CAJ | 학술논문

目的：建立测定拟缺香茶菜药材中二萜类成分 Kamebacetal-A 、Kamebacetal-B、肾形香茶菜丙素的方法。方法采用固相萃取-高效液相色谱法（SPE-HPLC）测定3个二萜类成分的含量。使用Venusil C18（4．6 mm ×250 mm，5μm）反相色谱柱；流动相：乙腈（A）－水（B）梯度洗脱；流速：1 mL／min；柱温：30℃；检测波长230 nm。结果拟缺香茶菜中 Kamebacetal-A 、Kamebacetal-B、肾形香茶菜丙素分别在0．056～0．448 g／L（r ＝0．9992），0．0026～0.0208 g／L（r ＝0．9994），0．016～0.128 g／L（r ＝0．9997）内与峰面积成良好的线性关系；平均回收率分别为97．53％（RSD ＝2.16％），96．02％（RSD ＝2．01％），98．23％（RSD ＝2．10％）。结论所建立的方法灵敏、准确、稳定，可用于拟缺香茶菜中 Kamebacetal-A 、Kamebacetal-B、肾形香茶菜丙素的含量测定，为拟缺香茶菜抗肿瘤谱效关系的研究奠定基础。
목적：건립측정의결향다채약재중이첩류성분 Kamebacetal-A 、Kamebacetal-B、신형향다채병소적방법。방법채용고상췌취-고효액상색보법（SPE-HPLC）측정3개이첩류성분적함량。사용Venusil C18（4．6 mm ×250 mm，5μm）반상색보주；류동상：을정（A）－수（B）제도세탈；류속：1 mL／min；주온：30℃；검측파장230 nm。결과의결향다채중 Kamebacetal-A 、Kamebacetal-B、신형향다채병소분별재0．056～0．448 g／L（r ＝0．9992），0．0026～0.0208 g／L（r ＝0．9994），0．016～0.128 g／L（r ＝0．9997）내여봉면적성량호적선성관계；평균회수솔분별위97．53％（RSD ＝2.16％），96．02％（RSD ＝2．01％），98．23％（RSD ＝2．10％）。결론소건립적방법령민、준학、은정，가용우의결향다채중 Kamebacetal-A 、Kamebacetal-B、신형향다채병소적함량측정，위의결향다채항종류보효관계적연구전정기출。
Objective To establish a method to simultaneously determine three diterpenoids in Isodon ex-cisoides.Methods The procedure of SPE-HPLC was performed on a Venusil C18 column(4.6 mm ×250 mm,5 μm).The mobile phases was water and acetonitrile in gradient mode (0 ~12 min,32%A;12 ~18 min,32 ~45%A;18 ~35 min,45%A).The flow rate was 1.0 mL·min -1 .The column tempera-ture was 30℃.The UV detection wave length was 230 nm.Results All the standard compounds showed a good linearity in the range of 0.056 ~0.448 g/L (r =0.999 2),0.002 6 ~0.020 8 g/L (r =0.999 4),0.016 ~0.128 g/L(r =0.999 7)for Kamebacetal-A,Kamebacetal-B,and Reniformin C, respectively.The average recoveries of the three diterpenoids were 97.53%,96.02%,98.23% with RSD 2.16%,2.01%,and 2.10%,respectively.Conclusion This method is simple,accurate and ef-fective.It could be used for quality control of ingredients in Isodon excisoides.It could also provide refer-ence for the study of antitumor activity spectrum.