CAJ | 학술논문

背景：缺氧诱导因子1α在缺氧的环境下对细胞凋亡起着双重调控作用。缺氧的严重程度决定细胞是出现凋亡还是适应缺氧生存。当细胞暴露于慢性或极度缺氧时由缺氧诱导因子1α引起的保护机制不足而发生凋亡。目的：观察缺氧诱导因子1α和凋亡在人不同突出类型腰椎髓核组织中的表达，判断两者有无相关性。方法：腰椎髓核组织标本取自腰椎间盘突出症行腰椎后路椎板开窗髓核摘除患者60例，41例取自L4-5髓核组织，19例取自 L5-S1髓核组织。将髓核组织分为突出组、游离组各30例，同时选取腰椎骨折脱位患者腰椎髓核组织标本10例作为对照组。采用免疫组织化学技术，观察各组突出腰椎髓核组织缺氧诱导因子1α的表达；采用Tunel技术，观察不同突出类型腰椎髓核细胞凋亡程度；分析各组髓核组织缺氧诱导因子1α的表达与髓核细胞凋亡率有无相关性。结果与结论：游离组、突出组和对照组都可观察到缺氧诱导因子1α的表达，游离组缺氧诱导因子1α在髓核的表达显著高于突出组和对照组(P<0.01)。3组都可观察到髓核细胞的凋亡，游离组髓核细胞凋亡的表达显著高于突出型和对照组(P <0.01)。髓核细胞缺氧诱导因子1α表达和凋亡呈正相关(P <0.01)。结果提示，腰椎间盘突出症髓核组织中缺氧诱导因子1α的表达与突出类型有关，在脱垂游离型中表达最高。腰椎髓核组织中缺氧诱导因子1α的表达与凋亡率呈正相关。
배경：결양유도인자1α재결양적배경하대세포조망기착쌍중조공작용。결양적엄중정도결정세포시출현조망환시괄응결양생존。당세포폭로우만성혹겁도결양시유결양유도인자1α인기적보호궤제불족이발생조망。목적：관찰결양유도인자1α화조망재인불동돌출류형요추수핵조직중적표체，판단량자유무상관성。방법：요추수핵조직표본취자요추간반돌출증행요추후로추판개창수핵적제환자60례，41례취자L4-5수핵조직，19례취자 L5-S1수핵조직。장수핵조직분위돌출조、유리조각30례，동시선취요추골절탈위환자요추수핵조직표본10례작위대조조。채용면역조직화학기술，관찰각조돌출요추수핵조직결양유도인자1α적표체；채용Tunel기술，관찰불동돌출류형요추수핵세포조망정도；분석각조수핵조직결양유도인자1α적표체여수핵세포조망솔유무상관성。결과여결론：유리조、돌출조화대조조도가관찰도결양유도인자1α적표체，유리조결양유도인자1α재수핵적표체현저고우돌출조화대조조(P<0.01)。3조도가관찰도수핵세포적조망，유리조수핵세포조망적표체현저고우돌출형화대조조(P <0.01)。수핵세포결양유도인자1α표체화조망정정상관(P <0.01)。결과제시，요추간반돌출증수핵조직중결양유도인자1α적표체여돌출류형유관，재탈수유리형중표체최고。요추수핵조직중결양유도인자1α적표체여조망솔정정상관。
BACKGROUND:Under hypoxic environment, hypoxia-inducible factor 1α plays a dualregulatory role in cel apoptosis. Severity of hypoxia is the key to determine whether cels appear to have apoptosis or adapt to survive. When the cels are exposed to chronic or extreme hypoxia, a lack of protection mechanisms from hypoxia-inducible factor-1α can induce cel apoptosis. OBJECTIVE: To research the expression of hypoxia-inducible factor 1α in human lumbar nucleus pulposus of different herniated types and its relationships with cel apoptosis. METHODS: The nucleus pulposus was harvested from 60 cases of herniation of lumbar intervertebral discs, L4-5 in 41 cases and L5-S1 in 19 cases. The nucleus pulposus tissues were equaly divided into protruded and sequestered groups. Meanwhile, the nucleus pulposus tissues from another 10 cases of lumbar spine fracture were taken as control group. Expression of hypoxia-inducible factor 1α and apoptosis of lumbar nucleus pulposus cels were observed and detected with immunohistochemical technique and TUNEL method. Correlation of hypoxia-inducible factor 1α and apoptosis in human lumbar nucleus pulposus of different herniated types was analyzed. RESULTS AND CONCLUSION: The expression of hypoxia-inducible factor 1α was visualized in each case, but it was significantly higher in the sequestered group than in the protruded group and control group (P < 0.01). Apoptosis of nucleus pulposus cels were found in al the three groups, but the apoptotic rate was also higher in the sequestered group than in the protruded group and control group (P < 0.01). Expression of hypoxia-inducible factor 1α was positively correlated with cel apoptosis in human lumbar nucleus pulposus (P < 0.01). Overal, the expression of hypoxia-inducible factor1α in degenerative human lumbar nucleus pulposus is associated with herniated types, which is the highest in the sequestered type. The relationship between hypoxia-inducible factor 1α and apoptosis is positive.