临床儿科杂志
臨床兒科雜誌
림상인과잡지
Journal of Clinical Pediatrics
2015年
9期
788-791
,共4页
哮喘%SLC26A9基因%易感性%儿童
哮喘%SLC26A9基因%易感性%兒童
효천%SLC26A9기인%역감성%인동
asthma%SLC26A9 gene%susceptibility%child
目的：探讨我国中部地区汉族儿童中SLC26A9基因SNP位点（rs2282430和rs12031234）多态性与哮喘及其临床特点的关系。方法利用病例对照的研究方法，选取203例哮喘患儿和221例健康儿童为研究对象，利用限制性片段长度多态性分析（PCR-RFLP）的方法检测两个SNP位点多态性，并进行统计分析。结果哮喘患儿与对照组之间，SNP位点rs2282430的3种基因型（AA、AG、GG）分布差异有统计学意义（P=0.042），哮喘患儿AA基因型的比例较高；在隐性模式下（AA对AG+GG），两组相比差异有统计学意义（P=0.028）。哮喘患儿SNP位点rs2282430的A等位基因频率高于对照儿童，差异有统计学意义（P=0.011），提示A等位基因为风险因子。而哮喘患儿与对照组儿童之间，SNP位点rs12031234的3种基因型（TT、GT、GG）分布差异无统计学意义（P=0.479），等位基因频率差异也无统计学意义（P=0.215）。哮喘患儿SNP位点rs2282430不同基因型之间，淋巴细胞总数、中性粒细胞百分比、C反应蛋白、免疫球蛋白E、以及嗜酸性粒细胞百分比的差异均无统计学意义（P>0.05）。结论 SLC26A9基因SNP位点rs2282430的多态性与我国中部地区儿童哮喘的易感性相关；但该位点的多态性与LYM、CRP、IgE、NEU%以及EOS%等指标不具有相关性。
目的：探討我國中部地區漢族兒童中SLC26A9基因SNP位點（rs2282430和rs12031234）多態性與哮喘及其臨床特點的關繫。方法利用病例對照的研究方法，選取203例哮喘患兒和221例健康兒童為研究對象，利用限製性片段長度多態性分析（PCR-RFLP）的方法檢測兩箇SNP位點多態性，併進行統計分析。結果哮喘患兒與對照組之間，SNP位點rs2282430的3種基因型（AA、AG、GG）分佈差異有統計學意義（P=0.042），哮喘患兒AA基因型的比例較高；在隱性模式下（AA對AG+GG），兩組相比差異有統計學意義（P=0.028）。哮喘患兒SNP位點rs2282430的A等位基因頻率高于對照兒童，差異有統計學意義（P=0.011），提示A等位基因為風險因子。而哮喘患兒與對照組兒童之間，SNP位點rs12031234的3種基因型（TT、GT、GG）分佈差異無統計學意義（P=0.479），等位基因頻率差異也無統計學意義（P=0.215）。哮喘患兒SNP位點rs2282430不同基因型之間，淋巴細胞總數、中性粒細胞百分比、C反應蛋白、免疫毬蛋白E、以及嗜痠性粒細胞百分比的差異均無統計學意義（P>0.05）。結論 SLC26A9基因SNP位點rs2282430的多態性與我國中部地區兒童哮喘的易感性相關；但該位點的多態性與LYM、CRP、IgE、NEU%以及EOS%等指標不具有相關性。
목적：탐토아국중부지구한족인동중SLC26A9기인SNP위점（rs2282430화rs12031234）다태성여효천급기림상특점적관계。방법이용병례대조적연구방법，선취203례효천환인화221례건강인동위연구대상，이용한제성편단장도다태성분석（PCR-RFLP）적방법검측량개SNP위점다태성，병진행통계분석。결과효천환인여대조조지간，SNP위점rs2282430적3충기인형（AA、AG、GG）분포차이유통계학의의（P=0.042），효천환인AA기인형적비례교고；재은성모식하（AA대AG+GG），량조상비차이유통계학의의（P=0.028）。효천환인SNP위점rs2282430적A등위기인빈솔고우대조인동，차이유통계학의의（P=0.011），제시A등위기인위풍험인자。이효천환인여대조조인동지간，SNP위점rs12031234적3충기인형（TT、GT、GG）분포차이무통계학의의（P=0.479），등위기인빈솔차이야무통계학의의（P=0.215）。효천환인SNP위점rs2282430불동기인형지간，림파세포총수、중성립세포백분비、C반응단백、면역구단백E、이급기산성립세포백분비적차이균무통계학의의（P>0.05）。결론 SLC26A9기인SNP위점rs2282430적다태성여아국중부지구인동효천적역감성상관；단해위점적다태성여LYM、CRP、IgE、NEU%이급EOS%등지표불구유상관성。
ObjectiveTo investigate the association between nucleotide polymorphisms (SNP) of rs2282430 and rs2031234 inSLC26A9 gene and clinical characteristics of asthma in Han children in central China.MethodsA case-control study was performed. Two hundreds and three children with asthma were recruited in this study and 221 normal children were selected as controls. The genotypes of two SNPs inSLC26A9 gene were examined using PCR-RFLP.ResultsBetween children with asthma and controls, the distribution of three genotypes (AA, AG and GG) in rs2282430 locus had signiifcant difference (P=0.042). The percentage of AA genotype was higher in children with asthma than that in controls. In implicit mode (AAvs. AG+GG), the two groups was statistically signiifcant difference (P=0.028). The frequency of A allele was higher in children with asthma than that in controls (P=0.011). Between children with asthma and controls, the distribution of three genotypes (TT, GT, and GG) in rs12031234 locus had no signiifcant difference (P=0.479). The frequency of alleles in rs12031234 locus also had no signiifcant difference (P=0.215). Among asthmatic children with different genotype of rs2282430, the lymphocytecounts (LYM), C-reaction protein (CRP), IgE, neutrophils (NEU%), and eosinophils (EOS%) were not signiifcantly different (P>0.05). Conclu-sionsThe rs2282430 polymorphism inSLC26A9 gene is associated with childhood asthma in the central China and the A allele is the risk factor. The rs2282430 polymorphism is not associated with LYM counts, CRP level, IgE level, NEU%, and EOS%.
