CAJ | 학술논문

为了对产气荚膜梭菌 tetB（P）耐药基因进行分析，通过生物软件设计引物，扩增 A 型产气荚膜梭菌 tetB （P）耐药基因，测序后对 tetB （P ）基因核苷酸序列与蛋白结构进行分析．结果表明：分离株 tetB （P ）基因长度为1959 bp，编码652个氨基酸．与参考菌株 CW92、EHE-NE18的核苷酸序列同源性依次为99．7％、99．9％，氨基酸序列同源性依次为99．4％、99．8％．分离株 tetB（P）蛋白亲水性氨基酸较多，无跨膜区，含有4个 N-糖基化位点，1个 cAMP 和 cGMP 依赖性蛋白激酶磷酸化位点，9个蛋白激酶 C 磷酸化位点，12个酪蛋白激酶Ⅱ磷酸化位点，7个N-豆寇酰化位点，1个 ATP／GTP 结合位点基序，1个翻译型鸟苷酸结合域，三级结构主要是由α-螺旋、β折叠和无规则卷曲形成．
위료대산기협막사균 tetB（P）내약기인진행분석，통과생물연건설계인물，확증 A 형산기협막사균 tetB （P）내약기인，측서후대 tetB （P ）기인핵감산서렬여단백결구진행분석．결과표명：분리주 tetB （P ）기인장도위1959 bp，편마652개안기산．여삼고균주 CW92、EHE-NE18적핵감산서렬동원성의차위99．7％、99．9％，안기산서렬동원성의차위99．4％、99．8％．분리주 tetB（P）단백친수성안기산교다，무과막구，함유4개 N-당기화위점，1개 cAMP 화 cGMP 의뢰성단백격매린산화위점，9개단백격매 C 린산화위점，12개락단백격매Ⅱ린산화위점，7개N-두구선화위점，1개 ATP／GTP 결합위점기서，1개번역형조감산결합역，삼급결구주요시유α-라선、β절첩화무규칙권곡형성．
In order to analysis tetB(P )drug resistance gene of Clostridium perfringens ,primers were designed by biology software and were used to amplified tetB(P )gene of Clostridium perfringens type A, the amplified gene was sequenced and the protein structure was analyzed.The results showed that the tetB (P )gene was 1 959 bp long,encoding 652 amino acids.Nucleotide sequence homology of tetB(P )gene with reference strains CW92 and EHE-NE18 were 99.7% and 99.9%,respectively,amino acid sequence homolo-gy were 99.4% and 99.8%.The tetB(P )of isolated strain had more hydrophilic amino acid,no transmem-brane domain,had four N-glycosylation sites,one cAMP and cGMP dependent protein kinase phosphoryla-tion site,nine protein kinase C phosphorylation sites,twelve Casein kinase II phosphorylation sites,seven N-myristoylation sites,one ATP/GTP-binding site motif A,one translational (tr)-type guanine nucleotide-binding (G)domain signature.