CAJ | 학술논문

目的评估轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条的灵敏度和特异性.方法以培养的轮状病毒、肠道腺病毒、以及其他肠道病原体,还有140例具有胃肠道感染症状患者的粪便标本,分别用轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条和轮状病毒-腺病毒抗原二合一彩色微球法检测试纸条检测.结果轮状病毒-肠道腺病毒抗原荧光纳米颗粒快速联检试纸条的灵敏度显著高于轮状病毒-腺病毒抗原二合一彩色微球法检测试纸条;对常见的柯萨奇病毒(A16)、埃可病毒(30型)和肠道病毒(EV71)无交叉反应.结论轮状病毒-肠道腺病毒抗原的荧光纳米颗粒快速联检试纸条灵敏度显著高于彩色微球法快速检测试纸条,特异性良好.
목적 평고륜상병독-장도선병독항원형광납미과립쾌속련검시지조적령민도화특이성.방법 이배양적륜상병독、장도선병독、이급기타장도병원체,환유140례구유위장도감염증상환자적분편표본,분별용륜상병독-장도선병독항원형광납미과립쾌속련검시지조화륜상병독-선병독항원이합일채색미구법검측시지조검측.결과 륜상병독-장도선병독항원형광납미과립쾌속련검시지조적령민도현저고우륜상병독-선병독항원이합일채색미구법검측시지조;대상견적가살기병독(A16)、애가병독(30형)화장도병독(EV71)무교차반응.결론 륜상병독-장도선병독항원적형광납미과립쾌속련검시지조령민도현저고우채색미구법쾌속검측시지조,특이성량호.
Objective To evaluate the sensitivity and specificity of rotavirus and enteric adenovirus antigen fluorescent nanoparticles rapid combined detection test strip.Methods The specimen including rotavirus,enteric adenovirus,and other enteric pathogens and 140 cases of patients with symptoms of gastrointestinal infection.The performance was analyzed after fluorescent nanoparticles rapid combined detection test strip and coloredmicrospheres rapid detection test strips were used to detect the same sample.Result The sensitivity of fluorescent nanoparticles rapid combined detection test strip is significantly higher than coloredmicrospheres rapid test strip and no specific reaction with Coxsachievirus (type A16),ECHO virus (type 30) and enterovirus (type 71).Conclusion The sensitivity of fluorescent nanoparticles rapid combined detection test strips is significantly higher than coloredmicrospheres rapid detection test strip,and the specificity is good.