CAJ | 학술논문

目的：观察惊恐伤肾对大鼠记忆力的影响及左归丸的脑保护作用。方法将40只雌性Waster大鼠按体重随机分为4组，分别为假手术组、脑缺血模型组、肾虚模型组与实验组，后3组进行大脑中动脉阻塞（ MCAO）造模手术；肾虚模型组于MCAO手术前10天，用“猫吓鼠”法进行恐吓，实验组于MCAO手术前3天，灌胃1．62 g? kg－1左归丸汤剂2 mL，每日1次，连续给药10 d，其他3组给予等体积生理盐水。新奇物认知法检测大鼠记忆力；免疫组化法及免疫印迹法检测脑源性神经营养因子（ BDNF）的表达。结果与假手术组比较，脑缺血模型组大鼠记忆力下降；与脑缺血模型组比较，肾虚模型组大鼠记忆力下降且BDNF表达明显下调（ P＜0．05）。与肾虚模型组比较，实验组大鼠记忆力明显改善，同时BDNF表达量明显增加（ P＜0．05）。结论惊恐伤肾可加重大鼠缺血后记忆障碍，左归丸可以改善惊恐伤肾大鼠缺血后记忆力，这种作用有可能通过上调BDNF来实现。
목적：관찰량공상신대대서기억력적영향급좌귀환적뇌보호작용。방법장40지자성Waster대서안체중수궤분위4조，분별위가수술조、뇌결혈모형조、신허모형조여실험조，후3조진행대뇌중동맥조새（ MCAO）조모수술；신허모형조우MCAO수술전10천，용“묘하서”법진행공하，실험조우MCAO수술전3천，관위1．62 g? kg－1좌귀환탕제2 mL，매일1차，련속급약10 d，기타3조급여등체적생리염수。신기물인지법검측대서기억력；면역조화법급면역인적법검측뇌원성신경영양인자（ BDNF）적표체。결과여가수술조비교，뇌결혈모형조대서기억력하강；여뇌결혈모형조비교，신허모형조대서기억력하강차BDNF표체명현하조（ P＜0．05）。여신허모형조비교，실험조대서기억력명현개선，동시BDNF표체량명현증가（ P＜0．05）。결론량공상신가가중대서결혈후기억장애，좌귀환가이개선량공상신대서결혈후기억력，저충작용유가능통과상조BDNF래실현。
Objective To observe the effects of Zuogui Wan on the memory of the rats with panic injury and the neuroprotective effect . Methods Forty female Waster rats were randomly divided into 4 groups: sham group, model -brain group, model -kidney group, test group.Middle cerebral artery occlusion( MCAO) was operated in rats ex-cept sham group,and ischemia-reperfusion were executed 2 hours after MCAO.The model-kidney group was threatened by the cat in 10 days before MCAO operation.The rats in the test group orally 1.62 g? kg -1 Zuogui Wan Decoction 2 mL at the time of 3 days before MCAO opera-tion , 1 times a day continuously for 10 days.The other 3 groups were giv-en equal volume of saline.The memory in each group rats was detected by novel object recognition task.The neuron injury in rats was observed by HE staining.The expression of brain -derived neurotrophic factor ( BDNF ) was assessed by immunohistochemistry and Western blot.Results Compared with sham group, memory decline after MCAO in model -brain group rats.Compared with the model -brain group, memory impairment was aggravated induced by ischemia and downregu-lated of expression of BDNF in model-kidney group,which was statisti-cally difference ( P<0.05 ) .Compared with the model-kidney group memory in rats were significantly improved and the expression of BDNF was increased in the test group,which was statistically difference (P<0.05).Conclusion Kidney deficiency induced by panic can significant aggravate memory impairment after cerebral ischemic injury.Zuogui Wan can improve memory ability of rats of kidney deficiency induced by panic after ischemia,which is likely achieved by upregulation of the ex-pression of BDNF.