Mongolian Milkvetch Root,Wild Dendranthe-ma Flower,European Verbena Herb,Manchurian Wild ginger Herb killed virus.The water extract of Mon-golian Milkvetch Root,Japanese Honeysuckle Flower Bud,Wild Den-dranthema Flower,Manchurian Wild ginger Herb were preventive virus adsorbing cell.The water extract of Mongolian Milkvetch Root,Japa-nese Honeysuckle Flower Bud,Wild Dendranthema Flower,European Verbena Herb inhibited biosynthesis of virus.The study suggests that the Mongolian Milkvetch Root,Japanese Honeysuckle Flower Bud,Wild Dendranthema Flower,European Verbena Herb,Manch-urian Wild ginger Herb have the effect of anti-NDV in vitro.