CAJ | 학술논문

为进一步提高枣产品的附加值，实现枣树的综合开发利用，试验采用紫外分光光度法和高效液相色谱法分别对4种不同采摘时期（盛花后第0天和第7天）的枣叶和枣芽样品进行黄酮含量的测定，并分析了各样品醇提物对 DPPH 自由基的清除效果．结果表明：两种方法均可用于黄酮类化合物的分析检测．不同采摘时间、部位及品种的样品中黄酮含量各不相同，除骏枣叶的黄酮含量略低于骏枣芽外，‘七月’枣、‘大王’枣及‘灰枣’的枣叶黄酮含量均比枣芽中的高．其中6月12日采摘的七月枣叶中黄酮含量最高为18．84 mg／g，其醇提物对 DPPH 自由基的清除率达到43．18％，显著高于对照．说明各样品对 DPPH 自由基均有一定的清除作用，且与其中的黄酮含量高度相关，可做为有效的天然自由基清除剂．
위진일보제고조산품적부가치，실현조수적종합개발이용，시험채용자외분광광도법화고효액상색보법분별대4충불동채적시기（성화후제0천화제7천）적조협화조아양품진행황동함량적측정，병분석료각양품순제물대 DPPH 자유기적청제효과．결과표명：량충방법균가용우황동류화합물적분석검측．불동채적시간、부위급품충적양품중황동함량각불상동，제준조협적황동함량략저우준조아외，‘칠월’조、‘대왕’조급‘회조’적조협황동함량균비조아중적고．기중6월12일채적적칠월조협중황동함량최고위18．84 mg／g，기순제물대 DPPH 자유기적청제솔체도43．18％，현저고우대조．설명각양품대 DPPH 자유기균유일정적청제작용，차여기중적황동함량고도상관，가주위유효적천연자유기청제제．
Four kinds of jujube leave and bud which picked in different period were quantified for fla-vones by ultraviolet spectrophotometry (UV)and High Performance Liquid Chromatography (HPLC), their alcohol extracts to scavenge activity of DPPH were also analyzed.The results showed that both meth-ods (UV and HPLC)could be used for the analysis and detection of flavonoids,HPLC showed higher sen-sitivity and accuracy.Picking periods,parts and varieties made different flavone contents,leaves tended to be higher than buds in cultivars ‘Qiyue’‘Dawang’and ‘Hui’jujube.‘Jun’jujube showed opposite tend-ency,leaves were a little lower than buds.‘Qiyue’leaves picked in July 12 th had the highest amount of flavone components,and its alcohol extracts could scavenge DPPH free radicals for 43.18%,much higher than the control groups,meanwhile the effects of eliminating free radicals submitted positive pertinence with flavone concentration.