CAJ | 학술논문

目的：观察氮乙酰半胱氨酸（ NAC）对心肌肥厚的作用并探讨其与炎性因子表达的关系。方法 SD大鼠随机分为4组，假手术组、模型组、假手术药物组及模型药物组，腹主动脉缩窄法建立压力负荷模型。药物组灌胃 NAC （0．2 g? kg－1? d－1）干预8周。通过组织病理和心脏超声评估心肌的肥厚程度，比色法检测胶原含量，RT－PCR和ELISA法测定炎性细胞因子表达水平，用Fenton原理测定心肌内活性氧（ ROS ）水平。结果与假手术组比较，模型组大鼠的血压升高，且左室重量指数、心肌细胞内径、胶原含量及左室室壁厚度均明显升高（P＜0．05）；心肌内的肿瘤坏死因子（TNF－α）、白细胞介素－6（IL－6） mRNA和蛋白水平也显著升高（ P＜0．05）。 NAC干预后，心肌组织的ROS水平明显下降，左室重量指数、胶原含量以及左室室壁厚度均较模型组明显降低（ P＜0．05）。模型药物组的TNF－α、IL－6 mRNA和蛋白水平均较模型组显著下降（P＜0．05）；但IL－10蛋白含量与假手术组比较差异不显著。结论 NAC可改善压力负荷大鼠心肌肥厚、抑制促炎性细胞因子表达可能是其重要分子机制之一。
목적：관찰담을선반광안산（ NAC）대심기비후적작용병탐토기여염성인자표체적관계。방법 SD대서수궤분위4조，가수술조、모형조、가수술약물조급모형약물조，복주동맥축착법건립압력부하모형。약물조관위 NAC （0．2 g? kg－1? d－1）간예8주。통과조직병리화심장초성평고심기적비후정도，비색법검측효원함량，RT－PCR화ELISA법측정염성세포인자표체수평，용Fenton원리측정심기내활성양（ ROS ）수평。결과여가수술조비교，모형조대서적혈압승고，차좌실중량지수、심기세포내경、효원함량급좌실실벽후도균명현승고（P＜0．05）；심기내적종류배사인자（TNF－α）、백세포개소－6（IL－6） mRNA화단백수평야현저승고（ P＜0．05）。 NAC간예후，심기조직적ROS수평명현하강，좌실중량지수、효원함량이급좌실실벽후도균교모형조명현강저（ P＜0．05）。모형약물조적TNF－α、IL－6 mRNA화단백수평균교모형조현저하강（P＜0．05）；단IL－10단백함량여가수술조비교차이불현저。결론 NAC가개선압력부하대서심기비후、억제촉염성세포인자표체가능시기중요분자궤제지일。
Objective To investigate the effects of N -acetylcystein ( NAC) on the cardiac hypertrophy in pressure -overload rats and the relationship with the expression of inflammatory cytokines.Methods The SD rats were randomly divided into four groups:sham group, model group , drug group in sham and drug group in model.Pressure-overload rat model was established by abdominal aortic coarctation.Rats in drug group were treated with NAC ( 0.2 g? kg-1? d -1 , ig ) for 8 weeks. Cardiac hypertrophy was evaluated by heart histological analysis and echocardiography.The collagen content was determined by chromatome-try.RT-PCR and ELISA were used to determine the expression of in-flammatory cytokines.The production of reactive oxygen species ( ROS) were measured by Fenton reaction.Results Compared with sham rats, blood pressure, left ventricular weight index( LVWI) , myocyte diameter, left ventricular wall thick(LVWT) as well as the collagen content( CC) in model rats significantly elevated ( P <0.05 ) .In model rats treated with NAC, LVWI, LVWT and CC were significantly decreased as well as the production of ROS ( P <0.05 ) . Furthermore, the expression of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)but IL-10 was elevated in myocardium of pressure-o-verload rats, which was inhibited by NAC( P<0.05).NAC had no effects on the expression of IL-10.Conclusion NAC could improve the cardiac hypertrophy induced by pressure-overload.The effects of NAC probably relates to the decrease of pro-inflammatory cytokines expression in rat myocardium.