CAJ | 학술논문

目的了解2008-2012年湖北省流行麻疹野病毒的基因特征.方法采集2008-2012年间麻疹暴发或散发病例的咽拭子标本,使用Vero/SLAM细胞进行病毒分离,并对分离到的麻疹病毒进行基因型的鉴定和基因亲缘关系分析.结果 2008-2012年从湖北省共9个地市采集的339份临床标本中分离到26株麻疹病毒株,均属于麻疹野病毒H1基因型中的H1a基因亚型,未发现输入基因型麻疹病毒.26株H1a基因亚型麻疹病毒株的核苷酸和氨基酸之间的同源性分别为97.1％～100％和95.3％～100％.与我国疫苗株(Shanghai191株)相比,其核苷酸和氨基酸同源性分别为91.8％～92.5％和87.4％～90.0％.基因亲缘性分析结果显示,26株麻疹病毒属于两个不同的分支,为两个不同的麻疹病毒传播链,并与其他省份流行的麻疹病毒在基因亲缘关系和流行时间上都很接近.结论 H1a基因亚型麻疹病毒在湖北省持续传播,湖北省麻疹病毒与我国其他各省流行的麻疹病毒在共同进化.本研究为湖北省控制和消除麻疹工作提供了重要的科学依据.
목적 료해2008-2012년호북성류행마진야병독적기인특정.방법 채집2008-2012년간마진폭발혹산발병례적인식자표본,사용Vero/SLAM세포진행병독분리,병대분리도적마진병독진행기인형적감정화기인친연관계분석.결과 2008-2012년종호북성공9개지시채집적339빈림상표본중분리도26주마진병독주,균속우마진야병독H1기인형중적H1a기인아형,미발현수입기인형마진병독.26주H1a기인아형마진병독주적핵감산화안기산지간적동원성분별위97.1％～100％화95.3％～100％.여아국역묘주(Shanghai191주)상비,기핵감산화안기산동원성분별위91.8％～92.5％화87.4％～90.0％.기인친연성분석결과현시,26주마진병독속우량개불동적분지,위량개불동적마진병독전파련,병여기타성빈류행적마진병독재기인친연관계화류행시간상도흔접근.결론 H1a기인아형마진병독재호북성지속전파,호북성마진병독여아국기타각성류행적마진병독재공동진화.본연구위호북성공제화소제마진공작제공료중요적과학의거.
Objective To understand the genetic characteristics of wild measles virus in Hubei province during 2008-2012.Methods To collect throat swab specimens from measles outbreak or sporadic cases in Hubei province during 2008-2012,and perform viral isolation using Vero/SLAM cell,then genotype identification and phylogenetic analysis were performed.Results 26 measles virus strains were isolated from clinical specimens collected in the 9 cities in Hubei province from 2008-2012,they all belong to H1a subgenotype within the wild type measles viruses of H1 genotype,no imported genotypes were found.The nucleotide and amino acid identities among 26 measles virus strains of H1 a subgenotype were for 97.1％-100％ and 95.3％-100％,respectively.And compared with the Chinese vaccine strain (Shanghai191 strain),the nucleotide and amino acid identity were 91.8％-92.5％ and 87.4％-90.0％,respectively.Genetic phylogenetic analysis showed that all 26 measles virus strains belong to two different evolutionary branches,represent two transmission chains,and close to the measles virus isolated in other provinces in genetic phylogeny and endemic time.Conclusion H la subgenotype of measles virus continues to spread in Hubei province from 2008-2012,the measles viruses in Hubei province co-evolved with the viruses in other provinces in China.This study provides an important scientific basis for measles elimination in Hubei province.