中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
Chinese Journal of Neuromedicine
2015年
8期
770-774
,共5页
罗振钊%孔曼%卢忠心%王静
囉振釗%孔曼%盧忠心%王靜
라진쇠%공만%로충심%왕정
缺血性脑卒中%miR-145%miR-497%超敏C反应蛋白%基质金属蛋白酶-9
缺血性腦卒中%miR-145%miR-497%超敏C反應蛋白%基質金屬蛋白酶-9
결혈성뇌졸중%miR-145%miR-497%초민C반응단백%기질금속단백매-9
Ischemic stroke%MiR-145%MiR-497%High sensitive C-reactive protein%Matrix metalloproteinase-9
目的 探讨miR-145、miR-497在缺血性脑卒中患者血清中的表达水平及其表达变化和超敏C反应蛋白(Hs-CRP)、基质金属蛋白酶-9(MMP-9)的相关性. 方法 选择武汉市中心医院自2013年1月至10月收治的126例缺血性脑卒中患者(患者组,包括轻度神经功能缺损28例、中度神经功能缺损61例、重度神经功能缺损37例)和同期107例健康体检者(对照组)为研究对象,采用荧光定量PCR法检测血清中miR-145、miR-497的表达水平,采用免疫比浊法及ELISA法分别检测Hs-CRP和MMP-9的表达水平,采用偏相关分析法探索患者组血清中miR-145、miR-497的表达变化与Hs-CRP、MMP-9的相关性. 结果 (1)患者组血清中miR-145、miR-497及Hs-CRP、MMP-9的表达水平明显高于对照组,差异均有统计学意义(P＜0.05).偏相关分析法结果显示:患者组血清中miR-145的表达水平与miR-497、CRP、MMP-9都存在正相关性(r=0.718,P=0.000;r=0.658,P=0.000;r=0.455,p=0.000),miR-497的表达水平与CRP、MMP-9都存在正相关性(r=0.845,p=0.000;r=0.787,P=0.000).(2)重度神经功能缺损患者血清中miR-145、miR-497及Hs-CRP、MMP-9的表达水平明显高于中度、轻度患者,中度神经功能缺损患者血清中miR-145、miR-497及Hs-CRP、MMP-9的表达水平明显高于轻度患者,差异均有统计学意义(P＜0.05);并且在不同程度神经功能缺损患者血清中,miR-145、miR-497和Hs-CRP、MMP-9的表达水平之间具有不同的相关强度. 结论 患者血清中miR-145、miR-497可能可以作为诊断缺血性脑卒中的分子标志物,并且二者在缺血性脑卒中的发生发展过程中可能具有协同调控作用.
目的 探討miR-145、miR-497在缺血性腦卒中患者血清中的錶達水平及其錶達變化和超敏C反應蛋白(Hs-CRP)、基質金屬蛋白酶-9(MMP-9)的相關性. 方法 選擇武漢市中心醫院自2013年1月至10月收治的126例缺血性腦卒中患者(患者組,包括輕度神經功能缺損28例、中度神經功能缺損61例、重度神經功能缺損37例)和同期107例健康體檢者(對照組)為研究對象,採用熒光定量PCR法檢測血清中miR-145、miR-497的錶達水平,採用免疫比濁法及ELISA法分彆檢測Hs-CRP和MMP-9的錶達水平,採用偏相關分析法探索患者組血清中miR-145、miR-497的錶達變化與Hs-CRP、MMP-9的相關性. 結果 (1)患者組血清中miR-145、miR-497及Hs-CRP、MMP-9的錶達水平明顯高于對照組,差異均有統計學意義(P＜0.05).偏相關分析法結果顯示:患者組血清中miR-145的錶達水平與miR-497、CRP、MMP-9都存在正相關性(r=0.718,P=0.000;r=0.658,P=0.000;r=0.455,p=0.000),miR-497的錶達水平與CRP、MMP-9都存在正相關性(r=0.845,p=0.000;r=0.787,P=0.000).(2)重度神經功能缺損患者血清中miR-145、miR-497及Hs-CRP、MMP-9的錶達水平明顯高于中度、輕度患者,中度神經功能缺損患者血清中miR-145、miR-497及Hs-CRP、MMP-9的錶達水平明顯高于輕度患者,差異均有統計學意義(P＜0.05);併且在不同程度神經功能缺損患者血清中,miR-145、miR-497和Hs-CRP、MMP-9的錶達水平之間具有不同的相關彊度. 結論 患者血清中miR-145、miR-497可能可以作為診斷缺血性腦卒中的分子標誌物,併且二者在缺血性腦卒中的髮生髮展過程中可能具有協同調控作用.
