中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
33期
5259-5263
,共5页
组织构建%骨组织工程%骨关节炎%白细胞介素6%基质金属蛋白酶3
組織構建%骨組織工程%骨關節炎%白細胞介素6%基質金屬蛋白酶3
조직구건%골조직공정%골관절염%백세포개소6%기질금속단백매3
Osteoarthritis%Interleukin-6%Matrix Metalloproteinases
背景：已有实验证实膝骨关节炎的发病与炎症细胞因子有密切的关系。目的：研究骨性关节炎患者关节液中白细胞介素6和基质金属蛋白酶3水平及其相关性，以及两者与骨性关节炎严重程度之间的相关性。方法：收集关节穿刺治疗的骨性关节炎患者关节液标本78份，应用ELISA法检测标本中白细胞介素6和基质金属蛋白酶3质量浓度，并对患者骨性关节炎严重程度进行HSS评分，采用Pearson相关分析探讨白细胞介素6、基质金属蛋白酶3和美国纽约特种外科医院(HSS)评分两两之间的相关性。结果与结论：骨性关节炎患者膝关节液中白细胞介素6为(13.1±5.7)μg/L，基质金属蛋白酶3为(989.3±429.5)μg/L， HSS为(56.4±12.0)分。Pearson相关分析显示白细胞介素6、基质金属蛋白酶3的表达水平呈显著性正相关关系(r=0.70，P <0.001)；白细胞介素6和基质金属蛋白酶3均与关节美国纽约特种外科医院(HSS)评分均呈负相关关系(r=-0.70，r=-0.75，P<0.001)。结果显性关节液中白细胞介素6和基质金属蛋白酶3水平之间，以及两者与骨性关节炎严重程度之间均呈正相关，提示测定骨性关节炎患者关节液中白细胞介素6和基质金属蛋白酶3水平对早期诊断和治疗具有重要意义。
揹景：已有實驗證實膝骨關節炎的髮病與炎癥細胞因子有密切的關繫。目的：研究骨性關節炎患者關節液中白細胞介素6和基質金屬蛋白酶3水平及其相關性，以及兩者與骨性關節炎嚴重程度之間的相關性。方法：收集關節穿刺治療的骨性關節炎患者關節液標本78份，應用ELISA法檢測標本中白細胞介素6和基質金屬蛋白酶3質量濃度，併對患者骨性關節炎嚴重程度進行HSS評分，採用Pearson相關分析探討白細胞介素6、基質金屬蛋白酶3和美國紐約特種外科醫院(HSS)評分兩兩之間的相關性。結果與結論：骨性關節炎患者膝關節液中白細胞介素6為(13.1±5.7)μg/L，基質金屬蛋白酶3為(989.3±429.5)μg/L， HSS為(56.4±12.0)分。Pearson相關分析顯示白細胞介素6、基質金屬蛋白酶3的錶達水平呈顯著性正相關關繫(r=0.70，P <0.001)；白細胞介素6和基質金屬蛋白酶3均與關節美國紐約特種外科醫院(HSS)評分均呈負相關關繫(r=-0.70，r=-0.75，P<0.001)。結果顯性關節液中白細胞介素6和基質金屬蛋白酶3水平之間，以及兩者與骨性關節炎嚴重程度之間均呈正相關，提示測定骨性關節炎患者關節液中白細胞介素6和基質金屬蛋白酶3水平對早期診斷和治療具有重要意義。
배경：이유실험증실슬골관절염적발병여염증세포인자유밀절적관계。목적：연구골성관절염환자관절액중백세포개소6화기질금속단백매3수평급기상관성，이급량자여골성관절염엄중정도지간적상관성。방법：수집관절천자치료적골성관절염환자관절액표본78빈，응용ELISA법검측표본중백세포개소6화기질금속단백매3질량농도，병대환자골성관절염엄중정도진행HSS평분，채용Pearson상관분석탐토백세포개소6、기질금속단백매3화미국뉴약특충외과의원(HSS)평분량량지간적상관성。결과여결론：골성관절염환자슬관절액중백세포개소6위(13.1±5.7)μg/L，기질금속단백매3위(989.3±429.5)μg/L， HSS위(56.4±12.0)분。Pearson상관분석현시백세포개소6、기질금속단백매3적표체수평정현저성정상관관계(r=0.70，P <0.001)；백세포개소6화기질금속단백매3균여관절미국뉴약특충외과의원(HSS)평분균정부상관관계(r=-0.70，r=-0.75，P<0.001)。결과현성관절액중백세포개소6화기질금속단백매3수평지간，이급량자여골성관절염엄중정도지간균정정상관，제시측정골성관절염환자관절액중백세포개소6화기질금속단백매3수평대조기진단화치료구유중요의의。
BACKGROUND:It is confirmed that the onset of osteoarthritis is closely related to inflammatory cytokines. OBJECTIVE: To investigate the levels of interleukin-6 and matrix metaloproteinase-3 in the synovial fluid of patients with osteoarthritis and their correlation with the severity of osteoarthritis. METHODS: Synovial fluid specimens of 78 patients were harvested to detect the levels of interleukin-6 and matrix metaloproteinase-3 using ELISA assay. The severity of osteoarthritis was estimated by the Hospital for Special Surgery (HSS) score. Pearson correlation analysis was adopted to explore the pairwise correlations between interleukin-6, matrix metaloproteinase-3 and HSS score. RESULTS AND CONCLUSION:The levels of interleukin-6 and matrix metaloproteinase-3 in the synovial fluid were (13.1±5.7) and (989.3±429.5) μg/L, respectively; the HSS score was (56.4±12.0) points. Pearson correlation analysis showed that the expression levels of interleukin-6 and matrix metaloproteinase-3 had a significant positive correlation (r=0.70,P< 0.001); the levels of interleukin-6 and matrix metaloproteinase-3 had a negative correlation with the HSS scores (r=-0.70,r=-0.75,P < 0.001). Experimental findings show that there is a positive correlation between interleukin-6 and matrix metaloproteinase-3 in the synovial fluids, and these two cytokines also have a positive correlation with the severity of osteoarthritis, indicating that to measure interleukin-6 and matrix metaloproteinase-3 levels in the synovial fluids of osteoarthritis patients is important for early diagnosis and treatment of osteoarthritis.
