国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
17期
2538-2540
,共3页
李静芳%文丽%周友全%郭凤丽%杨丽
李靜芳%文麗%週友全%郭鳳麗%楊麗
리정방%문려%주우전%곽봉려%양려
尿沉渣分析仪%尿沉渣细菌定量%尿培养%受试者工作特征曲线
尿沉渣分析儀%尿沉渣細菌定量%尿培養%受試者工作特徵麯線
뇨침사분석의%뇨침사세균정량%뇨배양%수시자공작특정곡선
U F-1000i urinary sediment analyzer%urinary tract infection%urine culture%receiver operating characteristic curve
目的:评价尿沉渣细菌定量检查作为筛选尿路感染指标的可行性。方法收集门诊及住院患者标本191例,先做尿培养,用余下的尿液做尿沉渣细菌定量检查,以细菌培养结果为标准,绘制受试者工作特征曲线(ROC)曲线,求出白细胞和细菌计数对尿路感染的诊断阈值,并计算其灵敏度、特异度、准确率、假阴性/假阳性率和阴/阳性预测值。结果尿细菌培养阳性的标本占39.7%,其中最常见的致病菌是大肠埃希菌;尿路感染的诊断阈值,细菌计数为1024.5个/微升,白细胞计数为135.8个/微升。白细胞和细菌计数联合测定对尿路感染检查的最佳灵敏度为62.5%、特异度为98.1%、阳性预测值为95.7%、阴性预测值为79.6%、假阳性率为1.9%、假阴性率为37.5%、准确率为83.8%。结论尿沉渣细菌定量计数联合白细胞计数可作为泌尿系统感染的一项快速筛查指标,可筛除大部分阴性结果,尤其对细菌培养阳性结果的预测性较高,但仍然不可替代定量细菌培养。
目的:評價尿沉渣細菌定量檢查作為篩選尿路感染指標的可行性。方法收集門診及住院患者標本191例,先做尿培養,用餘下的尿液做尿沉渣細菌定量檢查,以細菌培養結果為標準,繪製受試者工作特徵麯線(ROC)麯線,求齣白細胞和細菌計數對尿路感染的診斷閾值,併計算其靈敏度、特異度、準確率、假陰性/假暘性率和陰/暘性預測值。結果尿細菌培養暘性的標本佔39.7%,其中最常見的緻病菌是大腸埃希菌;尿路感染的診斷閾值,細菌計數為1024.5箇/微升,白細胞計數為135.8箇/微升。白細胞和細菌計數聯閤測定對尿路感染檢查的最佳靈敏度為62.5%、特異度為98.1%、暘性預測值為95.7%、陰性預測值為79.6%、假暘性率為1.9%、假陰性率為37.5%、準確率為83.8%。結論尿沉渣細菌定量計數聯閤白細胞計數可作為泌尿繫統感染的一項快速篩查指標,可篩除大部分陰性結果,尤其對細菌培養暘性結果的預測性較高,但仍然不可替代定量細菌培養。
목적:평개뇨침사세균정량검사작위사선뇨로감염지표적가행성。방법수집문진급주원환자표본191례,선주뇨배양,용여하적뇨액주뇨침사세균정량검사,이세균배양결과위표준,회제수시자공작특정곡선(ROC)곡선,구출백세포화세균계수대뇨로감염적진단역치,병계산기령민도、특이도、준학솔、가음성/가양성솔화음/양성예측치。결과뇨세균배양양성적표본점39.7%,기중최상견적치병균시대장애희균;뇨로감염적진단역치,세균계수위1024.5개/미승,백세포계수위135.8개/미승。백세포화세균계수연합측정대뇨로감염검사적최가령민도위62.5%、특이도위98.1%、양성예측치위95.7%、음성예측치위79.6%、가양성솔위1.9%、가음성솔위37.5%、준학솔위83.8%。결론뇨침사세균정량계수연합백세포계수가작위비뇨계통감염적일항쾌속사사지표,가사제대부분음성결과,우기대세균배양양성결과적예측성교고,단잉연불가체대정량세균배양。
Objective To evaluate quantitative examination of urinary sediment bacteria as a basic feasibility of screening indicators for urinary tract infection .Methods 191 outpatients and inpatient specimens were gathered firstly to implement a urine culture ,and then the rest of the urine were used for sediment bacteria quantitative testing .Meanwhile ,bacterial culture was conducted as the standard .According to the results of bacterial culture ,receiver operating characteristic(ROC) was drawn ,the threshold values of leukocyte and bacteria counts for diagnosis of urinary tract infection were found out and its sensitivity ,specificity ,positive / negative predictive value ,false positive/false negative rate and accuracy were calculated .Results The positive rate of urine culture was 39 .7% ,and the most common pathogen was Escherichia coli .The threshold value of bacteria and leukocyte counts for diagnosis of urinary tract infection was 1 024 .5/μL and 135 .8/μL respectively .When combined leukocyte and bacteria counts for urinary tract infection ,the optimum sensitivity was 62 .5% ,specificity was 98 .1% ,positive predictive value was 95 .7% ,negative predictive value was 79 .6% ,false positive rate was 1 .9% ,false negative rate was 37 .5% ,and accuracy was 83 .8% .Conclusion With UF‐1000i urinary sediment analyzer ,the combined determination of leukocyte and bacteria counts can remove the great mass of negative results ,Especially the results of bacterial culture positive predictability is higher ,but still can not replace of quantitative bacterial culture .