CAJ | 학술논문

目的：探讨膀胱尿路上皮癌组织Krüppel样因子8（ KLF8）以及高迁移率族蛋白A2（ HmGA2）表达水平及其与预后的相关性。方法选取2012年1月—2013年10月在郑州大学第五附属医院就诊的74例膀胱尿路上皮癌患者的肿瘤组织作为研究组，另选取同期在本院行膀胱切开取石术或开放前列腺切除术患者23例的膀胱黏膜作为对照组。采用免疫组化方法检测两组标本组织中KLF8和HmGA2蛋白表达水平。采用Cox回归分析KLF8及HmGA2蛋白的表达水平与患者生存预后的关系。结果研究组KLF8和HmGA2表达水平均高于对照组，差异有统计学意义（ t=3.872，P=0.002；t=4.593，P=0.001）。膀胱尿路上皮癌患者临床分期与KLF8和HmGA2表达水平均呈正相关，差异有统计学意义（rs =0.489、0.561，P<0.05）。患者均随访至2015年4月，67例患者在随访截止时存活，总生存率为90.5%。多元Cox回归分析结果显示，临床病理分期、KLF8和HmGA2表达水平是膀胱尿路上皮癌患者生存预后的影响因素（P<0.05）。结论膀胱尿路上皮癌组织KLF8及HmGA2蛋白的高水平表达是患者生存预后的危险因素。
목적：탐토방광뇨로상피암조직Krüppel양인자8（ KLF8）이급고천이솔족단백A2（ HmGA2）표체수평급기여예후적상관성。방법선취2012년1월—2013년10월재정주대학제오부속의원취진적74례방광뇨로상피암환자적종류조직작위연구조，령선취동기재본원행방광절개취석술혹개방전렬선절제술환자23례적방광점막작위대조조。채용면역조화방법검측량조표본조직중KLF8화HmGA2단백표체수평。채용Cox회귀분석KLF8급HmGA2단백적표체수평여환자생존예후적관계。결과연구조KLF8화HmGA2표체수평균고우대조조，차이유통계학의의（ t=3.872，P=0.002；t=4.593，P=0.001）。방광뇨로상피암환자림상분기여KLF8화HmGA2표체수평균정정상관，차이유통계학의의（rs =0.489、0.561，P<0.05）。환자균수방지2015년4월，67례환자재수방절지시존활，총생존솔위90.5%。다원Cox회귀분석결과현시，림상병리분기、KLF8화HmGA2표체수평시방광뇨로상피암환자생존예후적영향인소（P<0.05）。결론방광뇨로상피암조직KLF8급HmGA2단백적고수평표체시환자생존예후적위험인소。
Objective To investigate the correlation between the expression levels of KLF8 and HmGA2 protein and the prognosis of patients with bladder urothelial carcinoma. Methods We obtained tissues of carcinoma niduses from 74 patients with bladder urothelial carcinoma who received treatment in the Fifth Affiliated Hospital of Zhengzhou University from January 2012 to October 2013 as research group,and we collected the bladder mucous membranes from 23 patients who underwent cystolithotomy or open prostatectomy in the same hospital in the same period as the control group. Immunohistochemical method was employed to examine the expression levels of KLF8 and HmGA2 protein of the two groups. Cox regression analysis was undertaken to investigate the relation between the expression levels of KLF8 and HmGA2 protein and survival prognosis. Results Research group was higher than control group in the expression level of KLF8 and HmGA2(t =3. 872,P =0. 002;t =4. 593,P=0. 001). There was positive correlation between clinical staging of patients with bladder urothelial carcinoma and the expression levels of KLF8 and HmGA2(rs =0. 489,0. 561;P<0. 05). The follow-up visits were finished in April 2015, where the number of surviving patients was 67 with a total survival rate of 90. 5%. The Cox multivariate regression analysis showed that clinical pathological staging and the expression levels of KLF8 and HmGA2 were influencing factors for the survival prognosis of patients with bladder urothelial carcinoma ( P <0. 05 ). Conclusion The high expression levels of KLF8 and HmGA2 protein are risk factors for the survival prognosis of patients with bladder urothelial carcinoma.