CAJ | 학술논문

为研究磁场对脑片海马区自发兴奋性突触后电流（sEPSC）的生物刺激作用，利用实际测量、数学建模和Comsol软件对典型磁刺激装置进行建模与仿真，确定磁场暴露区的磁场强度和分布特性，并采用膜片钳实验对磁场刺激条件下大鼠离体脑片海马区sEPSC进行了研究。结果表明：磁场强度从刺激器舌面处到脑片区衰减125倍左右，且磁场进入溶液后分布更加均匀；磁场强度越高，sEPSC的幅值和频率越低，当磁场强度达到2 mT时，神经元sEPSC的幅值、频率、半波长、上升时间和衰减时间显著下降，说明随磁场磁剂量增加，磁场抑制sEPSC的发放。
위연구자장대뇌편해마구자발흥강성돌촉후전류（sEPSC）적생물자격작용，이용실제측량、수학건모화Comsol연건대전형자자격장치진행건모여방진，학정자장폭로구적자장강도화분포특성，병채용막편겸실험대자장자격조건하대서리체뇌편해마구sEPSC진행료연구。결과표명：자장강도종자격기설면처도뇌편구쇠감125배좌우，차자장진입용액후분포경가균균；자장강도월고，sEPSC적폭치화빈솔월저，당자장강도체도2 mT시，신경원sEPSC적폭치、빈솔、반파장、상승시간화쇠감시간현저하강，설명수자장자제량증가，자장억제sEPSC적발방。
In order to investigate the biological stimulation of the magnetic field on the hippocampal spontaneous Excitatory Postsynaptic Current (sEPSC) of the brain slices, the actual measurement, mathematical modeling and Comsol software simulation were used to model and simulate a typical magnetic stimulation device, the magnetic field in-tensity and distribution characteristics of the exposed area were confirmed. And the hippocampal sEPSC in the vitro brain slices under the magnetic field was studied with the patch clamp experiment. The results showed that the magnetic field intensity decreases by 125 times from the simulator to the brain slice, and the magnetic field distributes uniformly in physiological solution. The higher the magnetic field dose, the lower the amplitude and frequency of sEPSC. When the magnetic field intensity reaches 2 mT, the amplitude, fequency, half-wave width, the rise time and decay time of neuron sEPSC decrease significantly. This results illustrated that with the increase of intensity of magnetic field, the magnetic field suppresses the sEPSC release.