CAJ | 학술논문

目的：探讨滋补脾阴方药调节糖尿病脾阴虚大鼠下丘脑内质网应激的作用机制。方法将大鼠随机分为空白组，糖尿病组，脾阴虚组，糖尿病脾阴虚组，糖尿病脾阴虚＋滋补脾阴方药组共5组。通过蛋白免疫印迹（Western blot）法观察各组大鼠下丘脑内质网应激标志分子 RNA 依赖的蛋白激酶样内质网激酶（PERK）、真核起始因子2的α亚单位（eIF2α）、葡萄糖调节蛋白78（GRP78）的变化。通过逆转录聚合酶链反应（RT-PCR）法观察 GRP78 mRNA 表达变化，评价滋补脾阴方药对内质网应激的影响。结果糖尿病组、糖尿病脾阴虚组大鼠下丘脑 p-PERK、p-eIF2α、GRP78蛋白表达较空白组均增加（P ＜0．05），糖尿病脾阴虚＋滋补脾阴方药组较糖尿病组、糖尿病脾阴虚组减弱（P ＜0．05），eIF2α蛋白水平各组间无统计学差异。糖尿病组、脾阴虚组、糖尿病脾阴虚组大鼠下丘脑 GRP78 mRNA 表达较空白组均增强（P ＜0．05），糖尿病脾阴虚＋滋补脾阴方药组下丘脑GRP78 mRNA 与糖尿病组、糖尿病脾阴虚组比较均有所降低（P ＜0.05）。结论糖尿病组、糖尿病脾阴虚组大鼠下丘脑中发生内质网应激，滋补脾阴方药具有减轻内质网应激的作用。
목적：탐토자보비음방약조절당뇨병비음허대서하구뇌내질망응격적작용궤제。방법장대서수궤분위공백조，당뇨병조，비음허조，당뇨병비음허조，당뇨병비음허＋자보비음방약조공5조。통과단백면역인적（Western blot）법관찰각조대서하구뇌내질망응격표지분자 RNA 의뢰적단백격매양내질망격매（PERK）、진핵기시인자2적α아단위（eIF2α）、포도당조절단백78（GRP78）적변화。통과역전록취합매련반응（RT-PCR）법관찰 GRP78 mRNA 표체변화，평개자보비음방약대내질망응격적영향。결과당뇨병조、당뇨병비음허조대서하구뇌 p-PERK、p-eIF2α、GRP78단백표체교공백조균증가（P ＜0．05），당뇨병비음허＋자보비음방약조교당뇨병조、당뇨병비음허조감약（P ＜0．05），eIF2α단백수평각조간무통계학차이。당뇨병조、비음허조、당뇨병비음허조대서하구뇌 GRP78 mRNA 표체교공백조균증강（P ＜0．05），당뇨병비음허＋자보비음방약조하구뇌GRP78 mRNA 여당뇨병조、당뇨병비음허조비교균유소강저（P ＜0.05）。결론당뇨병조、당뇨병비음허조대서하구뇌중발생내질망응격，자보비음방약구유감경내질망응격적작용。
Objective To investigate the effects of spleen-yin nourishing formula,ZiBu PiYin Recipe (ZBPYR),on endoplasmic reticulum stress (ERS)in the hypothalamus of spleen yin deficiency diabetic rats.Methods Rats were randomly divided into five groups:control (cont)group,Diabetes Mellitus (DM)group,spleen yin deficiency (pi)group,piDMgroup,and ZBPYR group.The expressions of p-PERK,p-eIF2α,eIF2αand GRP78,which were endoplasmic reticulum stress markers in the hypothala-mus of rats,were measured using western blotting methods.Results The protein expressions of p-PERK,p-eIF2αand GRP78 in the hypothalamus of DM group and piDM group were elevated,as com-pared with cont group (P <0.05).Their levles were decreased in the ZBPYR group,compared with DM group and piDMgroup (P <0.05).In addition,no significant difference in eIF2αexpression was detec-ted among different groups.Compared with the cont group,GRP78 mRNA expression in hypothalamus increased in the DMgroup,pi group and piDMgroup (P <0.05).The expression of GRP78 mRNA was lower in ZBPYR group than in the DMand piDMgroups (P <0.05).Conclusion Endoplasmic reticu-lum stress seems to be present in the hypothalamus in the DM and piDM groups.Formulas that enrich and tonify the spleen yin have the effects of alleviating endoplasmic reticulum stress.