中国全科医学
中國全科醫學
중국전과의학
Chinese General Practice
2015年
26期
3173-3176
,共4页
王文艺%丁巍%杨晓红
王文藝%丁巍%楊曉紅
왕문예%정외%양효홍
养鸟者肺%肺泡炎,外源性变应性%白细胞介素8%多态性,单核苷酸
養鳥者肺%肺泡炎,外源性變應性%白細胞介素8%多態性,單覈苷痠
양조자폐%폐포염,외원성변응성%백세포개소8%다태성,단핵감산
Bird fancier′s lung%Alveolitis,extrinsic allergic%Interleukin-8%Polymorphisms,single nucleotide
目的:探讨血清及肺泡灌洗液(BALF)中白介素8(IL-8)蛋白表达水平,及血清中单核苷酸多态性与新疆维吾尔族饲鸽者肺( PBL)遗传易患性的相关性。方法选取2012—2013年在新疆喀什地区有鸽子接触史,且血清学抗体阳性,符合PBL诊断标准的维吾尔族患者32例(病例组);选取有鸽子接触史,但血清学抗体阴性者30例(阴性对照组);选取无鸽子接触史且健康维吾尔族者30例(正常对照组)。采用ELISA分别检测3组受试者血清及BALF中IL-8蛋白表达水平;通过UCSC数据库选取IL-8的15个基因位点,采用Sequenom massARRAY SNP分型技术对所有位点进行基因分型,比较3组IL-8基因型分布。结果3组血清、BALF中IL-8蛋白表达水平比较,差异均有统计学意义(P<0.05);阴性对照组和病例组血清、BALF中IL-8蛋白表达水平均高于正常对照组,病例组血清中IL-8蛋白表达水平高于阴性对照组( P<0.05)。选取的15个基因位点的IL-8基因型分布在3组间不存在基因多态性。结论血清、BALF中IL-8蛋白表达水平的增高可能是肺损伤及肺纤维化的诱因,其基因多态性不增加维吾尔族PBL的发病风险。
目的:探討血清及肺泡灌洗液(BALF)中白介素8(IL-8)蛋白錶達水平,及血清中單覈苷痠多態性與新疆維吾爾族飼鴿者肺( PBL)遺傳易患性的相關性。方法選取2012—2013年在新疆喀什地區有鴿子接觸史,且血清學抗體暘性,符閤PBL診斷標準的維吾爾族患者32例(病例組);選取有鴿子接觸史,但血清學抗體陰性者30例(陰性對照組);選取無鴿子接觸史且健康維吾爾族者30例(正常對照組)。採用ELISA分彆檢測3組受試者血清及BALF中IL-8蛋白錶達水平;通過UCSC數據庫選取IL-8的15箇基因位點,採用Sequenom massARRAY SNP分型技術對所有位點進行基因分型,比較3組IL-8基因型分佈。結果3組血清、BALF中IL-8蛋白錶達水平比較,差異均有統計學意義(P<0.05);陰性對照組和病例組血清、BALF中IL-8蛋白錶達水平均高于正常對照組,病例組血清中IL-8蛋白錶達水平高于陰性對照組( P<0.05)。選取的15箇基因位點的IL-8基因型分佈在3組間不存在基因多態性。結論血清、BALF中IL-8蛋白錶達水平的增高可能是肺損傷及肺纖維化的誘因,其基因多態性不增加維吾爾族PBL的髮病風險。
목적:탐토혈청급폐포관세액(BALF)중백개소8(IL-8)단백표체수평,급혈청중단핵감산다태성여신강유오이족사합자폐( PBL)유전역환성적상관성。방법선취2012—2013년재신강객십지구유합자접촉사,차혈청학항체양성,부합PBL진단표준적유오이족환자32례(병례조);선취유합자접촉사,단혈청학항체음성자30례(음성대조조);선취무합자접촉사차건강유오이족자30례(정상대조조)。채용ELISA분별검측3조수시자혈청급BALF중IL-8단백표체수평;통과UCSC수거고선취IL-8적15개기인위점,채용Sequenom massARRAY SNP분형기술대소유위점진행기인분형,비교3조IL-8기인형분포。결과3조혈청、BALF중IL-8단백표체수평비교,차이균유통계학의의(P<0.05);음성대조조화병례조혈청、BALF중IL-8단백표체수평균고우정상대조조,병례조혈청중IL-8단백표체수평고우음성대조조( P<0.05)。선취적15개기인위점적IL-8기인형분포재3조간불존재기인다태성。결론혈청、BALF중IL-8단백표체수평적증고가능시폐손상급폐섬유화적유인,기기인다태성불증가유오이족PBL적발병풍험。
Objective To investigate the correlation between the protein expression level of IL -8 in serum and BALF,single nucleotide polymorphism in serum and the genetic susceptibility to pigeon breeder′s lung ( PBL ) in Uygurs. Methods From 2012 to 2013,we enrolled 32 subjects Uygurs who had contact with pigeons,had positive serologic antibody and accorded with diagnostic criteria as case group;we enrolled 30 people who had contact with pigeons and were with negative serological antibody as negative control group;we selected 30 healthy uygurs who had no contact with pigeon as normal control group. The subjects were all selected from Kashi Prefecture. ELISA was used to detect the protein expression level of IL-8 in serum and BALF of the subjects. Through UCSC database,we selected 15 genetic loci of IL-8. Sequenom massARRAY SNP typing technology was employed to conduct genetic typing. Comparison was made among the three groups in the genotype distribution of IL-8. Results The three groups were significantly different(P<0. 05)in the protein expression level of IL-8 in serum and BALF;the negative control group and the case group were higher(P<0. 05)than the normal control group in the protein expression level of IL-8 in serum and BALF,and the case group was higher(P<0. 05)than the negative control group in the protein expression level of IL-8 in serum. No gene polymorphism was found in the 15 gene loci of IL-8 of the three groups. Conclusion The increase of protein expression level of IL-8 in serum and BALF may be an incentive to cause lung injury and fibrosis,but gene polymorphism does not increase the risk of PBL in Uygurs.