南昌大学学报（医学版）
南昌大學學報（醫學版）
남창대학학보（의학판）
Acta Academiae Medicinae Jiangxi
2015年
4期
60-64,85
,共6页
干眼症%干燥综合征%趋化因子受体表型 5%免疫组织化学%流式细胞术
榦眼癥%榦燥綜閤徵%趨化因子受體錶型 5%免疫組織化學%流式細胞術
간안증%간조종합정%추화인자수체표형 5%면역조직화학%류식세포술
dry eye disease%sicca syndrome group%chemokine receptor 5%immunohistochemistry%flow cytometry
目的：探讨泪膜和眼表趋化因子受体5（CCR5）及其配体在干眼症中的表达和临床意义。方法选择干眼症患者44例，其中干燥综合征患者（干燥综合征组）17例，非干燥综合征患者（非干燥综合征组）27例。以健康体检者20例作为对照组。对3组分别采用酶联免疫吸附法检测 CCL3、CCL4和 CCL5表达水平，免疫组织化学法检测结膜上皮 CCL3、CCL4、CCL5表达水平和 CCR5＋细胞密度，流式细胞术检测结膜上皮 CCR4＋CD4＋、CCR5＋CD4＋和 CCR6＋CD4＋细胞比例。对干燥综合征组 CCL3、CCL4、CCL5表达水平与检测指标的相关性进行分析。结果干燥综合征组结膜上皮 CCL3、CCL4和 CCL5表达水平均显著高于非干燥综合征组，非干燥综合征组、干燥综合征组结膜上皮 CCL3、CCL4和 CCL5表达水平则均显著高于对照组（均 P ＜0．01）。干燥综合征组 CCR5＋CD4＋、CCR6＋CD4＋细胞比例明显高于非干燥综合征组，非干燥综合征组、干燥综合征组 CCR5＋CD4＋、CCR6＋CD4＋细胞比例则均显著高于对照组（P ＜0．05或 P ＜0．01）。干燥综合征组 CCL3、CCL4、CCL5表达水平与泪液清除率、结膜杯状细胞密度均呈正相关（r ＝0．446、0．693、0．656、0．712、0．768和0．780，均 P ＜0．05）。结论CCR5及其配体在干眼症泪膜和眼表中的表达均显著增高，CCL5表达可能与干眼症严重程度有关。
目的：探討淚膜和眼錶趨化因子受體5（CCR5）及其配體在榦眼癥中的錶達和臨床意義。方法選擇榦眼癥患者44例，其中榦燥綜閤徵患者（榦燥綜閤徵組）17例，非榦燥綜閤徵患者（非榦燥綜閤徵組）27例。以健康體檢者20例作為對照組。對3組分彆採用酶聯免疫吸附法檢測 CCL3、CCL4和 CCL5錶達水平，免疫組織化學法檢測結膜上皮 CCL3、CCL4、CCL5錶達水平和 CCR5＋細胞密度，流式細胞術檢測結膜上皮 CCR4＋CD4＋、CCR5＋CD4＋和 CCR6＋CD4＋細胞比例。對榦燥綜閤徵組 CCL3、CCL4、CCL5錶達水平與檢測指標的相關性進行分析。結果榦燥綜閤徵組結膜上皮 CCL3、CCL4和 CCL5錶達水平均顯著高于非榦燥綜閤徵組，非榦燥綜閤徵組、榦燥綜閤徵組結膜上皮 CCL3、CCL4和 CCL5錶達水平則均顯著高于對照組（均 P ＜0．01）。榦燥綜閤徵組 CCR5＋CD4＋、CCR6＋CD4＋細胞比例明顯高于非榦燥綜閤徵組，非榦燥綜閤徵組、榦燥綜閤徵組 CCR5＋CD4＋、CCR6＋CD4＋細胞比例則均顯著高于對照組（P ＜0．05或 P ＜0．01）。榦燥綜閤徵組 CCL3、CCL4、CCL5錶達水平與淚液清除率、結膜杯狀細胞密度均呈正相關（r ＝0．446、0．693、0．656、0．712、0．768和0．780，均 P ＜0．05）。結論CCR5及其配體在榦眼癥淚膜和眼錶中的錶達均顯著增高，CCL5錶達可能與榦眼癥嚴重程度有關。
목적：탐토루막화안표추화인자수체5（CCR5）급기배체재간안증중적표체화림상의의。방법선택간안증환자44례，기중간조종합정환자（간조종합정조）17례，비간조종합정환자（비간조종합정조）27례。이건강체검자20례작위대조조。대3조분별채용매련면역흡부법검측 CCL3、CCL4화 CCL5표체수평，면역조직화학법검측결막상피 CCL3、CCL4、CCL5표체수평화 CCR5＋세포밀도，류식세포술검측결막상피 CCR4＋CD4＋、CCR5＋CD4＋화 CCR6＋CD4＋세포비례。대간조종합정조 CCL3、CCL4、CCL5표체수평여검측지표적상관성진행분석。결과간조종합정조결막상피 CCL3、CCL4화 CCL5표체수평균현저고우비간조종합정조，비간조종합정조、간조종합정조결막상피 CCL3、CCL4화 CCL5표체수평칙균현저고우대조조（균 P ＜0．01）。간조종합정조 CCR5＋CD4＋、CCR6＋CD4＋세포비례명현고우비간조종합정조，비간조종합정조、간조종합정조 CCR5＋CD4＋、CCR6＋CD4＋세포비례칙균현저고우대조조（P ＜0．05혹 P ＜0．01）。간조종합정조 CCL3、CCL4、CCL5표체수평여루액청제솔、결막배상세포밀도균정정상관（r ＝0．446、0．693、0．656、0．712、0．768화0．780，균 P ＜0．05）。결론CCR5급기배체재간안증루막화안표중적표체균현저증고，CCL5표체가능여간안증엄중정도유관。
ABSTRACT:Objective To investigate the expression and significance of chemokine receptor 5 (CCR5)and its ligands in tear film and ocular surface of patients with dry eye disease.Methods Forty-four patients with dry eye disease were divided into two groups:sicca syndrome group(17 cases)and non-sicca syndrome group(27 cases).In addition,20 healthy subjects were selected as the control group.The concentrations of CCL3,CCL4 and CCL5 were measured using enzyme-linked immunosorbent assay.The expression of CCL3,CCL4 and CCL5 and the density of CCR5 +cells were determined using immunohistochemistry.The percentages of CCR4 + CD4 + ,CCR5 +CD4+ and CCR6+CD4+ cells in conjunctival epithelium were detected using flow cytometry.Fur-thermore,the correlation between chemokine levels and tear film and ocular surface parameters was analyzed in sicca syndrome group.Results Compared with non-sicca syndrome group,the ex-pression of CCL3,CCL4 and CCL5 and the percentages of CCR5+CD4+ and CCR6+CD4 + cells in conjunctival epithelium significantly increased in sicca syndrome group(P <0.01).Compared with healthy subjects,the expression of CCL3,CCL4 and CCL5 and the percentages of CCR5 +CD4+ and CCR6 +CD4+ cells in conjunctival epithelium significantly increased in patients with dry eye disease(P <0.05 or P <0.01).The expression of CCL3,CCL4 and CCL5 was positively correlated with tear clearance rate(r=0.446,0.693 and 0.656,respectively,P <0.05)and corneal goblet cell density(r=0.712,0.768 and 0.780,respectively,P <0.05).Conclusion The expres-sion of CCR5 and its ligands increases in the tear film and ocular surface of patients with dry eye disease.CCL5 expression is correlated with the severity of dry eye disease.
