CAJ | 학술논문

目的：观察不同生物状态白色念珠菌对口腔上皮细胞的黏附能力及ALS mRNA表达,以期揭示口腔白色念球菌感染机制。方法将白色念珠菌3683、SC5314、3630与来源于50名健康志愿者的口腔上皮细胞混合培养,采用革兰阳性染色观察白色念珠菌的黏附能力,采用荧光定量RT-PCR法检测白色念珠菌3683、SC5314、3630中ALS2及ALS3 mRNA表达情况。采用SPSS 15.0统计学软件进行数据分析。结果黏附实验结果显示,3株白色念珠菌均可黏附于口腔上皮细胞,且菌株3683黏附数量明显多于菌株SC5314和菌株3630,统计学比较显示,差异有统计学意义(P<0.05),而菌株SC5314和菌株3630黏附数量比较,差异无统计学意义(P>0.05)。荧光定量RT-PCR结果显示,白色念珠菌3683、SC5314、3630中均能检测到ALS2及ALS3 mRNA表达,其中,菌株3683 ALS2及ALS3 mRNA表达水平均高于菌株SC5314和菌株3630,统计学比较显示,差异有统计学意义(P<0.05)；菌株3630 ALS2及ALS3 mRNA表达水平均高于菌株SC5314,但两者比较,差异无统计学意义(P>0.05)。结论不同生物状态白色念珠菌的口腔上皮细胞黏附能力不同,菌株黏附能力的强弱可能与其ALS2及ALS3基因情况表达相关。
목적：관찰불동생물상태백색념주균대구강상피세포적점부능력급ALS mRNA표체,이기게시구강백색념구균감염궤제。방법장백색념주균3683、SC5314、3630여래원우50명건강지원자적구강상피세포혼합배양,채용혁란양성염색관찰백색념주균적점부능력,채용형광정량RT-PCR법검측백색념주균3683、SC5314、3630중ALS2급ALS3 mRNA표체정황。채용SPSS 15.0통계학연건진행수거분석。결과점부실험결과현시,3주백색념주균균가점부우구강상피세포,차균주3683점부수량명현다우균주SC5314화균주3630,통계학비교현시,차이유통계학의의(P<0.05),이균주SC5314화균주3630점부수량비교,차이무통계학의의(P>0.05)。형광정량RT-PCR결과현시,백색념주균3683、SC5314、3630중균능검측도ALS2급ALS3 mRNA표체,기중,균주3683 ALS2급ALS3 mRNA표체수평균고우균주SC5314화균주3630,통계학비교현시,차이유통계학의의(P<0.05)；균주3630 ALS2급ALS3 mRNA표체수평균고우균주SC5314,단량자비교,차이무통계학의의(P>0.05)。결론불동생물상태백색념주균적구강상피세포점부능력불동,균주점부능력적강약가능여기ALS2급ALS3기인정황표체상관。
Objective To observe the adhesion ability of Candida albicans with different biological states for oral ep-ithelial cells and its ALS mRNA expression, in order to reveal the mechanism of oral Candida albicans infection. Methods Candida albicans 3683, SC5314, 3630 and oral epithelial cells from 50 cases of healthy volunteers were mixed cultivation. Gram positive staining was used to observe the adhesion ability of Candida albicans. Candida albi-cans 3683, SC5314, 3630 ALS2 and ALS3 mRNA expressions were detected by fluorescent quantitation RT-PCR method. SPSS 15.0 statistical software was used for data analysis. Results Adhesion experiment results showed that Candida albicans 3683, SC5314, 3630 could stick to oral epithelial cells. Adhesion level of Candida albicans 3683 was higher than that of Candida albicans SC5314 and 3630, the differences were statistically significant (P<0.05). The ad-hesion level between Candida albicans SC5314 and 3630 had no statistically significant difference (P>0.05). Fluores-cent quantitation RT-PCR results showed that Candida albicans 3683, SC5314, 3630 could express ALS2 and ALS3 mRNA. The ALS2 and ALS3 mRNA expressions in Candida albicans 3683 were higher than those of Candida albicans SC5314 and 3630, the differences were statistically significant (P<0.05). The ALS2 and ALS3 mRNA expressions be-tween Candida albicans SC5314 and 3630 had no statistically significant difference (P > 0.05). Conclusion Candida albicans with different biological states have different adhesion abilities for oral epithelial cells, which can be related with the expressions of ALS2 and ALS3 mRNA.