목적 탐토miR-145、miR-497재결혈성뇌졸중환자혈청중적표체수평급기표체변화화초민C반응단백(Hs-CRP)、기질금속단백매-9(MMP-9)적상관성. 방법 선택무한시중심의원자2013년1월지10월수치적126례결혈성뇌졸중환자(환자조,포괄경도신경공능결손28례、중도신경공능결손61례、중도신경공능결손37례)화동기107례건강체검자(대조조)위연구대상,채용형광정량PCR법검측혈청중miR-145、miR-497적표체수평,채용면역비탁법급ELISA법분별검측Hs-CRP화MMP-9적표체수평,채용편상관분석법탐색환자조혈청중miR-145、miR-497적표체변화여Hs-CRP、MMP-9적상관성. 결과 (1)환자조혈청중miR-145、miR-497급Hs-CRP、MMP-9적표체수평명현고우대조조,차이균유통계학의의(P＜0.05).편상관분석법결과현시:환자조혈청중miR-145적표체수평여miR-497、CRP、MMP-9도존재정상관성(r=0.718,P=0.000;r=0.658,P=0.000;r=0.455,p=0.000),miR-497적표체수평여CRP、MMP-9도존재정상관성(r=0.845,p=0.000;r=0.787,P=0.000).(2)중도신경공능결손환자혈청중miR-145、miR-497급Hs-CRP、MMP-9적표체수평명현고우중도、경도환자,중도신경공능결손환자혈청중miR-145、miR-497급Hs-CRP、MMP-9적표체수평명현고우경도환자,차이균유통계학의의(P＜0.05);병차재불동정도신경공능결손환자혈청중,miR-145、miR-497화Hs-CRP、MMP-9적표체수평지간구유불동적상관강도. 결론 환자혈청중miR-145、miR-497가능가이작위진단결혈성뇌졸중적분자표지물,병차이자재결혈성뇌졸중적발생발전과정중가능구유협동조공작용.
Objective To explore the changes of serum miR-145 and miR-497 levels and their correlations with high sensitive C-reactive protein (Hs-CRP) and matrix metalloproteinase-9 (MMP-9) in patients with ischemic stroke.Methods One hundred and twenty-six patients with ischemic stroke,including 28 mild neurologic impairment (NI) patients,61 moderate NI patients and 37 severe NI patients,and 107 normal controls,were collected in our hospital from January 2013 to October 2013.Expressions of serum miR-145 and miR-497 were measured by real-time fluorescent quantitative PCR,protein markers Hs-CRP and MMP-9 were determined by immune turbidimetry and ELISA assay,respectively,correlations between microRNAs (miRNAs) and protein markers were analyzied by partial correlation analysis.Results Both expressions of serum miR-145 and miR-497,and Hs-CRP and MMP-9 levels in ischemic stroke patients were significantly elevated as compared with those in the normal controls (P＜0.05).Positive correlations were noted between of serum miR-145 expression and both serum miR-497 expression and expressions of serum Hs-CRP and MMP-9 (r=0.718,P=0.000;r=0.658,P=0.000;r=0.455,P=0.000);meanwhile,serum miR-497 expression and expressions of serum Hs-CRP and MMP-9 were also positively correlated (r=0.845,P=0.000;r=0.787,P=0.000).Severe NI patients had significantly higher expressions of serum miR-145 and miR-497,and Hs-CRP and MMP-9 levels as compared with mild NI patients and moderate NI patients (P＜0.05),and these levels in the moderate NI patients were significantly higher than those in the mild NI patients (P＜ 0.05);these miRNAs and protein markers showed a different correlation strength depended on the different severities of neurologic impairment.Conclusion Both of serum miR-145 and miR-497 levels may be useful biomarkers for screening ofischemic stroke;and miR-145 and miR-497 may have synergistic effect on regulating the pathogenesis of ischemic stroke.
