CAJ | 학술논문

试验采用 HE 常规染色方法，光镜下观察小肠各段肠黏膜上皮内淋巴细胞的分布位置、密度和形态，并对其进行计数，来探索双峰驼肠黏膜免疫机制．结果表明：上皮内淋巴细胞在小肠各段数量分布有显著差异（P ＜0．05），从十二指肠到回肠数量逐渐增多，每5000μm2大小的肠黏膜上皮细胞中，肠上皮内淋巴细胞的平均数量分别为5．40、17．36和33．64个．上皮内淋巴细胞多数位于上皮吸收细胞的基底部，少数分布在上皮细胞核区和核上方，且后者多出现在空肠后段到回肠后段．上皮内淋巴细胞形态单一，多为圆形和椭圆形，与周围上皮吸收细胞的界限非常清晰，有一明显的“空晕”．肠上皮内淋巴细胞从十二指肠到回肠数量逐渐增多，说明了上皮内淋巴细胞的归巢可能与小肠各段的抗原因素差异以及上皮吸收细胞基底侧的 E-钙粘连蛋白表达密切相关．这一结果与在其他动物上的研究结果是不相同的．
시험채용 HE 상규염색방법，광경하관찰소장각단장점막상피내림파세포적분포위치、밀도화형태，병대기진행계수，래탐색쌍봉타장점막면역궤제．결과표명：상피내림파세포재소장각단수량분포유현저차이（P ＜0．05），종십이지장도회장수량축점증다，매5000μm2대소적장점막상피세포중，장상피내림파세포적평균수량분별위5．40、17．36화33．64개．상피내림파세포다수위우상피흡수세포적기저부，소수분포재상피세포핵구화핵상방，차후자다출현재공장후단도회장후단．상피내림파세포형태단일，다위원형화타원형，여주위상피흡수세포적계한비상청석，유일명현적“공훈”．장상피내림파세포종십이지장도회장수량축점증다，설명료상피내림파세포적귀소가능여소장각단적항원인소차이이급상피흡수세포기저측적 E-개점련단백표체밀절상관．저일결과여재기타동물상적연구결과시불상동적．
Using the HE regular dyeing method,the distribution location,density and morphology of the intraepithelial lymphocytes in small intestine were observed,and its number was counted under micro-scope.The results showed that the distribution characteristics of the intraepithelial lymphocytes among the different places of the small intestine were significantly different (P <0.05).The quantity of the intraepi-thelial lymphocytes increased gradually from duodenum to ileum.Within each 5 000 μm2 intestinal mucosal epithelial cells,the average number of intestinal intraepithelial lymphocytes was 5.40,17.36 and 33.64 re-spectively.Most of the intraepithelial lymphocytes were distributed in the base of absorption cell,and a small number of the intraepithelial lymphocytes were distributed near the free surface of epithelial cells. The latter was mainly distributed from the distal jejunum to distal ileum.The cell morphology was round and oval,it's boundary with the surrounding epithelial absorption cell was clear and had a ‘halo’.The study showed that the quantity of intestinal intraepithelial lymphocytes from duodenμm to ileum increased gradu-ally,and the phenomenon further illustrated the homing of intraepithelial lymphocyte was closely related to the E-calcium adhesion proteins expressing on the epithelial basal side of absorptive cells and the different antigens among the different places of the small intestine.