中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
33期
5312-5317
,共6页
李红专%刘行海%牛彩琴%张团笑
李紅專%劉行海%牛綵琴%張糰笑
리홍전%류행해%우채금%장단소
组织构建%组织工程%附子%血管%内皮细胞%血管舒张%一氧化氮%血管张力%离体
組織構建%組織工程%附子%血管%內皮細胞%血管舒張%一氧化氮%血管張力%離體
조직구건%조직공정%부자%혈관%내피세포%혈관서장%일양화담%혈관장력%리체
Aconitum%Aorta%Endothelial Cells%Vasodilatio
背景：附子为毛莨科乌头子根的加工品，具有温肾回阳，回阳救逆和温里散寒之功效。研究发现其具有明显的强心、降压、扩张血管、镇痛、抗炎及毒性等作用。目的：观察附子水煎剂对主动脉环的舒张作用及机制。方法：采用家兔离体主动脉平滑肌标本，以去甲肾上腺素(10-6 mol/L)预收缩主动脉后给予0.5，1.0，2.0，4.0，8.0 g/L附子水煎剂观察其张力变化。然后将不同浓度的附子水煎剂干预的家兔离体主动脉平滑肌加入去除血管内皮、给予一氧化氮合酶抑制剂左旋硝基精氨酸(1×10-4mol/L)、甲烯蓝(1×10-5 mol/L)、环氧酶抑制剂吲哚美辛(1×10-5 mol/L)和β肾上腺素能受体阻断剂普萘洛尔(1×10-5 mol/L)，探讨它们对血管张力变化的影响。结果与结论：附子水煎剂对血管环静息张力无明显影响，但不同剂量的附子水煎剂可使10-6 mol/L去甲肾上腺素预收缩血管产生明显舒张。去除血管内皮、给予左旋硝基精氨酸或给予甲烯蓝可减弱附子水煎剂对血管的舒张作用，但吲哚美辛和普萘洛尔对此无影响。另外，4 g/L附子水煎剂对无内皮细胞血管环去甲肾上腺素和KCl的量效收缩反应无明显影响。提示附子对主动脉的舒张作用是内皮依赖性，与内皮细胞释放的一氧化氮有关，而与平滑肌细胞膜上的受体依赖性Ca2+通道和电压依赖性Ca2+无关。
揹景：附子為毛莨科烏頭子根的加工品，具有溫腎迴暘，迴暘救逆和溫裏散寒之功效。研究髮現其具有明顯的彊心、降壓、擴張血管、鎮痛、抗炎及毒性等作用。目的：觀察附子水煎劑對主動脈環的舒張作用及機製。方法：採用傢兔離體主動脈平滑肌標本，以去甲腎上腺素(10-6 mol/L)預收縮主動脈後給予0.5，1.0，2.0，4.0，8.0 g/L附子水煎劑觀察其張力變化。然後將不同濃度的附子水煎劑榦預的傢兔離體主動脈平滑肌加入去除血管內皮、給予一氧化氮閤酶抑製劑左鏇硝基精氨痠(1×10-4mol/L)、甲烯藍(1×10-5 mol/L)、環氧酶抑製劑吲哚美辛(1×10-5 mol/L)和β腎上腺素能受體阻斷劑普萘洛爾(1×10-5 mol/L)，探討它們對血管張力變化的影響。結果與結論：附子水煎劑對血管環靜息張力無明顯影響，但不同劑量的附子水煎劑可使10-6 mol/L去甲腎上腺素預收縮血管產生明顯舒張。去除血管內皮、給予左鏇硝基精氨痠或給予甲烯藍可減弱附子水煎劑對血管的舒張作用，但吲哚美辛和普萘洛爾對此無影響。另外，4 g/L附子水煎劑對無內皮細胞血管環去甲腎上腺素和KCl的量效收縮反應無明顯影響。提示附子對主動脈的舒張作用是內皮依賴性，與內皮細胞釋放的一氧化氮有關，而與平滑肌細胞膜上的受體依賴性Ca2+通道和電壓依賴性Ca2+無關。
배경：부자위모랑과오두자근적가공품，구유온신회양，회양구역화온리산한지공효。연구발현기구유명현적강심、강압、확장혈관、진통、항염급독성등작용。목적：관찰부자수전제대주동맥배적서장작용급궤제。방법：채용가토리체주동맥평활기표본，이거갑신상선소(10-6 mol/L)예수축주동맥후급여0.5，1.0，2.0，4.0，8.0 g/L부자수전제관찰기장력변화。연후장불동농도적부자수전제간예적가토리체주동맥평활기가입거제혈관내피、급여일양화담합매억제제좌선초기정안산(1×10-4mol/L)、갑희람(1×10-5 mol/L)、배양매억제제신타미신(1×10-5 mol/L)화β신상선소능수체조단제보내락이(1×10-5 mol/L)，탐토타문대혈관장력변화적영향。결과여결론：부자수전제대혈관배정식장력무명현영향，단불동제량적부자수전제가사10-6 mol/L거갑신상선소예수축혈관산생명현서장。거제혈관내피、급여좌선초기정안산혹급여갑희람가감약부자수전제대혈관적서장작용，단신타미신화보내락이대차무영향。령외，4 g/L부자수전제대무내피세포혈관배거갑신상선소화KCl적량효수축반응무명현영향。제시부자대주동맥적서장작용시내피의뢰성，여내피세포석방적일양화담유관，이여평활기세포막상적수체의뢰성Ca2+통도화전압의뢰성Ca2+무관。
BACKGROUND:Aconitum carmichaeli Debx (ACD) is the tuberous root of Aconium carmicgaekum, used as cardiotonic to restore yang for the treatment of colapse and shock, to warm the kidney and reinforceyang, and to expel cold and promote the flow ofyang-qi. Studies have found that ACD has obviously cardiotonic, antihypertensive, vasodilatory, analgesic, anti-inflammatory and toxic effects. OBJESTIVE: To observe the vasodilatory effects of a water decoction of ACD on rabbit’s aorta rings and its mechanism. METHODS:Rabbits aorta arteries were isolated, pre-contracted with noradrenaline (10-6 mol/L) and their responses to different concentrations of ACD (0.5, 1.0, 2.0, 4.0, 8.0 g/L) were investigated. The effects of removal of vascular endothelium and different signaling pathway inhibitors (Nω-nitro-L-arginine: 1×10-4 mol/L, methylene blue: 1×10-5 mol/L, indomethacin: 1×10-5 mol/L, propranolol: 1×10-5 mol/L) on ACD-induced vasodilation were also assessed. RESULTS AND CONCLUSION:ACD could not change the resting tension of rabbit aortic rings, but ACD treatment resulted in an obvious relaxation in narodrenaline-precontracted aortic rings and the relaxant effect was dose-dependent. The vasodilatory effect of ACD was significantly reduced by removal of endothelium, 1×10-4 mol/L Nω-nitro-L-arginine and 1×10-5 mol/L methylene blue but not reduced by indomethacin and propranolol. In addition, 4 g/L water decoction of ACD did not decrease the dose-response curves of artery rings to narodrenaline or KCl in the absence of endothelial cels. ACD can relax isolated rabbit’s aorta, which may be related to endothelium-released nitric oxide, but has no significant relevance with receptor-operated and voltage-dependent calcium channels.
