CAJ | 학술논문

目的：通过对糖类成分的两种色谱方法进行比较，选择一种准确、方便、快速测定注射用丹参（冻干）中糖类成分的方法。方法采用高效液相－蒸发光散射法（HPLC-ELSD），Alltech Prevail-TM Carbohydrate ES （4．6 mm ×250 mm，5μm）色谱柱，乙腈－水（76∶24）流动相等度洗脱，ELSD检测器。采用离子色谱法（IC），CarboPac PA20（3 mm ×150 mm，S ／N 003654）阴离子交换分析色谱柱，CarboPac PA20（3 mm ×50 mm，S ／N 003405）保护柱，以氢氧化钠溶液（7 mmol ／L）洗脱，脉冲安培检测器。以上两种色谱方法分别对注射用丹参（冻干）中糖类成分进行测定。结果在上述色谱条件下，HPLC-ELSD 法可同时测定果糖和葡萄糖2种糖类成分，方法的线性关系、精密度、重复性及回收率等均符合要求；IC 法可同时测定阿拉伯糖、半乳糖、葡萄糖、蔗糖、甘露糖和果糖6种糖类成分，方法经考察准确可靠。两种色谱方法分别测定的果糖和葡萄糖结果基本一致。结论离子色谱法更适合于注射用丹参（冻干）中糖类成分的测定。
목적：통과대당류성분적량충색보방법진행비교，선택일충준학、방편、쾌속측정주사용단삼（동간）중당류성분적방법。방법채용고효액상－증발광산사법（HPLC-ELSD），Alltech Prevail-TM Carbohydrate ES （4．6 mm ×250 mm，5μm）색보주，을정－수（76∶24）류동상등도세탈，ELSD검측기。채용리자색보법（IC），CarboPac PA20（3 mm ×150 mm，S ／N 003654）음리자교환분석색보주，CarboPac PA20（3 mm ×50 mm，S ／N 003405）보호주，이경양화납용액（7 mmol ／L）세탈，맥충안배검측기。이상량충색보방법분별대주사용단삼（동간）중당류성분진행측정。결과재상술색보조건하，HPLC-ELSD 법가동시측정과당화포도당2충당류성분，방법적선성관계、정밀도、중복성급회수솔등균부합요구；IC 법가동시측정아랍백당、반유당、포도당、자당、감로당화과당6충당류성분，방법경고찰준학가고。량충색보방법분별측정적과당화포도당결과기본일치。결론리자색보법경괄합우주사용단삼（동간）중당류성분적측정。
Objective To compare two chromatographic methods and then to select an accurate,conven-ient and rapid method for the determination of sugars in Danshen Injection (lyophilized powder). Methods Firstly,HPLC -ELSD method was applied on an Alltech PrevailTM Carbohydrate ES (4.6 mm ×250 mm,5 μm)column,and acetonitrile-water (76∶24)was used as the mobile phase on an isocrat-ic elution with ELSD as the detector.Apart from that,IC method was adopted on a CarboPac PA20 (150 mm ×3 mm,S /N 003654 )anion exchange column with the CarboPac PA20 (50 ×3 mm,S /N 003405)column as the guard column,and sodium hydrate solution (7 mmol /L)was used as eluent with the pulsed amperometric detector.To sum up,HPLC-ELSD and IC methods described above were ap-plied to determine the sugars in Danshen Injection (lyophilized powder)respectively.Results Under the chromatographic conditions mentioned above,HPLC-ELSD method could simultaneously determine the contents of fructose and glucose.Linear relationship,precision,repeatability and recovery rate of the method were all qualified;IC method could simultaneously determine arabinose,galactose,glucose,su-crose,mannose as well as fructose,and the method proved to be accurate and reliable.The determina-tion results of fructose and glucose by both methods were basically the same.Conclusion IC method seems to be a better choice to determine the monosaccharides and disaccharide in Danshen Injection (ly-ophilized powder).