CAJ | 학술논문

为了探究中药复方对雏鸡免疫功能及免疫器官发育的影响．试验以黄芪、白术、防风、黄芩和五倍子等单味中药组成3种中药复方制剂，将120只1日龄健康雏鸡分为4组（Ⅰ组，“扶正止泄散”，未加玉屏风散基础复方组；Ⅱ组，“扶正止泄散”复方组；Ⅲ组，“芪术扶正汤”复方组；Ⅳ组，对照，不加喂药），分别于42、52和62日龄对试验组和对照组鸡进行检测，测定 T 淋巴细胞、鸡γ-干扰素、抗体效价和免疫器官指数等免疫指标，同时制作组织切片、HE 染色，观察其对雏鸡内脏器官发育的影响．结果表明：试验组 T 淋巴细胞 ANAE 阳性率、抗体效价和γ-IFN 水平显著升高（P＜0．01），Ⅰ、Ⅱ组优于Ⅲ组；免疫器官指数和血细胞数量显著高于对照组（P ＜0．01或 P ＜0．05），Ⅱ组优于Ⅰ组；镜下观察组织切片，各器官结构良好无明显病变．3种中药复方对雏鸡免疫功能具有正向促进作用，增强机体细胞及体液免疫功能，其中Ⅱ组具有明显优势．
위료탐구중약복방대추계면역공능급면역기관발육적영향．시험이황기、백술、방풍、황금화오배자등단미중약조성3충중약복방제제，장120지1일령건강추계분위4조（Ⅰ조，“부정지설산”，미가옥병풍산기출복방조；Ⅱ조，“부정지설산”복방조；Ⅲ조，“기술부정탕”복방조；Ⅳ조，대조，불가위약），분별우42、52화62일령대시험조화대조조계진행검측，측정 T 림파세포、계γ-간우소、항체효개화면역기관지수등면역지표，동시제작조직절편、HE 염색，관찰기대추계내장기관발육적영향．결과표명：시험조 T 림파세포 ANAE 양성솔、항체효개화γ-IFN 수평현저승고（P＜0．01），Ⅰ、Ⅱ조우우Ⅲ조；면역기관지수화혈세포수량현저고우대조조（P ＜0．01혹 P ＜0．05），Ⅱ조우우Ⅰ조；경하관찰조직절편，각기관결구량호무명현병변．3충중약복방대추계면역공능구유정향촉진작용，증강궤체세포급체액면역공능，기중Ⅱ조구유명현우세．
This experiment was carried out to explore the effects of traditional Chinese medicines on the immune function and immune organ development of chicken.Three Chinese herbal medicine compound preparations were made by using Astragalus membranaceus ,Atractylodes macrocephala ,Saposhnikovia divaricata ,Scutellaria baicalensis and gallnut.Meanwhile,120 one-day-old chicks were divided into 4 groups,and detected at the ages of 42,52 and 62 days.The detected immune parameters were T lympho-cytes,chickenγ-interferon,antibody titers and immune organ indexes,and the tissue sections and HE stai-ning were also done.The results showed that the T lymphocytes ANAE positive rate,antibody titers andγ-IFN levels of the experimental groups were significantly higher than those of control group(P <0.01),and those of groupsⅠand Ⅱwere higher than those of group Ⅲ.The immune organ indexes and the blood cells number of experimental groups were significantly higher than those of control group(P <0.01 or P <0.05),and those of groupⅡ were higher than those of groupⅠ.The observation results of tissue sections showed that each organ structure without obvious lesions.The experiment indicated that three Chinese herbal medicine compound preparations had positive effects on immune function of chicken,and could im-prove the immune function of body's cell and humor,and the effects of groupⅡ was the most obviously.